Gemycircularvirus C1c
Average proteome isoelectric point is 9.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R7I4J2|A0A0R7I4J2_9VIRU Capsid protein OS=Gemycircularvirus C1c OX=1673681 GN=Cap PE=4 SV=1
MM1 pKa = 7.18 FFFVEE6 pKa = 5.19 GYY8 pKa = 8.98 VAFYY12 pKa = 11.23 VMAYY16 pKa = 7.66 AHH18 pKa = 6.23 RR19 pKa = 11.84 RR20 pKa = 11.84 FRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 FARR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 PTFARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 VPRR40 pKa = 11.84 RR41 pKa = 11.84 SYY43 pKa = 9.71 GRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 SSGRR52 pKa = 11.84 SVRR55 pKa = 11.84 SIRR58 pKa = 11.84 NIAARR63 pKa = 11.84 KK64 pKa = 9.55 CRR66 pKa = 11.84 DD67 pKa = 3.06 NMLGVPISTDD77 pKa = 3.61 FQQQNPGPLTMFGDD91 pKa = 4.18 TSHH94 pKa = 6.97 GYY96 pKa = 10.21 LFSPTARR103 pKa = 11.84 RR104 pKa = 11.84 VGSLDD109 pKa = 2.88 GSLPRR114 pKa = 11.84 GIHH117 pKa = 5.99 ANSAEE122 pKa = 4.02 RR123 pKa = 11.84 WKK125 pKa = 9.51 TRR127 pKa = 11.84 CFVKK131 pKa = 10.58 GLQEE135 pKa = 4.15 TVEE138 pKa = 4.09 LQPNNGAGWLWRR150 pKa = 11.84 RR151 pKa = 11.84 VVFSCIGLLEE161 pKa = 4.23 EE162 pKa = 5.0 FPTNSVAAPDD172 pKa = 3.55 STRR175 pKa = 11.84 GYY177 pKa = 10.92 GRR179 pKa = 11.84 AIWNMRR185 pKa = 11.84 DD186 pKa = 3.05 GSAAATPLYY195 pKa = 11.1 NEE197 pKa = 3.87 MANYY201 pKa = 9.99 LFEE204 pKa = 4.34 GTRR207 pKa = 11.84 LRR209 pKa = 11.84 DD210 pKa = 3.54 WINPFTAKK218 pKa = 10.26 LDD220 pKa = 3.75 RR221 pKa = 11.84 SIVTVHH227 pKa = 6.71 SDD229 pKa = 3.16 TTRR232 pKa = 11.84 TIQSNNPNGTYY243 pKa = 10.26 KK244 pKa = 10.18 IFKK247 pKa = 9.71 RR248 pKa = 11.84 YY249 pKa = 9.47 YY250 pKa = 7.6 PVNRR254 pKa = 11.84 GIVYY258 pKa = 10.49 ADD260 pKa = 4.25 DD261 pKa = 4.07 EE262 pKa = 4.91 SGSGSKK268 pKa = 10.59 NDD270 pKa = 3.06 AHH272 pKa = 6.36 YY273 pKa = 10.8 AAGTTKK279 pKa = 10.64 GNSGDD284 pKa = 4.59 LFVLDD289 pKa = 5.15 LFNAINDD296 pKa = 3.73 SSEE299 pKa = 3.49 NTMNFGVHH307 pKa = 6.16 ARR309 pKa = 11.84 YY310 pKa = 8.98 YY311 pKa = 7.43 WHH313 pKa = 7.21 EE314 pKa = 4.33 GSGEE318 pKa = 3.88
Molecular weight: 36.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.78
IPC_protein 10.716
Toseland 10.335
ProMoST 10.262
Dawson 10.57
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.599
Grimsley 10.672
Solomon 10.657
Lehninger 10.599
Nozaki 10.35
DTASelect 10.365
Thurlkill 10.423
EMBOSS 10.774
Sillero 10.496
Patrickios 10.014
IPC_peptide 10.657
IPC2_peptide 9.502
IPC2.peptide.svr19 8.41
Protein with the highest isoelectric point:
>tr|A0A0R7I4J2|A0A0R7I4J2_9VIRU Capsid protein OS=Gemycircularvirus C1c OX=1673681 GN=Cap PE=4 SV=1
MM1 pKa = 7.18 FFFVEE6 pKa = 5.19 GYY8 pKa = 8.98 VAFYY12 pKa = 11.23 VMAYY16 pKa = 7.66 AHH18 pKa = 6.23 RR19 pKa = 11.84 RR20 pKa = 11.84 FRR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 FARR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 PTFARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 VPRR40 pKa = 11.84 RR41 pKa = 11.84 SYY43 pKa = 9.71 GRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 SSGRR52 pKa = 11.84 SVRR55 pKa = 11.84 SIRR58 pKa = 11.84 NIAARR63 pKa = 11.84 KK64 pKa = 9.55 CRR66 pKa = 11.84 DD67 pKa = 3.06 NMLGVPISTDD77 pKa = 3.61 FQQQNPGPLTMFGDD91 pKa = 4.18 TSHH94 pKa = 6.97 GYY96 pKa = 10.21 LFSPTARR103 pKa = 11.84 RR104 pKa = 11.84 VGSLDD109 pKa = 2.88 GSLPRR114 pKa = 11.84 GIHH117 pKa = 5.99 ANSAEE122 pKa = 4.02 RR123 pKa = 11.84 WKK125 pKa = 9.51 TRR127 pKa = 11.84 CFVKK131 pKa = 10.58 GLQEE135 pKa = 4.15 TVEE138 pKa = 4.09 LQPNNGAGWLWRR150 pKa = 11.84 RR151 pKa = 11.84 VVFSCIGLLEE161 pKa = 4.23 EE162 pKa = 5.0 FPTNSVAAPDD172 pKa = 3.55 STRR175 pKa = 11.84 GYY177 pKa = 10.92 GRR179 pKa = 11.84 AIWNMRR185 pKa = 11.84 DD186 pKa = 3.05 GSAAATPLYY195 pKa = 11.1 NEE197 pKa = 3.87 MANYY201 pKa = 9.99 LFEE204 pKa = 4.34 GTRR207 pKa = 11.84 LRR209 pKa = 11.84 DD210 pKa = 3.54 WINPFTAKK218 pKa = 10.26 LDD220 pKa = 3.75 RR221 pKa = 11.84 SIVTVHH227 pKa = 6.71 SDD229 pKa = 3.16 TTRR232 pKa = 11.84 TIQSNNPNGTYY243 pKa = 10.26 KK244 pKa = 10.18 IFKK247 pKa = 9.71 RR248 pKa = 11.84 YY249 pKa = 9.47 YY250 pKa = 7.6 PVNRR254 pKa = 11.84 GIVYY258 pKa = 10.49 ADD260 pKa = 4.25 DD261 pKa = 4.07 EE262 pKa = 4.91 SGSGSKK268 pKa = 10.59 NDD270 pKa = 3.06 AHH272 pKa = 6.36 YY273 pKa = 10.8 AAGTTKK279 pKa = 10.64 GNSGDD284 pKa = 4.59 LFVLDD289 pKa = 5.15 LFNAINDD296 pKa = 3.73 SSEE299 pKa = 3.49 NTMNFGVHH307 pKa = 6.16 ARR309 pKa = 11.84 YY310 pKa = 8.98 YY311 pKa = 7.43 WHH313 pKa = 7.21 EE314 pKa = 4.33 GSGEE318 pKa = 3.88
Molecular weight: 36.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.78
IPC_protein 10.716
Toseland 10.335
ProMoST 10.262
Dawson 10.57
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.599
Grimsley 10.672
Solomon 10.657
Lehninger 10.599
Nozaki 10.35
DTASelect 10.365
Thurlkill 10.423
EMBOSS 10.774
Sillero 10.496
Patrickios 10.014
IPC_peptide 10.657
IPC2_peptide 9.502
IPC2.peptide.svr19 8.41
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
318
318
318
318.0
36.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.862 ± 0.0
0.943 ± 0.0
4.717 ± 0.0
3.774 ± 0.0
5.975 ± 0.0
8.805 ± 0.0
2.201 ± 0.0
3.774 ± 0.0
2.516 ± 0.0
5.346 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.201 ± 0.0
6.604 ± 0.0
4.403 ± 0.0
1.887 ± 0.0
12.579 ± 0.0
7.862 ± 0.0
6.289 ± 0.0
5.66 ± 0.0
1.887 ± 0.0
4.717 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here