Roseomonas stagni DSM 19981

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas; Roseomonas stagni

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5902 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3XKI8|A0A1I3XKI8_9PROT Uncharacterized protein OS=Roseomonas stagni DSM 19981 OX=1123062 GN=SAMN02745775_101406 PE=4 SV=1
MM1 pKa = 7.21SQTLSGDD8 pKa = 3.29NVTDD12 pKa = 5.64SIFTWTFTANSGDD25 pKa = 3.47SWGGTLVDD33 pKa = 4.27DD34 pKa = 4.41GTRR37 pKa = 11.84YY38 pKa = 10.06VVGSILNTATGRR50 pKa = 11.84YY51 pKa = 7.22TIVAAAPQGVDD62 pKa = 3.4LSAFGMNEE70 pKa = 3.1GWIAVSWYY78 pKa = 9.7RR79 pKa = 11.84DD80 pKa = 3.13ASGVFMVTRR89 pKa = 11.84NGAGTASGTAGLGSEE104 pKa = 5.65VDD106 pKa = 3.74AAWNGTAWDD115 pKa = 3.9SFGLGGSDD123 pKa = 3.75QADD126 pKa = 3.87PGDD129 pKa = 4.0LADD132 pKa = 6.36SLFTWTFTADD142 pKa = 3.59SGDD145 pKa = 3.45IMQGTLLDD153 pKa = 4.18DD154 pKa = 3.68TRR156 pKa = 11.84NWDD159 pKa = 3.46PGDD162 pKa = 3.5TRR164 pKa = 11.84RR165 pKa = 11.84TAFGTYY171 pKa = 9.96RR172 pKa = 11.84IDD174 pKa = 3.41TEE176 pKa = 3.97IPYY179 pKa = 10.8GRR181 pKa = 11.84DD182 pKa = 2.89LGSAGVEE189 pKa = 3.99GTITIVSYY197 pKa = 11.21TDD199 pKa = 3.76FFADD203 pKa = 3.04IQFTLEE209 pKa = 3.96TGASGPAGYY218 pKa = 10.6GGFGSEE224 pKa = 4.96WDD226 pKa = 3.98RR227 pKa = 11.84AWTGTAWMPVGQGGALQADD246 pKa = 4.68RR247 pKa = 11.84LPDD250 pKa = 3.41RR251 pKa = 11.84VFSWRR256 pKa = 11.84FTADD260 pKa = 2.85NGDD263 pKa = 3.64QYY265 pKa = 11.05VGSMVGHH272 pKa = 6.41SSALTTGDD280 pKa = 4.53RR281 pKa = 11.84IEE283 pKa = 4.6TDD285 pKa = 2.77HH286 pKa = 6.11GHH288 pKa = 6.51YY289 pKa = 10.59DD290 pKa = 3.25ILQEE294 pKa = 4.06NAYY297 pKa = 9.97SGPASAQGMVWVLRR311 pKa = 11.84YY312 pKa = 9.85YY313 pKa = 10.6DD314 pKa = 4.01ASLDD318 pKa = 3.54TWMTTYY324 pKa = 10.6QNSVATTASGTRR336 pKa = 11.84GLGSEE341 pKa = 4.83YY342 pKa = 10.7DD343 pKa = 3.98YY344 pKa = 11.89AWDD347 pKa = 3.67GDD349 pKa = 3.55EE350 pKa = 4.58WDD352 pKa = 4.55DD353 pKa = 4.0FGLGGVHH360 pKa = 7.0LASIEE365 pKa = 4.07RR366 pKa = 11.84NNLFGWTFTANSGDD380 pKa = 4.67RR381 pKa = 11.84YY382 pKa = 8.85TGWLIAADD390 pKa = 3.91DD391 pKa = 4.63AYY393 pKa = 10.95AAGDD397 pKa = 3.98TVAGAQGRR405 pKa = 11.84YY406 pKa = 8.81QITYY410 pKa = 8.17EE411 pKa = 4.29GTWGGAAGAGTIYY424 pKa = 7.44TTSYY428 pKa = 10.95SDD430 pKa = 3.25AGSGRR435 pKa = 11.84SFDD438 pKa = 3.52TYY440 pKa = 10.49RR441 pKa = 11.84WGTVGGQPSGRR452 pKa = 11.84AGFGSEE458 pKa = 5.35YY459 pKa = 10.64DD460 pKa = 4.06YY461 pKa = 11.3IWNGSEE467 pKa = 3.48WDD469 pKa = 3.74DD470 pKa = 4.13FGVGGVHH477 pKa = 5.76QANYY481 pKa = 9.17TINTLHH487 pKa = 7.13AFTFTANSGDD497 pKa = 4.63RR498 pKa = 11.84YY499 pKa = 8.92TGWLVEE505 pKa = 4.25DD506 pKa = 3.66SARR509 pKa = 11.84YY510 pKa = 9.1AVGNMIATSQGSYY523 pKa = 10.15RR524 pKa = 11.84ITWEE528 pKa = 4.1GDD530 pKa = 2.49WGGTQGRR537 pKa = 11.84GSIWTDD543 pKa = 2.91SYY545 pKa = 11.71FDD547 pKa = 3.74AASGRR552 pKa = 11.84SFGTYY557 pKa = 7.03TWTAGGGRR565 pKa = 11.84PSGQAGFGSEE575 pKa = 4.42YY576 pKa = 10.75DD577 pKa = 4.14YY578 pKa = 11.29IWTGTEE584 pKa = 3.28WDD586 pKa = 3.9DD587 pKa = 4.11FGVGGVHH594 pKa = 6.56QSTFTINTLHH604 pKa = 7.04AFTFTASSGDD614 pKa = 3.87RR615 pKa = 11.84YY616 pKa = 10.78SGWLIEE622 pKa = 4.54DD623 pKa = 3.46STRR626 pKa = 11.84YY627 pKa = 9.91AVGDD631 pKa = 4.22TITTSQGSYY640 pKa = 10.36RR641 pKa = 11.84ITFEE645 pKa = 4.15GDD647 pKa = 2.26WGGTQGRR654 pKa = 11.84GSIWTDD660 pKa = 2.87SYY662 pKa = 11.77FDD664 pKa = 3.88AGTGRR669 pKa = 11.84SFATYY674 pKa = 9.57FWGTLGAQPSGRR686 pKa = 11.84SGFGSEE692 pKa = 5.29YY693 pKa = 10.75DD694 pKa = 4.09YY695 pKa = 11.4IWDD698 pKa = 3.64GDD700 pKa = 3.47EE701 pKa = 3.88WDD703 pKa = 4.25DD704 pKa = 4.18FGVGGIHH711 pKa = 5.96QANVTVNTLFGWTFTANSGDD731 pKa = 4.67RR732 pKa = 11.84YY733 pKa = 8.87TGWLIQDD740 pKa = 3.52DD741 pKa = 3.64TRR743 pKa = 11.84YY744 pKa = 10.59AVGDD748 pKa = 4.27TIATSQGSYY757 pKa = 10.35RR758 pKa = 11.84ITHH761 pKa = 6.29EE762 pKa = 4.54GPWGGTAGKK771 pKa = 8.11NTIWTTSYY779 pKa = 11.08FDD781 pKa = 3.8AGSNQTFEE789 pKa = 4.39TYY791 pKa = 9.3FWNTIGGQPSGRR803 pKa = 11.84GGFGSEE809 pKa = 4.17YY810 pKa = 10.52DD811 pKa = 3.97YY812 pKa = 11.88VWDD815 pKa = 3.82GRR817 pKa = 11.84EE818 pKa = 3.64WDD820 pKa = 3.86DD821 pKa = 4.11FGVGGVHH828 pKa = 5.1QVRR831 pKa = 11.84VEE833 pKa = 4.01RR834 pKa = 11.84AVMVSWIFRR843 pKa = 11.84ATSGDD848 pKa = 3.88AYY850 pKa = 9.97TGLLLEE856 pKa = 4.98DD857 pKa = 4.49AEE859 pKa = 4.95TYY861 pKa = 11.11APGDD865 pKa = 3.65TIVRR869 pKa = 11.84PNGTYY874 pKa = 10.09TIQSEE879 pKa = 4.46TDD881 pKa = 3.26TNRR884 pKa = 11.84TDD886 pKa = 3.52YY887 pKa = 11.49GIGTIWTAAYY897 pKa = 10.02YY898 pKa = 10.29DD899 pKa = 4.15AGSATWLTTHH909 pKa = 6.82YY910 pKa = 10.68YY911 pKa = 10.69GFYY914 pKa = 10.66DD915 pKa = 4.18GLPSGRR921 pKa = 11.84SGFGSEE927 pKa = 5.84YY928 pKa = 11.1DD929 pKa = 3.86FAWDD933 pKa = 3.24GDD935 pKa = 3.55EE936 pKa = 3.99WDD938 pKa = 4.37DD939 pKa = 4.25FGVAGLHH946 pKa = 5.23QADD949 pKa = 4.29VEE951 pKa = 4.31RR952 pKa = 11.84ASAPEE957 pKa = 3.72VLGG960 pKa = 3.95

Molecular weight: 103.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4ABG7|A0A1I4ABG7_9PROT Phosphoenolpyruvate-protein phosphotransferase OS=Roseomonas stagni DSM 19981 OX=1123062 GN=SAMN02745775_103291 PE=3 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84SLSRR6 pKa = 11.84LPLAATLLLIGAVPAFAQGSSGTVTSTAPVATTRR40 pKa = 11.84PAVTAPAAPTQRR52 pKa = 11.84PTQAATPANPGGSAQATAPAARR74 pKa = 11.84PATQGQQAQPATPAVPQAPRR94 pKa = 11.84ATNN97 pKa = 3.19

Molecular weight: 9.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5902

0

5902

1962004

8

7448

332.4

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.48 ± 0.059

0.784 ± 0.011

5.323 ± 0.038

5.102 ± 0.03

3.246 ± 0.021

9.502 ± 0.054

1.931 ± 0.017

4.282 ± 0.024

1.808 ± 0.023

10.685 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.017

2.034 ± 0.018

6.256 ± 0.038

2.905 ± 0.019

8.192 ± 0.039

4.324 ± 0.033

5.198 ± 0.038

7.425 ± 0.028

1.432 ± 0.014

1.608 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski