Elephant endotheliotropic herpesvirus 5
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075CZJ7|A0A075CZJ7_9BETA Membrane protein EE28 OS=Elephant endotheliotropic herpesvirus 5 OX=768738 GN=EE28 PE=4 SV=1
MM1 pKa = 7.7 NIRR4 pKa = 11.84 YY5 pKa = 9.22 SADD8 pKa = 3.19 CTKK11 pKa = 10.44 ILEE14 pKa = 4.63 DD15 pKa = 3.94 LDD17 pKa = 4.82 NYY19 pKa = 11.32 LEE21 pKa = 4.54 TPLLYY26 pKa = 10.81 NNRR29 pKa = 11.84 DD30 pKa = 3.82 DD31 pKa = 5.16 GNSSVDD37 pKa = 3.49 TPPPLEE43 pKa = 4.02 TSEE46 pKa = 5.78 DD47 pKa = 3.57 GDD49 pKa = 4.03 EE50 pKa = 4.32 NFVVLTDD57 pKa = 5.61 LITTSASTSCPEE69 pKa = 4.99 PEE71 pKa = 4.4 DD72 pKa = 3.57 QDD74 pKa = 3.66 TEE76 pKa = 4.64 RR77 pKa = 11.84 IYY79 pKa = 11.07 LPRR82 pKa = 11.84 DD83 pKa = 3.47 FSSLSEE89 pKa = 5.47 DD90 pKa = 3.33 IPCYY94 pKa = 7.27 EE95 pKa = 4.33 TNVEE99 pKa = 4.02 LSQRR103 pKa = 11.84 KK104 pKa = 6.78 WVGTNDD110 pKa = 3.5 TTPSDD115 pKa = 4.28 EE116 pKa = 4.55 NPVSAGQGSQRR127 pKa = 11.84 KK128 pKa = 8.92 YY129 pKa = 10.27 IYY131 pKa = 10.36 PGDD134 pKa = 3.98 LEE136 pKa = 4.46 NVSTLFNSVFFFNRR150 pKa = 3.09
Molecular weight: 16.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.928
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.605
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.935
Patrickios 2.74
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A075CYD2|A0A075CYD2_9BETA Membrane protein EE42 OS=Elephant endotheliotropic herpesvirus 5 OX=768738 GN=EE42 PE=4 SV=1
MM1 pKa = 7.48 SFYY4 pKa = 10.84 FFLIWPRR11 pKa = 11.84 STIKK15 pKa = 10.51 LSYY18 pKa = 9.87 IRR20 pKa = 11.84 ARR22 pKa = 11.84 HH23 pKa = 5.62 LRR25 pKa = 11.84 NKK27 pKa = 9.59 LRR29 pKa = 11.84 KK30 pKa = 8.34 QLFYY34 pKa = 11.05 SLCLSILNGLCYY46 pKa = 10.3 ALHH49 pKa = 6.64 ALVIYY54 pKa = 9.88 HH55 pKa = 7.02 LKK57 pKa = 8.8 QNYY60 pKa = 7.14 MPWHH64 pKa = 5.24 VHH66 pKa = 4.57 RR67 pKa = 11.84 QYY69 pKa = 11.36 LYY71 pKa = 8.09 TWVWKK76 pKa = 9.88 HH77 pKa = 5.51
Molecular weight: 9.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 10.233
IPC_protein 10.599
Toseland 10.16
ProMoST 10.028
Dawson 10.452
Bjellqvist 10.204
Wikipedia 10.687
Rodwell 10.701
Grimsley 10.555
Solomon 10.482
Lehninger 10.423
Nozaki 10.189
DTASelect 10.204
Thurlkill 10.262
EMBOSS 10.584
Sillero 10.365
Patrickios 10.16
IPC_peptide 10.467
IPC2_peptide 9.282
IPC2.peptide.svr19 8.166
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
115
0
115
48243
67
2276
419.5
47.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.749 ± 0.134
2.608 ± 0.147
5.317 ± 0.185
4.954 ± 0.14
4.931 ± 0.19
4.562 ± 0.184
2.651 ± 0.108
6.523 ± 0.221
5.047 ± 0.149
9.697 ± 0.249
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.566 ± 0.116
5.584 ± 0.159
4.542 ± 0.209
3.254 ± 0.197
5.029 ± 0.178
8.538 ± 0.341
7.406 ± 0.312
6.855 ± 0.132
0.939 ± 0.069
4.247 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here