Bhendi yellow vein mosaic virus [2005:Thadagan:India]
Average proteome isoelectric point is 7.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3TU33|C3TU33_9GEMI AC4 OS=Bhendi yellow vein mosaic virus [2005:Thadagan:India] OX=611724 GN=AC4 PE=3 SV=1
MM1 pKa = 7.23 PPKK4 pKa = 10.39 RR5 pKa = 11.84 FVINSKK11 pKa = 10.8 NYY13 pKa = 8.9 FLTYY17 pKa = 8.65 PQCSLNKK24 pKa = 10.23 EE25 pKa = 4.04 EE26 pKa = 6.15 ALSQLQNIQTPTNKK40 pKa = 10.0 KK41 pKa = 8.87 YY42 pKa = 10.85 IKK44 pKa = 9.66 ICRR47 pKa = 11.84 EE48 pKa = 3.53 LHH50 pKa = 6.26 EE51 pKa = 4.93 NGEE54 pKa = 4.28 PHH56 pKa = 6.66 LHH58 pKa = 6.03 VLIQFEE64 pKa = 4.78 GKK66 pKa = 9.95 YY67 pKa = 9.77 KK68 pKa = 10.46 CQNQRR73 pKa = 11.84 FFDD76 pKa = 4.08 LVATNRR82 pKa = 11.84 SAHH85 pKa = 5.42 FHH87 pKa = 6.51 PNIQGAEE94 pKa = 3.99 SSSDD98 pKa = 3.15 VKK100 pKa = 11.29 SYY102 pKa = 10.79 IDD104 pKa = 3.7 KK105 pKa = 11.33 DD106 pKa = 3.49 GDD108 pKa = 3.67 TLEE111 pKa = 4.22 WGGFQIDD118 pKa = 3.41 GRR120 pKa = 11.84 SARR123 pKa = 11.84 GGQQSANDD131 pKa = 3.81 AYY133 pKa = 10.7 AAALNTGSKK142 pKa = 10.8 AEE144 pKa = 3.9 ALRR147 pKa = 11.84 VIKK150 pKa = 10.26 EE151 pKa = 3.65 IAPTDD156 pKa = 3.95 FQPQFHH162 pKa = 7.33 DD163 pKa = 4.39 LDD165 pKa = 5.13 PNLHH169 pKa = 7.0 RR170 pKa = 11.84 YY171 pKa = 7.4 FQEE174 pKa = 4.22 PPAPYY179 pKa = 8.43 VTPFSSSSFDD189 pKa = 3.41 QVPEE193 pKa = 3.95 EE194 pKa = 4.16 LEE196 pKa = 3.67 EE197 pKa = 4.08 WACKK201 pKa = 10.63 NFVNAAARR209 pKa = 11.84 PLRR212 pKa = 11.84 PQSIAIEE219 pKa = 4.11 VDD221 pKa = 2.9 TRR223 pKa = 11.84 TGKK226 pKa = 8.61 TMWARR231 pKa = 11.84 SLGPHH236 pKa = 6.71 NYY238 pKa = 10.18 LCGHH242 pKa = 7.38 LDD244 pKa = 4.18 LSPKK248 pKa = 10.15 VYY250 pKa = 11.09 SNDD253 pKa = 2.39 AWFNIIDD260 pKa = 4.53 DD261 pKa = 4.12 VDD263 pKa = 3.5 PHH265 pKa = 5.85 YY266 pKa = 11.0 LKK268 pKa = 10.7 HH269 pKa = 6.2 FKK271 pKa = 10.7 EE272 pKa = 4.46 FMGAQRR278 pKa = 11.84 DD279 pKa = 3.81 WQSNTKK285 pKa = 9.6 YY286 pKa = 10.57 GKK288 pKa = 9.12 PVQIKK293 pKa = 10.41 GGIPTIFLCNPGPNASYY310 pKa = 11.17 KK311 pKa = 10.64 EE312 pKa = 3.98 FLDD315 pKa = 3.76 EE316 pKa = 4.66 EE317 pKa = 4.6 KK318 pKa = 11.17 NSALKK323 pKa = 10.45 AWALKK328 pKa = 9.77 NAAFVFLTEE337 pKa = 4.27 PLYY340 pKa = 11.31 SGTNQSTAQGSEE352 pKa = 3.87 EE353 pKa = 3.99 THH355 pKa = 5.98 QEE357 pKa = 3.91 EE358 pKa = 4.9 EE359 pKa = 4.36 SRR361 pKa = 11.84 SS362 pKa = 3.67
Molecular weight: 41.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.695
IPC2_protein 5.664
IPC_protein 5.703
Toseland 6.109
ProMoST 6.033
Dawson 5.957
Bjellqvist 5.97
Wikipedia 5.957
Rodwell 5.944
Grimsley 6.275
Solomon 5.957
Lehninger 5.944
Nozaki 6.224
DTASelect 6.402
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.326
Patrickios 4.05
IPC_peptide 5.97
IPC2_peptide 6.313
IPC2.peptide.svr19 6.27
Protein with the highest isoelectric point:
>tr|C3TU29|C3TU29_9GEMI AC5 OS=Bhendi yellow vein mosaic virus [2005:Thadagan:India] OX=611724 GN=AC5 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 10.21 RR4 pKa = 11.84 AADD7 pKa = 3.55 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFGSPYY27 pKa = 8.67 TSRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 9.91 QQAWTNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.8 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.53 VTRR97 pKa = 11.84 GVGLTHH103 pKa = 7.8 RR104 pKa = 11.84 IGKK107 pKa = 8.54 RR108 pKa = 11.84 FCVKK112 pKa = 9.96 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.83 IWMDD123 pKa = 3.42 EE124 pKa = 3.91 NNKK127 pKa = 8.38 TKK129 pKa = 10.84 NHH131 pKa = 5.96 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.83 RR143 pKa = 11.84 RR144 pKa = 11.84 PTDD147 pKa = 3.06 KK148 pKa = 10.78 PQDD151 pKa = 3.59 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.61 NMHH172 pKa = 7.0 RR173 pKa = 11.84 DD174 pKa = 3.31 RR175 pKa = 11.84 YY176 pKa = 8.78 QVLRR180 pKa = 11.84 KK181 pKa = 8.43 WHH183 pKa = 5.58 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.68 KK201 pKa = 9.39 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.64 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.56
IPC_protein 9.78
Toseland 10.16
ProMoST 9.867
Dawson 10.365
Bjellqvist 10.058
Wikipedia 10.555
Rodwell 10.73
Grimsley 10.438
Solomon 10.394
Lehninger 10.365
Nozaki 10.189
DTASelect 10.043
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.306
IPC_peptide 10.394
IPC2_peptide 8.916
IPC2.peptide.svr19 8.532
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1241
100
362
177.3
20.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.754 ± 1.198
2.417 ± 0.524
4.271 ± 0.618
4.754 ± 1.165
4.432 ± 0.468
4.593 ± 0.312
4.11 ± 0.641
5.157 ± 1.128
5.721 ± 0.637
7.977 ± 1.454
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.498 ± 0.656
6.285 ± 0.58
5.882 ± 0.513
5.318 ± 0.822
7.01 ± 1.018
7.655 ± 1.47
6.124 ± 0.713
5.963 ± 1.441
1.289 ± 0.115
3.787 ± 0.646
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here