Streptococcus satellite phage Javan171

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZG30|A0A4D5ZG30_9VIRU ArpU family encoded transcriptional regulator OS=Streptococcus satellite phage Javan171 OX=2558554 GN=JavanS171_0021 PE=4 SV=1
MM1 pKa = 7.41QEE3 pKa = 4.4LNLTPTQTLILFFVLSLLGLLLSLSKK29 pKa = 10.98SLIDD33 pKa = 3.72IDD35 pKa = 5.29LPEE38 pKa = 4.75DD39 pKa = 3.72TQIPKK44 pKa = 9.94PPKK47 pKa = 7.6NANYY51 pKa = 9.91GAYY54 pKa = 9.28IQAQNHH60 pKa = 5.84YY61 pKa = 9.74YY62 pKa = 10.34NN63 pKa = 4.08

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZG21|A0A4D5ZG21_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan171 OX=2558554 GN=JavanS171_0011 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.26IKK4 pKa = 9.69EE5 pKa = 4.22HH6 pKa = 6.26KK7 pKa = 9.48KK8 pKa = 9.51KK9 pKa = 10.71NGTIVYY15 pKa = 9.02RR16 pKa = 11.84ASIYY20 pKa = 10.8LGIDD24 pKa = 2.83QMTGKK29 pKa = 10.05RR30 pKa = 11.84VKK32 pKa = 9.97TSITGRR38 pKa = 11.84TRR40 pKa = 11.84KK41 pKa = 9.11EE42 pKa = 3.7VNQKK46 pKa = 10.3AKK48 pKa = 10.27HH49 pKa = 5.39AQLDD53 pKa = 3.99FLSNGSTIKK62 pKa = 10.43RR63 pKa = 11.84KK64 pKa = 9.88VVIKK68 pKa = 8.87TFKK71 pKa = 10.26EE72 pKa = 4.46LSHH75 pKa = 6.68LWLEE79 pKa = 4.6TYY81 pKa = 10.71KK82 pKa = 10.43LTVKK86 pKa = 10.0PQTYY90 pKa = 9.55DD91 pKa = 3.09ATVTRR96 pKa = 11.84LNRR99 pKa = 11.84HH100 pKa = 5.38IMPTLGNMKK109 pKa = 9.51VDD111 pKa = 4.87KK112 pKa = 9.89ITASDD117 pKa = 3.12IQMLINRR124 pKa = 11.84LSKK127 pKa = 11.11YY128 pKa = 8.49YY129 pKa = 11.04VNYY132 pKa = 8.12TAVRR136 pKa = 11.84SVIRR140 pKa = 11.84KK141 pKa = 8.39VLQQGVLLGLIDD153 pKa = 3.95YY154 pKa = 10.62NSARR158 pKa = 11.84DD159 pKa = 3.44IILPRR164 pKa = 11.84KK165 pKa = 8.91QPNAKK170 pKa = 9.88KK171 pKa = 10.0KK172 pKa = 10.78VKK174 pKa = 10.55FIDD177 pKa = 3.98ASDD180 pKa = 3.57LKK182 pKa = 11.27SFLEE186 pKa = 4.04HH187 pKa = 7.4LEE189 pKa = 4.21TSQHH193 pKa = 5.32KK194 pKa = 10.23RR195 pKa = 11.84YY196 pKa = 10.13NLYY199 pKa = 10.34FDD201 pKa = 4.37AVLYY205 pKa = 10.4QLLLSTGLRR214 pKa = 11.84IGEE217 pKa = 4.13ACALEE222 pKa = 4.31WGDD225 pKa = 3.94IDD227 pKa = 5.91LEE229 pKa = 4.47NGTIAINKK237 pKa = 7.38TYY239 pKa = 11.06NKK241 pKa = 9.77NLKK244 pKa = 9.76FLSTAKK250 pKa = 8.49TQSGNRR256 pKa = 11.84VISVDD261 pKa = 3.32KK262 pKa = 10.13KK263 pKa = 7.7TLRR266 pKa = 11.84SLKK269 pKa = 9.82LYY271 pKa = 9.48QMRR274 pKa = 11.84QRR276 pKa = 11.84QLFNEE281 pKa = 3.92VGARR285 pKa = 11.84VSEE288 pKa = 4.58VVFATPTRR296 pKa = 11.84KK297 pKa = 9.82YY298 pKa = 9.68FNASVRR304 pKa = 11.84QSALDD309 pKa = 3.79TRR311 pKa = 11.84CKK313 pKa = 9.91EE314 pKa = 3.83AGIEE318 pKa = 3.94RR319 pKa = 11.84FTFHH323 pKa = 7.65AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.51RR346 pKa = 11.84LGHH349 pKa = 6.41ANISMTLDD357 pKa = 3.43TYY359 pKa = 11.88GHH361 pKa = 6.98LSKK364 pKa = 11.01DD365 pKa = 3.47KK366 pKa = 10.63EE367 pKa = 4.32KK368 pKa = 11.01EE369 pKa = 3.74AVLYY373 pKa = 9.78YY374 pKa = 10.38EE375 pKa = 4.79KK376 pKa = 11.4AMNNLL381 pKa = 3.41

Molecular weight: 43.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

3408

56

562

154.9

17.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.604 ± 0.345

0.792 ± 0.254

5.663 ± 0.514

7.864 ± 0.547

4.02 ± 0.317

4.636 ± 0.47

1.79 ± 0.331

6.984 ± 0.496

9.771 ± 0.624

9.859 ± 0.605

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.054 ± 0.212

5.282 ± 0.345

3.198 ± 0.669

4.577 ± 0.233

5.047 ± 0.44

5.516 ± 0.347

6.221 ± 0.343

5.194 ± 0.34

1.027 ± 0.21

4.9 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski