Planococcus salinus
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3216 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M8P7P5|A0A3M8P7P5_9BACL Class I SAM-dependent methyltransferase OS=Planococcus salinus OX=1848460 GN=EEX84_07040 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.51 NSFKK6 pKa = 11.13 VIVLFLCSILILSGCAGFQTSNSDD30 pKa = 3.34 EE31 pKa = 4.44 GNTNEE36 pKa = 4.98 DD37 pKa = 3.19 GKK39 pKa = 9.15 TVVDD43 pKa = 6.07 FWSFWGSEE51 pKa = 3.89 LRR53 pKa = 11.84 RR54 pKa = 11.84 PIIDD58 pKa = 4.66 KK59 pKa = 10.64 IVADD63 pKa = 5.32 FNASQDD69 pKa = 3.87 EE70 pKa = 4.5 IEE72 pKa = 4.52 VKK74 pKa = 8.92 HH75 pKa = 6.1 TYY77 pKa = 10.39 SPWGDD82 pKa = 2.39 IWTKK86 pKa = 10.4 EE87 pKa = 3.83 LAAIAAGNPPDD98 pKa = 5.08 VVINDD103 pKa = 3.42 INATAIRR110 pKa = 11.84 GQEE113 pKa = 3.85 NQAMNLSEE121 pKa = 5.21 FYY123 pKa = 10.78 DD124 pKa = 3.73 DD125 pKa = 4.56 SFSEE129 pKa = 4.15 KK130 pKa = 10.25 FYY132 pKa = 10.82 PEE134 pKa = 3.91 LWNATLYY141 pKa = 10.99 EE142 pKa = 4.5 GDD144 pKa = 3.69 SYY146 pKa = 11.53 GIPFNTDD153 pKa = 2.29 TRR155 pKa = 11.84 VLYY158 pKa = 10.02 YY159 pKa = 10.9 NKK161 pKa = 10.36 DD162 pKa = 3.08 AFEE165 pKa = 4.24 EE166 pKa = 4.32 VGLDD170 pKa = 3.62 PEE172 pKa = 5.19 DD173 pKa = 4.58 PPEE176 pKa = 3.91 TWEE179 pKa = 4.01 EE180 pKa = 4.13 LEE182 pKa = 4.39 EE183 pKa = 4.07 YY184 pKa = 10.1 AAKK187 pKa = 10.6 LDD189 pKa = 3.83 VKK191 pKa = 10.89 NGEE194 pKa = 4.17 SYY196 pKa = 11.31 DD197 pKa = 3.68 RR198 pKa = 11.84 LGFYY202 pKa = 9.8 PLWGVGYY209 pKa = 9.73 DD210 pKa = 3.47 VWLLNANGEE219 pKa = 4.42 NYY221 pKa = 10.22 FGADD225 pKa = 3.34 NNVDD229 pKa = 3.65 TVNVNTEE236 pKa = 4.13 TNVEE240 pKa = 4.0 VLNWLKK246 pKa = 10.49 SWRR249 pKa = 11.84 DD250 pKa = 3.51 RR251 pKa = 11.84 IGEE254 pKa = 4.17 DD255 pKa = 3.93 VVNSYY260 pKa = 9.05 QAQIDD265 pKa = 4.15 SQQGNPFFSGDD276 pKa = 3.37 LAMLVQQPTFYY287 pKa = 10.45 TQIRR291 pKa = 11.84 DD292 pKa = 3.57 YY293 pKa = 11.8 AEE295 pKa = 4.28 DD296 pKa = 4.04 LNFGVAQLPEE306 pKa = 4.17 YY307 pKa = 10.58 EE308 pKa = 4.82 PGNGHH313 pKa = 5.81 TSWGGGFVAEE323 pKa = 4.38 IPEE326 pKa = 4.59 GSSDD330 pKa = 5.05 PEE332 pKa = 3.9 AAWAFIEE339 pKa = 4.48 YY340 pKa = 8.7 LTGPEE345 pKa = 3.93 AQEE348 pKa = 3.67 YY349 pKa = 8.05 WAVQNFDD356 pKa = 3.02 NVANIEE362 pKa = 4.05 AAEE365 pKa = 4.11 AAAQSDD371 pKa = 4.21 EE372 pKa = 4.17 FTEE375 pKa = 4.46 EE376 pKa = 3.91 GQMVYY381 pKa = 10.43 QMAVDD386 pKa = 3.64 SMEE389 pKa = 4.18 NTLLTPAPVEE399 pKa = 3.86 APGFYY404 pKa = 10.6 NYY406 pKa = 9.96 INPKK410 pKa = 9.44 VDD412 pKa = 3.32 EE413 pKa = 4.77 FFLGSLTAEE422 pKa = 4.11 EE423 pKa = 4.97 ALAQAQADD431 pKa = 3.92 VEE433 pKa = 4.46 ALIEE437 pKa = 4.49 SNQQ440 pKa = 3.22
Molecular weight: 49.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.541
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.605
Rodwell 3.567
Grimsley 3.452
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.859
Patrickios 1.049
IPC_peptide 3.706
IPC2_peptide 3.846
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A3M8P8K1|A0A3M8P8K1_9BACL DNA replication and repair protein RecF OS=Planococcus salinus OX=1848460 GN=recF PE=3 SV=1
MM1 pKa = 7.67 SLRR4 pKa = 11.84 TYY6 pKa = 10.39 QPNTRR11 pKa = 11.84 KK12 pKa = 9.66 HH13 pKa = 5.87 SKK15 pKa = 8.83 VHH17 pKa = 5.7 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 9.71 NGRR29 pKa = 11.84 RR30 pKa = 11.84 VIAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.8 GRR40 pKa = 11.84 KK41 pKa = 8.75 VLSAA45 pKa = 4.05
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3216
0
3216
962098
37
1528
299.2
33.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.111 ± 0.045
0.584 ± 0.013
5.204 ± 0.037
7.989 ± 0.057
4.523 ± 0.035
7.247 ± 0.037
2.042 ± 0.022
7.054 ± 0.044
5.944 ± 0.046
9.717 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.917 ± 0.021
3.777 ± 0.026
3.828 ± 0.026
3.765 ± 0.029
4.312 ± 0.031
5.901 ± 0.028
5.508 ± 0.026
7.327 ± 0.029
1.023 ± 0.017
3.227 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here