Streptococcus phage D1811
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U7VK08|A0A2U7VK08_9CAUD Helicase OS=Streptococcus phage D1811 OX=2108111 GN=D1811_035 PE=4 SV=1
MM1 pKa = 6.82 ITKK4 pKa = 9.33 EE5 pKa = 3.87 QLKK8 pKa = 9.98 EE9 pKa = 3.95 YY10 pKa = 10.04 YY11 pKa = 9.76 SEE13 pKa = 4.08 HH14 pKa = 8.22 LEE16 pKa = 4.53 EE17 pKa = 4.21 LVEE20 pKa = 4.04 WADD23 pKa = 5.14 DD24 pKa = 3.53 INKK27 pKa = 9.26 ICLFAYY33 pKa = 10.49 LDD35 pKa = 3.99 EE36 pKa = 6.43 DD37 pKa = 4.32 DD38 pKa = 5.79 NLYY41 pKa = 11.03 CGINQLSYY49 pKa = 8.92 TQFRR53 pKa = 11.84 VPIQAEE59 pKa = 4.28 VTVDD63 pKa = 4.08 DD64 pKa = 4.11 DD65 pKa = 3.64 WNYY68 pKa = 11.7 DD69 pKa = 3.57 FFKK72 pKa = 11.27 NPAAYY77 pKa = 10.01 DD78 pKa = 3.45 GWDD81 pKa = 3.4 EE82 pKa = 4.11 TLEE85 pKa = 4.07 EE86 pKa = 4.29 MLEE89 pKa = 4.12 EE90 pKa = 5.03 LNDD93 pKa = 3.6
Molecular weight: 11.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.427
ProMoST 3.732
Dawson 3.605
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.452
Grimsley 3.338
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.91
Thurlkill 3.478
EMBOSS 3.541
Sillero 3.745
Patrickios 0.362
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A2U7VK06|A0A2U7VK06_9CAUD Uncharacterized protein OS=Streptococcus phage D1811 OX=2108111 GN=D1811_027 PE=4 SV=1
MM1 pKa = 7.36 ARR3 pKa = 11.84 VRR5 pKa = 11.84 YY6 pKa = 9.32 LPSDD10 pKa = 3.79 FRR12 pKa = 11.84 YY13 pKa = 9.99 KK14 pKa = 10.95 ADD16 pKa = 3.48 FGTYY20 pKa = 9.38 QSTPNKK26 pKa = 8.16 FTGVSVPKK34 pKa = 10.14 FVKK37 pKa = 10.38 QFTLHH42 pKa = 5.9 YY43 pKa = 9.16 KK44 pKa = 9.46 PHH46 pKa = 6.1 TRR48 pKa = 11.84 TLNQEE53 pKa = 3.83 YY54 pKa = 10.01 LAQQNGEE61 pKa = 3.75 SDD63 pKa = 3.27 TRR65 pKa = 11.84 VIVIRR70 pKa = 11.84 HH71 pKa = 4.8 NAKK74 pKa = 9.68 VLEE77 pKa = 4.47 GQVVTVNGTQYY88 pKa = 11.1 DD89 pKa = 3.79 IVRR92 pKa = 11.84 ISPDD96 pKa = 3.02 EE97 pKa = 3.9 NFGFNHH103 pKa = 6.62 YY104 pKa = 11.07 DD105 pKa = 3.15 FLTLRR110 pKa = 11.84 KK111 pKa = 8.91 HH112 pKa = 6.08 KK113 pKa = 10.69 KK114 pKa = 9.55 VGG116 pKa = 3.25
Molecular weight: 13.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.144
IPC2_protein 9.341
IPC_protein 9.37
Toseland 9.823
ProMoST 9.589
Dawson 10.087
Bjellqvist 9.78
Wikipedia 10.277
Rodwell 10.394
Grimsley 10.175
Solomon 10.116
Lehninger 10.072
Nozaki 9.78
DTASelect 9.78
Thurlkill 9.911
EMBOSS 10.233
Sillero 9.984
Patrickios 7.6
IPC_peptide 10.116
IPC2_peptide 8.346
IPC2.peptide.svr19 8.369
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
10371
29
1591
259.3
29.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.75 ± 0.551
0.53 ± 0.152
6.547 ± 0.321
6.431 ± 0.536
4.3 ± 0.199
6.981 ± 0.546
1.408 ± 0.147
6.576 ± 0.264
8.177 ± 0.555
7.608 ± 0.401
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.362 ± 0.245
6.451 ± 0.264
3.057 ± 0.191
4.387 ± 0.226
4.011 ± 0.307
6.364 ± 0.314
6.489 ± 0.37
5.863 ± 0.223
1.62 ± 0.15
4.088 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here