Streptococcus phage D1811

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U7VK08|A0A2U7VK08_9CAUD Helicase OS=Streptococcus phage D1811 OX=2108111 GN=D1811_035 PE=4 SV=1
MM1 pKa = 6.82ITKK4 pKa = 9.33EE5 pKa = 3.87QLKK8 pKa = 9.98EE9 pKa = 3.95YY10 pKa = 10.04YY11 pKa = 9.76SEE13 pKa = 4.08HH14 pKa = 8.22LEE16 pKa = 4.53EE17 pKa = 4.21LVEE20 pKa = 4.04WADD23 pKa = 5.14DD24 pKa = 3.53INKK27 pKa = 9.26ICLFAYY33 pKa = 10.49LDD35 pKa = 3.99EE36 pKa = 6.43DD37 pKa = 4.32DD38 pKa = 5.79NLYY41 pKa = 11.03CGINQLSYY49 pKa = 8.92TQFRR53 pKa = 11.84VPIQAEE59 pKa = 4.28VTVDD63 pKa = 4.08DD64 pKa = 4.11DD65 pKa = 3.64WNYY68 pKa = 11.7DD69 pKa = 3.57FFKK72 pKa = 11.27NPAAYY77 pKa = 10.01DD78 pKa = 3.45GWDD81 pKa = 3.4EE82 pKa = 4.11TLEE85 pKa = 4.07EE86 pKa = 4.29MLEE89 pKa = 4.12EE90 pKa = 5.03LNDD93 pKa = 3.6

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U7VK06|A0A2U7VK06_9CAUD Uncharacterized protein OS=Streptococcus phage D1811 OX=2108111 GN=D1811_027 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84VRR5 pKa = 11.84YY6 pKa = 9.32LPSDD10 pKa = 3.79FRR12 pKa = 11.84YY13 pKa = 9.99KK14 pKa = 10.95ADD16 pKa = 3.48FGTYY20 pKa = 9.38QSTPNKK26 pKa = 8.16FTGVSVPKK34 pKa = 10.14FVKK37 pKa = 10.38QFTLHH42 pKa = 5.9YY43 pKa = 9.16KK44 pKa = 9.46PHH46 pKa = 6.1TRR48 pKa = 11.84TLNQEE53 pKa = 3.83YY54 pKa = 10.01LAQQNGEE61 pKa = 3.75SDD63 pKa = 3.27TRR65 pKa = 11.84VIVIRR70 pKa = 11.84HH71 pKa = 4.8NAKK74 pKa = 9.68VLEE77 pKa = 4.47GQVVTVNGTQYY88 pKa = 11.1DD89 pKa = 3.79IVRR92 pKa = 11.84ISPDD96 pKa = 3.02EE97 pKa = 3.9NFGFNHH103 pKa = 6.62YY104 pKa = 11.07DD105 pKa = 3.15FLTLRR110 pKa = 11.84KK111 pKa = 8.91HH112 pKa = 6.08KK113 pKa = 10.69KK114 pKa = 9.55VGG116 pKa = 3.25

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

10371

29

1591

259.3

29.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.75 ± 0.551

0.53 ± 0.152

6.547 ± 0.321

6.431 ± 0.536

4.3 ± 0.199

6.981 ± 0.546

1.408 ± 0.147

6.576 ± 0.264

8.177 ± 0.555

7.608 ± 0.401

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.245

6.451 ± 0.264

3.057 ± 0.191

4.387 ± 0.226

4.011 ± 0.307

6.364 ± 0.314

6.489 ± 0.37

5.863 ± 0.223

1.62 ± 0.15

4.088 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski