Mycobacterium phage Aminay

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KV25|A0A345KV25_9CAUD Helix-turn-helix DNA binding domain protein OS=Mycobacterium phage Aminay OX=2250291 GN=39 PE=4 SV=1
MM1 pKa = 7.11TAGDD5 pKa = 4.81GIRR8 pKa = 11.84AAIEE12 pKa = 3.92AMLDD16 pKa = 3.55EE17 pKa = 5.57SGDD20 pKa = 4.03GYY22 pKa = 11.29QLGQLVIVMSLEE34 pKa = 4.52HH35 pKa = 6.77INADD39 pKa = 3.64GQIEE43 pKa = 4.36TVPWLWAPPDD53 pKa = 3.46QAEE56 pKa = 4.11WMTDD60 pKa = 3.21GLLSTALDD68 pKa = 3.7LRR70 pKa = 11.84ATADD74 pKa = 3.29EE75 pKa = 5.27HH76 pKa = 7.72YY77 pKa = 10.78DD78 pKa = 3.27

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KV90|A0A345KV90_9CAUD Membrane protein OS=Mycobacterium phage Aminay OX=2250291 GN=59 PE=4 SV=1
MM1 pKa = 7.1HH2 pKa = 8.0RR3 pKa = 11.84SRR5 pKa = 11.84GSRR8 pKa = 11.84PFLLAICRR16 pKa = 11.84TCRR19 pKa = 11.84SVARR23 pKa = 11.84VVKK26 pKa = 10.31LCNIDD31 pKa = 3.29TCQRR35 pKa = 11.84PVLGRR40 pKa = 11.84GMCPSHH46 pKa = 6.21YY47 pKa = 9.84NAWNRR52 pKa = 11.84AQRR55 pKa = 11.84RR56 pKa = 11.84HH57 pKa = 5.96TIVCAEE63 pKa = 4.51CGQTAQVDD71 pKa = 3.51RR72 pKa = 11.84SDD74 pKa = 5.99RR75 pKa = 11.84ILCGNACRR83 pKa = 11.84MEE85 pKa = 4.35RR86 pKa = 11.84ARR88 pKa = 11.84KK89 pKa = 8.54AAAAANRR96 pKa = 11.84AAGWAARR103 pKa = 11.84AARR106 pKa = 11.84SQLVPYY112 pKa = 8.95TGPPYY117 pKa = 9.32VAPVRR122 pKa = 11.84TVQGKK127 pKa = 8.07GLWTSGQCRR136 pKa = 11.84VCGAWFTSQHH146 pKa = 6.48LDD148 pKa = 3.43VTCSPEE154 pKa = 3.82CWHH157 pKa = 6.2QRR159 pKa = 11.84MAEE162 pKa = 3.92MTRR165 pKa = 11.84EE166 pKa = 3.8HH167 pKa = 6.05KK168 pKa = 9.77QRR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84ARR174 pKa = 11.84KK175 pKa = 8.11RR176 pKa = 11.84NAYY179 pKa = 7.88VAPVNRR185 pKa = 11.84RR186 pKa = 11.84KK187 pKa = 10.34VFEE190 pKa = 4.35ADD192 pKa = 3.34GYY194 pKa = 10.0RR195 pKa = 11.84CHH197 pKa = 6.66ICRR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 11.84CDD204 pKa = 3.3KK205 pKa = 10.42TKK207 pKa = 9.98PAPHH211 pKa = 6.91PRR213 pKa = 11.84SPTIDD218 pKa = 3.23HH219 pKa = 6.9VIPLAAGGTHH229 pKa = 6.44EE230 pKa = 4.74PANCRR235 pKa = 11.84TACFQCNAVKK245 pKa = 10.45GDD247 pKa = 3.74RR248 pKa = 11.84GSGEE252 pKa = 3.96QFALAFF258 pKa = 4.36

Molecular weight: 28.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

19027

27

1433

181.2

19.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.444 ± 0.459

1.056 ± 0.162

6.312 ± 0.279

5.208 ± 0.331

2.575 ± 0.136

9.124 ± 0.573

1.987 ± 0.186

4.283 ± 0.205

3.222 ± 0.209

7.994 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.118 ± 0.103

2.817 ± 0.163

5.655 ± 0.214

3.343 ± 0.202

7.495 ± 0.465

5.161 ± 0.247

6.596 ± 0.259

7.079 ± 0.217

2.129 ± 0.119

2.402 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski