Monosporascus sp. CRB-8-3

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4Z236|A0A4Q4Z236_9PEZI J domain-containing protein OS=Monosporascus sp. CRB-8-3 OX=2211644 GN=DL769_002219 PE=4 SV=1
MM1 pKa = 7.59YY2 pKa = 7.97YY3 pKa = 7.79TTASFLALLGLASCQKK19 pKa = 9.52LHH21 pKa = 5.79VVSVSGEE28 pKa = 4.3GEE30 pKa = 3.81WALKK34 pKa = 9.78FMPDD38 pKa = 3.65NIKK41 pKa = 10.55AAAGDD46 pKa = 3.78MIQFQFRR53 pKa = 11.84AGNHH57 pKa = 5.33SVVQSNFDD65 pKa = 3.78NPCEE69 pKa = 4.28PIKK72 pKa = 10.41MHH74 pKa = 6.86TDD76 pKa = 3.06TEE78 pKa = 4.68GFYY81 pKa = 10.77SGYY84 pKa = 10.18QPVAASEE91 pKa = 4.18EE92 pKa = 4.15MDD94 pKa = 4.22MIPTYY99 pKa = 10.72TIMVQDD105 pKa = 5.08DD106 pKa = 3.86KK107 pKa = 11.51PMWMYY112 pKa = 10.81CSQGQHH118 pKa = 5.97CQNGMTMVVNEE129 pKa = 3.92NTAANSSRR137 pKa = 11.84SLDD140 pKa = 3.65EE141 pKa = 3.78YY142 pKa = 11.2RR143 pKa = 11.84KK144 pKa = 9.88LAAEE148 pKa = 4.6APDD151 pKa = 3.92NLAGDD156 pKa = 3.82VPGAGGEE163 pKa = 4.32TGTTPEE169 pKa = 4.15PTEE172 pKa = 4.55PGDD175 pKa = 5.29DD176 pKa = 3.55EE177 pKa = 4.42TTAPGDD183 pKa = 3.81EE184 pKa = 4.5EE185 pKa = 4.76TPATPGGDD193 pKa = 3.4DD194 pKa = 3.58TTDD197 pKa = 3.92DD198 pKa = 4.37GASDD202 pKa = 4.97DD203 pKa = 5.46APTDD207 pKa = 3.7DD208 pKa = 4.7DD209 pKa = 4.45TTVGTPGEE217 pKa = 4.58DD218 pKa = 3.31TTSTPGSDD226 pKa = 3.17ATPTGTAGGVASGTEE241 pKa = 3.98AADD244 pKa = 3.89GASTSIAQVAGGSMVTVTSPMGFFALAAAFFVLL277 pKa = 4.98

Molecular weight: 28.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4YZM1|A0A4Q4YZM1_9PEZI FAA_hydrolase domain-containing protein OS=Monosporascus sp. CRB-8-3 OX=2211644 GN=DL769_002359 PE=3 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84QYY4 pKa = 11.51HH5 pKa = 7.55DD6 pKa = 3.13ITEE9 pKa = 4.5LEE11 pKa = 3.9RR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84MEE18 pKa = 3.8EE19 pKa = 3.4RR20 pKa = 11.84RR21 pKa = 11.84GLYY24 pKa = 9.47QRR26 pKa = 11.84AASFKK31 pKa = 9.01AHH33 pKa = 6.46AEE35 pKa = 3.96AMEE38 pKa = 4.31VAQQRR43 pKa = 11.84AKK45 pKa = 10.18IVRR48 pKa = 11.84PLEE51 pKa = 4.2SLPNPAVPAPAEE63 pKa = 3.94AAAGLGRR70 pKa = 11.84LAFLPNEE77 pKa = 4.16VVMMVLQHH85 pKa = 5.38CTVRR89 pKa = 11.84TLFRR93 pKa = 11.84LQRR96 pKa = 11.84VNRR99 pKa = 11.84ATGMMVRR106 pKa = 11.84LLPNFSYY113 pKa = 9.44VTNTAKK119 pKa = 10.66GMLEE123 pKa = 3.71RR124 pKa = 11.84AIMKK128 pKa = 10.25RR129 pKa = 11.84LTATLTFTGLRR140 pKa = 11.84HH141 pKa = 6.38LLMSSTCEE149 pKa = 3.71NCGKK153 pKa = 10.29GGCSFQITKK162 pKa = 10.64LGVLCYY168 pKa = 9.86EE169 pKa = 4.49CRR171 pKa = 11.84APPRR175 pKa = 11.84TWIRR179 pKa = 11.84GAKK182 pKa = 9.39GKK184 pKa = 10.8AKK186 pKa = 10.42VPPNN190 pKa = 3.44

Molecular weight: 21.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11773

0

11773

5704079

66

8401

484.5

53.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.184 ± 0.02

1.173 ± 0.008

5.91 ± 0.017

6.347 ± 0.024

3.552 ± 0.014

7.352 ± 0.021

2.333 ± 0.009

4.535 ± 0.017

4.584 ± 0.02

8.74 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.106 ± 0.008

3.526 ± 0.012

6.217 ± 0.026

3.841 ± 0.015

6.579 ± 0.022

7.821 ± 0.026

5.778 ± 0.018

6.22 ± 0.016

1.47 ± 0.008

2.731 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski