Amino acid dipepetide frequency for Pseudomonas phage Epa43

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.952AlaAla: 6.952 ± 1.318
1.07AlaCys: 1.07 ± 0.318
4.011AlaAsp: 4.011 ± 0.558
9.893AlaGlu: 9.893 ± 1.798
3.743AlaPhe: 3.743 ± 0.877
7.219AlaGly: 7.219 ± 1.552
1.07AlaHis: 1.07 ± 0.452
3.476AlaIle: 3.476 ± 0.882
5.615AlaLys: 5.615 ± 1.739
9.626AlaLeu: 9.626 ± 1.099
2.406AlaMet: 2.406 ± 0.63
3.476AlaAsn: 3.476 ± 1.199
2.941AlaPro: 2.941 ± 0.271
3.209AlaGln: 3.209 ± 0.737
4.011AlaArg: 4.011 ± 1.036
5.348AlaSer: 5.348 ± 1.332
4.813AlaThr: 4.813 ± 1.894
6.15AlaVal: 6.15 ± 0.664
0.802AlaTrp: 0.802 ± 0.314
2.941AlaTyr: 2.941 ± 0.663
0.0AlaXaa: 0.0 ± 0.0
Cys
0.802CysAla: 0.802 ± 0.445
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.802CysGlu: 0.802 ± 0.294
0.267CysPhe: 0.267 ± 0.25
1.337CysGly: 1.337 ± 0.623
0.267CysHis: 0.267 ± 0.224
0.802CysIle: 0.802 ± 0.511
0.267CysLys: 0.267 ± 0.25
0.267CysLeu: 0.267 ± 0.251
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.535CysPro: 0.535 ± 0.288
0.802CysGln: 0.802 ± 0.484
0.802CysArg: 0.802 ± 0.448
1.337CysSer: 1.337 ± 0.4
0.535CysThr: 0.535 ± 0.305
0.802CysVal: 0.802 ± 0.294
0.267CysTrp: 0.267 ± 0.224
0.535CysTyr: 0.535 ± 0.318
0.0CysXaa: 0.0 ± 0.0
Asp
4.813AspAla: 4.813 ± 1.253
0.802AspCys: 0.802 ± 0.294
3.209AspAsp: 3.209 ± 0.544
8.021AspGlu: 8.021 ± 1.53
2.406AspPhe: 2.406 ± 0.473
5.615AspGly: 5.615 ± 1.242
1.337AspHis: 1.337 ± 0.419
3.743AspIle: 3.743 ± 1.103
4.011AspLys: 4.011 ± 0.979
5.08AspLeu: 5.08 ± 0.936
1.337AspMet: 1.337 ± 0.389
1.872AspAsn: 1.872 ± 0.79
3.476AspPro: 3.476 ± 0.806
2.139AspGln: 2.139 ± 0.532
2.406AspArg: 2.406 ± 0.673
3.476AspSer: 3.476 ± 0.975
1.604AspThr: 1.604 ± 0.799
3.476AspVal: 3.476 ± 0.882
2.139AspTrp: 2.139 ± 0.574
1.872AspTyr: 1.872 ± 0.648
0.0AspXaa: 0.0 ± 0.0
Glu
6.684GluAla: 6.684 ± 1.488
0.535GluCys: 0.535 ± 0.305
6.684GluAsp: 6.684 ± 1.315
5.348GluGlu: 5.348 ± 0.482
4.011GluPhe: 4.011 ± 0.867
6.15GluGly: 6.15 ± 1.199
1.604GluHis: 1.604 ± 0.549
3.743GluIle: 3.743 ± 1.4
5.08GluLys: 5.08 ± 0.959
7.487GluLeu: 7.487 ± 1.488
2.674GluMet: 2.674 ± 0.724
2.674GluAsn: 2.674 ± 0.859
3.476GluPro: 3.476 ± 0.908
1.337GluGln: 1.337 ± 0.59
4.545GluArg: 4.545 ± 1.055
3.476GluSer: 3.476 ± 0.568
2.941GluThr: 2.941 ± 0.662
4.278GluVal: 4.278 ± 1.248
1.604GluTrp: 1.604 ± 0.741
1.337GluTyr: 1.337 ± 0.665
0.0GluXaa: 0.0 ± 0.0
Phe
4.278PheAla: 4.278 ± 1.066
0.267PheCys: 0.267 ± 0.251
2.406PheAsp: 2.406 ± 0.595
3.743PheGlu: 3.743 ± 1.079
1.604PhePhe: 1.604 ± 0.804
3.743PheGly: 3.743 ± 1.202
1.07PheHis: 1.07 ± 0.443
1.337PheIle: 1.337 ± 0.638
1.872PheLys: 1.872 ± 0.575
2.941PheLeu: 2.941 ± 0.913
1.07PheMet: 1.07 ± 0.641
1.872PheAsn: 1.872 ± 0.389
1.337PhePro: 1.337 ± 0.74
0.802PheGln: 0.802 ± 0.761
3.743PheArg: 3.743 ± 1.021
2.139PheSer: 2.139 ± 0.446
2.674PheThr: 2.674 ± 1.565
2.674PheVal: 2.674 ± 0.742
1.07PheTrp: 1.07 ± 0.535
1.604PheTyr: 1.604 ± 0.72
0.0PheXaa: 0.0 ± 0.0
Gly
4.545GlyAla: 4.545 ± 1.096
0.0GlyCys: 0.0 ± 0.0
7.219GlyAsp: 7.219 ± 1.051
4.545GlyGlu: 4.545 ± 0.701
2.941GlyPhe: 2.941 ± 0.972
7.219GlyGly: 7.219 ± 0.792
1.337GlyHis: 1.337 ± 0.591
5.348GlyIle: 5.348 ± 1.482
6.15GlyLys: 6.15 ± 1.723
7.487GlyLeu: 7.487 ± 2.202
1.337GlyMet: 1.337 ± 0.558
3.476GlyAsn: 3.476 ± 0.803
3.476GlyPro: 3.476 ± 1.037
4.278GlyGln: 4.278 ± 1.381
4.813GlyArg: 4.813 ± 0.446
6.15GlySer: 6.15 ± 1.16
4.813GlyThr: 4.813 ± 2.441
7.487GlyVal: 7.487 ± 0.948
2.406GlyTrp: 2.406 ± 0.804
1.604GlyTyr: 1.604 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
2.406HisAla: 2.406 ± 0.492
0.267HisCys: 0.267 ± 0.221
1.07HisAsp: 1.07 ± 0.609
1.872HisGlu: 1.872 ± 0.677
1.07HisPhe: 1.07 ± 0.413
1.604HisGly: 1.604 ± 0.611
0.0HisHis: 0.0 ± 0.0
1.604HisIle: 1.604 ± 0.7
1.872HisLys: 1.872 ± 0.803
2.406HisLeu: 2.406 ± 1.146
0.535HisMet: 0.535 ± 0.318
0.535HisAsn: 0.535 ± 0.288
0.802HisPro: 0.802 ± 0.429
0.267HisGln: 0.267 ± 0.236
1.337HisArg: 1.337 ± 0.754
1.337HisSer: 1.337 ± 0.637
1.07HisThr: 1.07 ± 0.403
0.802HisVal: 0.802 ± 0.309
0.267HisTrp: 0.267 ± 0.251
0.802HisTyr: 0.802 ± 0.314
0.0HisXaa: 0.0 ± 0.0
Ile
5.08IleAla: 5.08 ± 1.569
0.267IleCys: 0.267 ± 0.224
3.476IleAsp: 3.476 ± 0.895
3.476IleGlu: 3.476 ± 0.726
2.406IlePhe: 2.406 ± 0.731
3.476IleGly: 3.476 ± 0.703
1.604IleHis: 1.604 ± 0.523
2.139IleIle: 2.139 ± 0.629
2.674IleLys: 2.674 ± 0.988
4.011IleLeu: 4.011 ± 1.097
1.07IleMet: 1.07 ± 0.478
1.604IleAsn: 1.604 ± 0.652
2.674IlePro: 2.674 ± 0.777
3.209IleGln: 3.209 ± 0.516
3.743IleArg: 3.743 ± 1.64
3.209IleSer: 3.209 ± 0.74
2.674IleThr: 2.674 ± 1.438
1.872IleVal: 1.872 ± 0.563
0.535IleTrp: 0.535 ± 0.318
1.604IleTyr: 1.604 ± 0.648
0.0IleXaa: 0.0 ± 0.0
Lys
8.021LysAla: 8.021 ± 2.12
0.267LysCys: 0.267 ± 0.224
2.941LysAsp: 2.941 ± 0.866
4.011LysGlu: 4.011 ± 0.824
2.139LysPhe: 2.139 ± 0.753
4.278LysGly: 4.278 ± 1.123
1.604LysHis: 1.604 ± 0.836
1.872LysIle: 1.872 ± 0.54
2.406LysLys: 2.406 ± 1.219
4.545LysLeu: 4.545 ± 0.84
1.872LysMet: 1.872 ± 0.844
2.406LysAsn: 2.406 ± 0.917
1.07LysPro: 1.07 ± 0.551
0.535LysGln: 0.535 ± 0.28
3.209LysArg: 3.209 ± 0.629
2.406LysSer: 2.406 ± 0.803
3.476LysThr: 3.476 ± 1.266
4.278LysVal: 4.278 ± 1.027
2.139LysTrp: 2.139 ± 0.72
3.209LysTyr: 3.209 ± 0.629
0.0LysXaa: 0.0 ± 0.0
Leu
8.824LeuAla: 8.824 ± 2.026
1.872LeuCys: 1.872 ± 0.745
5.882LeuAsp: 5.882 ± 1.168
4.011LeuGlu: 4.011 ± 0.806
2.406LeuPhe: 2.406 ± 0.919
6.417LeuGly: 6.417 ± 1.193
2.139LeuHis: 2.139 ± 0.791
3.209LeuIle: 3.209 ± 0.612
3.476LeuLys: 3.476 ± 0.807
5.882LeuLeu: 5.882 ± 1.198
3.743LeuMet: 3.743 ± 0.575
3.476LeuAsn: 3.476 ± 1.018
5.615LeuPro: 5.615 ± 1.693
4.813LeuGln: 4.813 ± 1.234
5.615LeuArg: 5.615 ± 1.066
5.348LeuSer: 5.348 ± 1.369
4.545LeuThr: 4.545 ± 1.535
4.545LeuVal: 4.545 ± 0.518
1.07LeuTrp: 1.07 ± 0.438
1.337LeuTyr: 1.337 ± 0.591
0.0LeuXaa: 0.0 ± 0.0
Met
3.209MetAla: 3.209 ± 0.585
0.535MetCys: 0.535 ± 0.288
1.604MetAsp: 1.604 ± 0.266
0.802MetGlu: 0.802 ± 0.324
1.337MetPhe: 1.337 ± 0.527
2.139MetGly: 2.139 ± 1.062
0.267MetHis: 0.267 ± 0.289
1.604MetIle: 1.604 ± 0.926
1.337MetLys: 1.337 ± 0.278
2.139MetLeu: 2.139 ± 0.395
1.07MetMet: 1.07 ± 0.475
0.535MetAsn: 0.535 ± 0.288
0.802MetPro: 0.802 ± 0.476
1.872MetGln: 1.872 ± 1.056
1.337MetArg: 1.337 ± 0.496
1.872MetSer: 1.872 ± 0.691
1.07MetThr: 1.07 ± 0.438
1.604MetVal: 1.604 ± 0.593
0.267MetTrp: 0.267 ± 0.236
0.267MetTyr: 0.267 ± 0.251
0.0MetXaa: 0.0 ± 0.0
Asn
2.139AsnAla: 2.139 ± 0.594
0.535AsnCys: 0.535 ± 0.288
1.337AsnAsp: 1.337 ± 0.615
2.674AsnGlu: 2.674 ± 0.599
1.604AsnPhe: 1.604 ± 0.518
5.348AsnGly: 5.348 ± 1.248
0.535AsnHis: 0.535 ± 0.32
1.872AsnIle: 1.872 ± 0.605
1.337AsnLys: 1.337 ± 0.8
3.743AsnLeu: 3.743 ± 0.959
0.535AsnMet: 0.535 ± 0.401
0.0AsnAsn: 0.0 ± 0.0
1.337AsnPro: 1.337 ± 0.622
0.535AsnGln: 0.535 ± 0.346
2.941AsnArg: 2.941 ± 0.499
0.802AsnSer: 0.802 ± 0.309
1.07AsnThr: 1.07 ± 0.625
4.011AsnVal: 4.011 ± 1.214
0.802AsnTrp: 0.802 ± 0.519
1.07AsnTyr: 1.07 ± 0.329
0.0AsnXaa: 0.0 ± 0.0
Pro
5.882ProAla: 5.882 ± 1.022
0.0ProCys: 0.0 ± 0.0
4.011ProAsp: 4.011 ± 1.617
5.08ProGlu: 5.08 ± 0.862
2.406ProPhe: 2.406 ± 0.628
4.813ProGly: 4.813 ± 1.537
0.802ProHis: 0.802 ± 0.349
1.604ProIle: 1.604 ± 0.65
2.406ProLys: 2.406 ± 0.861
2.139ProLeu: 2.139 ± 1.007
0.802ProMet: 0.802 ± 0.479
1.604ProAsn: 1.604 ± 0.638
1.337ProPro: 1.337 ± 0.403
0.802ProGln: 0.802 ± 0.457
2.941ProArg: 2.941 ± 1.007
1.872ProSer: 1.872 ± 0.792
1.872ProThr: 1.872 ± 0.492
2.139ProVal: 2.139 ± 0.573
0.802ProTrp: 0.802 ± 0.466
1.604ProTyr: 1.604 ± 0.392
0.0ProXaa: 0.0 ± 0.0
Gln
3.209GlnAla: 3.209 ± 0.598
0.267GlnCys: 0.267 ± 0.25
1.604GlnAsp: 1.604 ± 0.604
4.278GlnGlu: 4.278 ± 0.979
1.604GlnPhe: 1.604 ± 0.498
3.209GlnGly: 3.209 ± 0.608
1.07GlnHis: 1.07 ± 0.287
0.802GlnIle: 0.802 ± 0.374
2.674GlnLys: 2.674 ± 0.986
4.545GlnLeu: 4.545 ± 1.548
1.07GlnMet: 1.07 ± 0.425
0.267GlnAsn: 0.267 ± 0.348
1.07GlnPro: 1.07 ± 0.575
4.011GlnGln: 4.011 ± 0.925
2.941GlnArg: 2.941 ± 0.836
1.337GlnSer: 1.337 ± 0.264
2.139GlnThr: 2.139 ± 0.542
2.941GlnVal: 2.941 ± 1.006
0.535GlnTrp: 0.535 ± 0.305
1.604GlnTyr: 1.604 ± 0.507
0.0GlnXaa: 0.0 ± 0.0
Arg
4.278ArgAla: 4.278 ± 1.302
0.535ArgCys: 0.535 ± 0.473
3.476ArgAsp: 3.476 ± 1.312
2.406ArgGlu: 2.406 ± 0.63
1.604ArgPhe: 1.604 ± 0.618
6.15ArgGly: 6.15 ± 1.321
3.743ArgHis: 3.743 ± 1.073
4.545ArgIle: 4.545 ± 0.803
4.011ArgLys: 4.011 ± 1.005
4.011ArgLeu: 4.011 ± 0.898
2.674ArgMet: 2.674 ± 0.802
1.872ArgAsn: 1.872 ± 0.667
2.674ArgPro: 2.674 ± 0.753
2.941ArgGln: 2.941 ± 0.627
5.08ArgArg: 5.08 ± 1.333
3.209ArgSer: 3.209 ± 0.981
2.674ArgThr: 2.674 ± 0.933
3.476ArgVal: 3.476 ± 0.905
0.267ArgTrp: 0.267 ± 0.236
1.872ArgTyr: 1.872 ± 0.654
0.0ArgXaa: 0.0 ± 0.0
Ser
3.743SerAla: 3.743 ± 1.66
1.604SerCys: 1.604 ± 0.425
2.139SerAsp: 2.139 ± 0.597
3.743SerGlu: 3.743 ± 1.126
3.209SerPhe: 3.209 ± 1.107
4.813SerGly: 4.813 ± 0.337
0.535SerHis: 0.535 ± 0.501
3.476SerIle: 3.476 ± 0.865
3.209SerLys: 3.209 ± 0.803
3.476SerLeu: 3.476 ± 0.506
1.337SerMet: 1.337 ± 0.758
1.872SerAsn: 1.872 ± 0.624
3.476SerPro: 3.476 ± 0.713
2.406SerGln: 2.406 ± 0.942
2.941SerArg: 2.941 ± 0.709
1.872SerSer: 1.872 ± 0.569
1.604SerThr: 1.604 ± 0.731
3.209SerVal: 3.209 ± 0.466
1.604SerTrp: 1.604 ± 0.4
2.139SerTyr: 2.139 ± 0.416
0.0SerXaa: 0.0 ± 0.0
Thr
5.08ThrAla: 5.08 ± 1.19
0.802ThrCys: 0.802 ± 0.324
3.209ThrAsp: 3.209 ± 0.968
2.139ThrGlu: 2.139 ± 0.571
2.139ThrPhe: 2.139 ± 0.825
4.278ThrGly: 4.278 ± 1.495
1.07ThrHis: 1.07 ± 0.521
3.209ThrIle: 3.209 ± 1.027
2.674ThrLys: 2.674 ± 0.71
4.278ThrLeu: 4.278 ± 1.679
0.535ThrMet: 0.535 ± 0.437
1.872ThrAsn: 1.872 ± 0.891
2.674ThrPro: 2.674 ± 0.956
1.604ThrGln: 1.604 ± 0.718
2.139ThrArg: 2.139 ± 0.677
1.604ThrSer: 1.604 ± 0.69
2.941ThrThr: 2.941 ± 0.935
2.941ThrVal: 2.941 ± 0.462
0.802ThrTrp: 0.802 ± 0.414
1.872ThrTyr: 1.872 ± 0.559
0.0ThrXaa: 0.0 ± 0.0
Val
4.813ValAla: 4.813 ± 0.967
0.267ValCys: 0.267 ± 0.224
5.882ValAsp: 5.882 ± 0.782
5.08ValGlu: 5.08 ± 1.159
2.941ValPhe: 2.941 ± 0.65
3.476ValGly: 3.476 ± 0.685
1.07ValHis: 1.07 ± 0.666
3.743ValIle: 3.743 ± 1.109
3.209ValLys: 3.209 ± 0.864
4.813ValLeu: 4.813 ± 0.896
0.802ValMet: 0.802 ± 0.327
2.139ValAsn: 2.139 ± 0.613
4.011ValPro: 4.011 ± 0.578
4.011ValGln: 4.011 ± 0.814
3.743ValArg: 3.743 ± 1.257
2.674ValSer: 2.674 ± 0.606
3.476ValThr: 3.476 ± 1.397
4.545ValVal: 4.545 ± 1.189
0.535ValTrp: 0.535 ± 0.318
1.604ValTyr: 1.604 ± 0.618
0.0ValXaa: 0.0 ± 0.0
Trp
2.139TrpAla: 2.139 ± 0.719
0.267TrpCys: 0.267 ± 0.251
1.604TrpAsp: 1.604 ± 0.502
1.604TrpGlu: 1.604 ± 0.697
0.802TrpPhe: 0.802 ± 0.349
1.604TrpGly: 1.604 ± 0.557
0.267TrpHis: 0.267 ± 0.221
0.802TrpIle: 0.802 ± 0.438
1.337TrpLys: 1.337 ± 0.498
1.872TrpLeu: 1.872 ± 0.611
0.0TrpMet: 0.0 ± 0.0
0.802TrpAsn: 0.802 ± 0.438
0.267TrpPro: 0.267 ± 0.25
0.535TrpGln: 0.535 ± 0.28
1.07TrpArg: 1.07 ± 0.635
0.535TrpSer: 0.535 ± 0.305
0.802TrpThr: 0.802 ± 0.324
0.802TrpVal: 0.802 ± 0.466
0.802TrpTrp: 0.802 ± 0.466
1.07TrpTyr: 1.07 ± 0.329
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.139TyrAla: 2.139 ± 0.596
0.267TyrCys: 0.267 ± 0.289
1.07TyrAsp: 1.07 ± 0.475
2.139TyrGlu: 2.139 ± 0.837
1.604TyrPhe: 1.604 ± 0.548
3.743TyrGly: 3.743 ± 0.964
0.535TyrHis: 0.535 ± 0.415
1.872TyrIle: 1.872 ± 0.879
1.07TyrLys: 1.07 ± 0.55
3.476TyrLeu: 3.476 ± 0.934
0.535TyrMet: 0.535 ± 0.264
1.872TyrAsn: 1.872 ± 0.546
1.872TyrPro: 1.872 ± 0.842
1.07TyrGln: 1.07 ± 0.505
2.139TyrArg: 2.139 ± 0.597
2.406TyrSer: 2.406 ± 0.907
1.07TyrThr: 1.07 ± 0.473
0.802TyrVal: 0.802 ± 0.374
0.267TyrTrp: 0.267 ± 0.221
0.267TyrTyr: 0.267 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8 proteins (3741 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski