Bos taurus papillomavirus 18
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2K2B2|A0A1B2K2B2_9PAPI Minor capsid protein L2 OS=Bos taurus papillomavirus 18 OX=1887216 GN=L2 PE=3 SV=1
MM1 pKa = 7.33 ATNKK5 pKa = 9.88 RR6 pKa = 11.84 SRR8 pKa = 11.84 VKK10 pKa = 10.24 RR11 pKa = 11.84 ASVSDD16 pKa = 3.82 LAKK19 pKa = 9.71 TCATGDD25 pKa = 3.91 CPEE28 pKa = 4.23 DD29 pKa = 3.57 VYY31 pKa = 12.03 NKK33 pKa = 8.78 VTGNTLADD41 pKa = 4.14 KK42 pKa = 10.41 ILRR45 pKa = 11.84 FLSPFLYY52 pKa = 10.55 FGGLSIGTGAGGGGRR67 pKa = 11.84 FGYY70 pKa = 10.22 RR71 pKa = 11.84 PIGGTSGGGRR81 pKa = 11.84 PTVTVRR87 pKa = 11.84 PVVPVEE93 pKa = 4.29 TIAPEE98 pKa = 3.93 IEE100 pKa = 4.15 VGGTIDD106 pKa = 3.28 SSGVIDD112 pKa = 3.66 AAGPSVIEE120 pKa = 3.85 LTPINGDD127 pKa = 3.28 PTVIDD132 pKa = 3.91 VAPDD136 pKa = 3.27 TAINEE141 pKa = 4.27 TGPPILEE148 pKa = 4.04 VTPEE152 pKa = 3.74 VRR154 pKa = 11.84 VTVSGRR160 pKa = 11.84 EE161 pKa = 4.07 TEE163 pKa = 4.41 TVVQNPTFTPDD174 pKa = 3.07 PSTINTSHH182 pKa = 6.7 NEE184 pKa = 4.09 NIVFEE189 pKa = 4.82 PNTNAVYY196 pKa = 10.53 VGSTAEE202 pKa = 3.83 TSEE205 pKa = 4.36 EE206 pKa = 3.86 IEE208 pKa = 3.97 LSVFSRR214 pKa = 11.84 GDD216 pKa = 3.31 TFSDD220 pKa = 3.49 SFATEE225 pKa = 3.96 VEE227 pKa = 4.38 EE228 pKa = 4.34 TSFDD232 pKa = 3.29 ASTPLVRR239 pKa = 11.84 QAEE242 pKa = 4.21 GRR244 pKa = 11.84 PINRR248 pKa = 11.84 FGRR251 pKa = 11.84 YY252 pKa = 6.77 FQQVPVSEE260 pKa = 4.82 PEE262 pKa = 4.06 FLSRR266 pKa = 11.84 PASMVTFGNAAYY278 pKa = 10.33 DD279 pKa = 3.88 PDD281 pKa = 3.46 ATAIFEE287 pKa = 4.8 DD288 pKa = 4.48 DD289 pKa = 4.45 LRR291 pKa = 11.84 DD292 pKa = 4.03 LEE294 pKa = 5.57 DD295 pKa = 3.55 IAAAPYY301 pKa = 10.54 TDD303 pKa = 5.01 FSDD306 pKa = 4.37 LKK308 pKa = 9.09 TLSRR312 pKa = 11.84 PYY314 pKa = 9.78 YY315 pKa = 9.92 SRR317 pKa = 11.84 GGRR320 pKa = 11.84 TGRR323 pKa = 11.84 LIVSRR328 pKa = 11.84 VGTKK332 pKa = 8.23 STIHH336 pKa = 5.6 TRR338 pKa = 11.84 SGLSIGASVHH348 pKa = 6.23 YY349 pKa = 8.72 YY350 pKa = 10.24 TEE352 pKa = 4.49 LSTINEE358 pKa = 4.21 PTVAEE363 pKa = 4.04 QPPFEE368 pKa = 5.46 ISDD371 pKa = 3.95 FVGSTSADD379 pKa = 3.16 EE380 pKa = 5.07 AFINSQGTEE389 pKa = 3.95 EE390 pKa = 4.76 FEE392 pKa = 5.07 LISLRR397 pKa = 11.84 DD398 pKa = 3.71 PSVEE402 pKa = 4.09 TLDD405 pKa = 3.55 EE406 pKa = 4.06 TSEE409 pKa = 4.11 YY410 pKa = 11.03 SEE412 pKa = 4.32 TALLDD417 pKa = 4.25 HH418 pKa = 7.22 IEE420 pKa = 4.52 EE421 pKa = 4.19 IGEE424 pKa = 3.99 NLRR427 pKa = 11.84 LIIQSDD433 pKa = 3.54 EE434 pKa = 4.23 RR435 pKa = 11.84 EE436 pKa = 3.84 QTIISVPSFNEE447 pKa = 3.98 SVAEE451 pKa = 3.93 NAVSIVVDD459 pKa = 3.81 YY460 pKa = 10.77 PNSTSEE466 pKa = 4.38 SPATNYY472 pKa = 9.75 PMEE475 pKa = 4.63 YY476 pKa = 8.14 PTDD479 pKa = 4.16 TISPSQPATPGLTPLIPSSLQPTNYY504 pKa = 9.5 FDD506 pKa = 3.22 YY507 pKa = 9.85 WQLFEE512 pKa = 4.52 PSLWRR517 pKa = 11.84 SRR519 pKa = 11.84 KK520 pKa = 8.93 RR521 pKa = 11.84 KK522 pKa = 9.47 RR523 pKa = 11.84 NVYY526 pKa = 9.2 YY527 pKa = 10.94
Molecular weight: 57.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.432
IPC2_protein 4.368
IPC_protein 4.329
Toseland 4.164
ProMoST 4.418
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.126
Rodwell 4.151
Grimsley 4.062
Solomon 4.266
Lehninger 4.215
Nozaki 4.368
DTASelect 4.52
Thurlkill 4.164
EMBOSS 4.139
Sillero 4.431
Patrickios 3.719
IPC_peptide 4.266
IPC2_peptide 4.418
IPC2.peptide.svr19 4.37
Protein with the highest isoelectric point:
>tr|A0A1B2K215|A0A1B2K215_9PAPI Protein E6 OS=Bos taurus papillomavirus 18 OX=1887216 GN=E6 PE=3 SV=1
MM1 pKa = 7.07 EE2 pKa = 4.86 TLLSRR7 pKa = 11.84 LDD9 pKa = 3.54 AVRR12 pKa = 11.84 EE13 pKa = 3.97 EE14 pKa = 4.81 LLTLYY19 pKa = 10.65 EE20 pKa = 4.41 SGSNRR25 pKa = 11.84 LEE27 pKa = 3.82 DD28 pKa = 5.03 QIRR31 pKa = 11.84 HH32 pKa = 4.56 WKK34 pKa = 9.73 LLRR37 pKa = 11.84 KK38 pKa = 9.34 EE39 pKa = 3.87 YY40 pKa = 11.36 VMLHH44 pKa = 6.37 FARR47 pKa = 11.84 KK48 pKa = 8.93 HH49 pKa = 5.38 DD50 pKa = 3.68 IYY52 pKa = 11.11 RR53 pKa = 11.84 LGLSSVPTLQVSQAKK68 pKa = 9.69 AKK70 pKa = 9.82 EE71 pKa = 4.19 AIRR74 pKa = 11.84 MEE76 pKa = 4.35 LLLEE80 pKa = 4.17 SLAKK84 pKa = 9.99 SEE86 pKa = 4.36 YY87 pKa = 10.58 SSEE90 pKa = 3.82 PWTLSEE96 pKa = 4.3 TSIEE100 pKa = 4.1 MLEE103 pKa = 4.44 TPPKK107 pKa = 10.8 DD108 pKa = 3.29 CFKK111 pKa = 10.77 KK112 pKa = 10.07 HH113 pKa = 4.81 GVTVEE118 pKa = 3.83 VLFDD122 pKa = 4.11 GDD124 pKa = 4.06 PEE126 pKa = 4.36 NVMTYY131 pKa = 8.78 TNWKK135 pKa = 9.42 DD136 pKa = 2.76 IYY138 pKa = 10.28 YY139 pKa = 8.17 QTEE142 pKa = 4.27 SGWEE146 pKa = 3.91 KK147 pKa = 8.31 TTGHH151 pKa = 5.74 VNYY154 pKa = 9.89 DD155 pKa = 3.06 GCFYY159 pKa = 11.23 YY160 pKa = 10.36 EE161 pKa = 4.55 GRR163 pKa = 11.84 SQVYY167 pKa = 7.43 FVRR170 pKa = 11.84 FAPDD174 pKa = 3.0 AARR177 pKa = 11.84 YY178 pKa = 9.14 GSTGEE183 pKa = 3.89 WEE185 pKa = 3.88 VRR187 pKa = 11.84 YY188 pKa = 10.33 QNDD191 pKa = 2.82 VFFPVASVSSTSEE204 pKa = 3.43 WCPPSTGSGIQPWGDD219 pKa = 3.52 KK220 pKa = 11.09 LPVDD224 pKa = 4.09 TCLGSAGRR232 pKa = 11.84 PAQTARR238 pKa = 11.84 RR239 pKa = 11.84 SPDD242 pKa = 2.99 SSGSSATSTTTSRR255 pKa = 11.84 RR256 pKa = 11.84 RR257 pKa = 11.84 RR258 pKa = 11.84 KK259 pKa = 9.29 SVRR262 pKa = 11.84 IRR264 pKa = 11.84 KK265 pKa = 7.86 ATAPQEE271 pKa = 4.31 AQRR274 pKa = 11.84 SSPRR278 pKa = 11.84 SSSGAEE284 pKa = 4.12 DD285 pKa = 3.3 GRR287 pKa = 11.84 QARR290 pKa = 11.84 FQPGGRR296 pKa = 11.84 IPPGSHH302 pKa = 4.05 QQVEE306 pKa = 4.32 RR307 pKa = 11.84 GARR310 pKa = 11.84 EE311 pKa = 3.59 NPRR314 pKa = 11.84 RR315 pKa = 11.84 RR316 pKa = 11.84 RR317 pKa = 11.84 SRR319 pKa = 11.84 LIQLLADD326 pKa = 3.96 AADD329 pKa = 3.87 PPIAVLRR336 pKa = 11.84 GASNSLKK343 pKa = 10.15 CLRR346 pKa = 11.84 YY347 pKa = 9.45 RR348 pKa = 11.84 LNRR351 pKa = 11.84 RR352 pKa = 11.84 HH353 pKa = 6.5 RR354 pKa = 11.84 GLFNHH359 pKa = 7.45 ISTTWSWADD368 pKa = 3.24 EE369 pKa = 4.36 RR370 pKa = 11.84 GPGANARR377 pKa = 11.84 MIIAFTNSTQRR388 pKa = 11.84 QTFLDD393 pKa = 4.3 TVSLPRR399 pKa = 11.84 PITYY403 pKa = 10.31 FLGNFSSLL411 pKa = 3.44
Molecular weight: 46.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.911
IPC2_protein 8.96
IPC_protein 9.033
Toseland 9.414
ProMoST 9.341
Dawson 9.75
Bjellqvist 9.56
Wikipedia 9.984
Rodwell 9.867
Grimsley 9.853
Solomon 9.794
Lehninger 9.736
Nozaki 9.575
DTASelect 9.516
Thurlkill 9.575
EMBOSS 9.867
Sillero 9.706
Patrickios 4.94
IPC_peptide 9.78
IPC2_peptide 8.419
IPC2.peptide.svr19 7.866
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2293
88
608
382.2
43.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.582 ± 0.664
2.093 ± 0.816
5.713 ± 0.368
7.021 ± 0.678
4.405 ± 0.274
5.931 ± 0.73
2.268 ± 0.496
5.233 ± 0.592
4.666 ± 0.936
8.112 ± 0.762
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.919 ± 0.458
5.015 ± 1.012
5.451 ± 0.782
4.187 ± 0.638
6.106 ± 0.996
8.33 ± 0.848
6.847 ± 1.056
5.975 ± 0.595
1.526 ± 0.347
3.62 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here