Lentibacillus persicus
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3300 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I1S6A7|A0A1I1S6A7_9BACI Endonuclease I OS=Lentibacillus persicus OX=640948 GN=SAMN05216238_101253 PE=4 SV=1
MM1 pKa = 7.27 KK2 pKa = 10.29 KK3 pKa = 10.59 KK4 pKa = 10.05 MIIILSSIVVLLVALYY20 pKa = 9.25 FVVDD24 pKa = 3.93 YY25 pKa = 11.21 KK26 pKa = 11.12 NQQAMGDD33 pKa = 3.7 NEE35 pKa = 4.1 NPYY38 pKa = 10.78 GKK40 pKa = 10.13 EE41 pKa = 4.16 DD42 pKa = 4.22 LDD44 pKa = 4.24 QATIDD49 pKa = 3.79 QLDD52 pKa = 3.56 NPNYY56 pKa = 9.78 QNQILPDD63 pKa = 3.8 EE64 pKa = 5.01 LSDD67 pKa = 3.72 KK68 pKa = 11.24 LEE70 pKa = 4.43 NGEE73 pKa = 4.17 DD74 pKa = 3.52 MMVYY78 pKa = 10.05 FYY80 pKa = 11.25 DD81 pKa = 3.96 PTCPHH86 pKa = 6.31 CQEE89 pKa = 3.98 LTPRR93 pKa = 11.84 LVPIAEE99 pKa = 4.36 DD100 pKa = 3.4 MNVDD104 pKa = 3.65 MKK106 pKa = 11.12 KK107 pKa = 10.86 LNLLEE112 pKa = 5.3 FQGAWNTYY120 pKa = 9.16 GIQSTPTLVYY130 pKa = 10.52 FEE132 pKa = 5.7 DD133 pKa = 4.58 GEE135 pKa = 4.42 EE136 pKa = 3.82 VDD138 pKa = 5.31 RR139 pKa = 11.84 INGAQQNEE147 pKa = 4.25 LFEE150 pKa = 5.34 AFFNEE155 pKa = 4.19 YY156 pKa = 10.59 AADD159 pKa = 4.06 DD160 pKa = 4.13 SGDD163 pKa = 3.73 EE164 pKa = 4.15 QEE166 pKa = 5.43 AGAA169 pKa = 4.85
Molecular weight: 19.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.605
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A1I1UQ82|A0A1I1UQ82_9BACI 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Lentibacillus persicus OX=640948 GN=ispE PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.49 KK14 pKa = 8.63 VHH16 pKa = 5.7 GFRR19 pKa = 11.84 KK20 pKa = 9.73 RR21 pKa = 11.84 MKK23 pKa = 10.42 SKK25 pKa = 10.78 DD26 pKa = 2.93 GRR28 pKa = 11.84 QVLKK32 pKa = 10.25 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.96 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 10.935
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.471
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.05
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3300
0
3300
945704
25
1614
286.6
32.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.488 ± 0.045
0.6 ± 0.012
5.722 ± 0.038
7.555 ± 0.054
4.394 ± 0.033
7.082 ± 0.041
2.137 ± 0.022
7.488 ± 0.042
6.178 ± 0.041
9.336 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.958 ± 0.02
4.575 ± 0.029
3.691 ± 0.025
3.748 ± 0.028
4.085 ± 0.028
5.953 ± 0.03
5.542 ± 0.025
7.001 ± 0.037
1.01 ± 0.015
3.457 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here