Cupriavidus sp. HPC(L)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; unclassified Cupriavidus

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V2IL98|V2IL98_9BURK Succinyldiaminopimelate aminotransferase OS=Cupriavidus sp. HPC(L) OX=1217418 GN=B551_0212410 PE=4 SV=1
MM1 pKa = 6.46QQEE4 pKa = 3.85AVAYY8 pKa = 6.5KK9 pKa = 9.45TWVCLICGWVYY20 pKa = 11.08DD21 pKa = 4.83EE22 pKa = 4.29EE23 pKa = 4.75QGWPDD28 pKa = 4.15DD29 pKa = 4.55GIAPGTRR36 pKa = 11.84WEE38 pKa = 4.98EE39 pKa = 3.77IPEE42 pKa = 3.87DD43 pKa = 3.89WRR45 pKa = 11.84CPEE48 pKa = 4.36CDD50 pKa = 3.18VAKK53 pKa = 10.77ADD55 pKa = 3.73FAMIEE60 pKa = 3.97II61 pKa = 4.71

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V2G331|V2G331_9BURK Fatty acid hydroxylase OS=Cupriavidus sp. HPC(L) OX=1217418 GN=B551_0224525 PE=4 SV=1
MM1 pKa = 7.0FARR4 pKa = 11.84RR5 pKa = 11.84FVSFAQARR13 pKa = 11.84RR14 pKa = 11.84LARR17 pKa = 11.84LAGVRR22 pKa = 11.84VARR25 pKa = 11.84QIANAARR32 pKa = 11.84MRR34 pKa = 11.84APLMRR39 pKa = 11.84APRR42 pKa = 11.84MAAGLAAVPSRR53 pKa = 11.84RR54 pKa = 11.84ITRR57 pKa = 11.84RR58 pKa = 11.84IPAPLDD64 pKa = 3.28PARR67 pKa = 11.84FPAA70 pKa = 4.68

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4779

0

4779

1542997

29

3960

322.9

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.309 ± 0.052

0.911 ± 0.012

5.441 ± 0.025

5.18 ± 0.033

3.353 ± 0.025

8.609 ± 0.045

2.225 ± 0.018

4.428 ± 0.025

2.735 ± 0.029

10.522 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.016

2.53 ± 0.028

5.468 ± 0.029

3.792 ± 0.026

7.535 ± 0.04

5.029 ± 0.031

5.149 ± 0.034

7.606 ± 0.029

1.388 ± 0.015

2.32 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski