marine bacterium AO1-C

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; unclassified Cytophagaceae

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8907 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q3GT67|A0A1Q3GT67_9BACT Methionyl-tRNA formyltransferase OS=marine bacterium AO1-C OX=1905359 GN=BKI52_21045 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.41FKK4 pKa = 10.28IYY6 pKa = 10.81NYY8 pKa = 9.26ILCLVASVGMMACEE22 pKa = 4.0DD23 pKa = 4.24VIDD26 pKa = 4.0VTLGDD31 pKa = 4.16STPQLTVDD39 pKa = 3.47AWISNQLTTQTFRR52 pKa = 11.84LSISQDD58 pKa = 3.28YY59 pKa = 10.57FNNNPTAGASGATVVVTDD77 pKa = 4.41NLGNTYY83 pKa = 10.88NFAEE87 pKa = 4.68STANPGNYY95 pKa = 8.58TSNFQGAVDD104 pKa = 3.43VTYY107 pKa = 9.97TLNVQYY113 pKa = 10.4QGQEE117 pKa = 3.99YY118 pKa = 9.85QATTTLSRR126 pKa = 11.84VPPIDD131 pKa = 3.31SLVFTKK137 pKa = 10.77AEE139 pKa = 4.07EE140 pKa = 4.2ADD142 pKa = 3.76GGPGSVSTGYY152 pKa = 9.82QAEE155 pKa = 4.54FFATDD160 pKa = 3.12IPGVGDD166 pKa = 3.82FYY168 pKa = 11.09RR169 pKa = 11.84IKK171 pKa = 9.75TYY173 pKa = 11.05QNGVLMNKK181 pKa = 9.95PSDD184 pKa = 3.51LTSFQDD190 pKa = 3.82LNVDD194 pKa = 4.04GLPFILPVRR203 pKa = 11.84LSINPTNEE211 pKa = 3.83DD212 pKa = 2.89NDD214 pKa = 3.94GFKK217 pKa = 10.57QGDD220 pKa = 3.82MVKK223 pKa = 10.37VEE225 pKa = 4.44LLSISEE231 pKa = 4.06EE232 pKa = 3.78AFLFFDD238 pKa = 4.43QLEE241 pKa = 4.5TQTNNGGLFADD252 pKa = 6.06PIANVPTNISNVNPSGPKK270 pKa = 9.93AVGFFYY276 pKa = 10.9ASGVSTIEE284 pKa = 5.16GIAQQ288 pKa = 3.32

Molecular weight: 31.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q3H3S3|A0A1Q3H3S3_9BACT Uncharacterized protein OS=marine bacterium AO1-C OX=1905359 GN=BKI52_04125 PE=4 SV=1
MM1 pKa = 7.75GKK3 pKa = 9.74RR4 pKa = 11.84LLRR7 pKa = 11.84RR8 pKa = 11.84LGSKK12 pKa = 10.26SFIFCDD18 pKa = 4.94PIAHH22 pKa = 5.91EE23 pKa = 4.58TSQKK27 pKa = 10.23EE28 pKa = 3.86YY29 pKa = 10.83FRR31 pKa = 11.84AGRR34 pKa = 11.84CALFWLLLLGSAATILILRR53 pKa = 11.84SRR55 pKa = 11.84TTGAGMSLIFPILITGLGAIVMFGLTFYY83 pKa = 10.68HH84 pKa = 6.15FVVGMFLL91 pKa = 3.51

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8907

0

8907

3210568

42

4790

360.5

40.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.792 ± 0.024

0.711 ± 0.008

4.972 ± 0.019

5.896 ± 0.033

4.919 ± 0.02

6.286 ± 0.034

1.981 ± 0.012

6.896 ± 0.019

7.684 ± 0.041

9.871 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.013

5.683 ± 0.027

3.628 ± 0.017

5.008 ± 0.026

4.07 ± 0.016

6.051 ± 0.023

5.94 ± 0.041

6.102 ± 0.021

1.244 ± 0.009

4.136 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski