Dunaliella viridis virus SI2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QBZ4|M4QBZ4_9CAUD Uncharacterized protein OS=Dunaliella viridis virus SI2 OX=754069 GN=DVVG_00048 PE=4 SV=1
MM1 pKa = 7.11TVDD4 pKa = 4.19PFSEE8 pKa = 4.12LMDD11 pKa = 3.76MASDD15 pKa = 3.66PLFRR19 pKa = 11.84DD20 pKa = 3.36QVSGVGSEE28 pKa = 4.37VEE30 pKa = 4.35VNDD33 pKa = 4.03TGLDD37 pKa = 3.43NAIYY41 pKa = 9.23LACFATPAGRR51 pKa = 11.84AVLADD56 pKa = 4.05LYY58 pKa = 11.44NRR60 pKa = 11.84FVNGTRR66 pKa = 11.84FEE68 pKa = 4.76PGQDD72 pKa = 3.37PSIGYY77 pKa = 8.76YY78 pKa = 9.68RR79 pKa = 11.84EE80 pKa = 3.82GGAYY84 pKa = 9.94VVFDD88 pKa = 3.4IAARR92 pKa = 11.84IEE94 pKa = 3.84AAAQGDD100 pKa = 3.98EE101 pKa = 5.17EE102 pKa = 4.47EE103 pKa = 4.65TQDD106 pKa = 4.05AQQDD110 pKa = 3.44

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4Q0Z0|M4Q0Z0_9CAUD Uncharacterized protein OS=Dunaliella viridis virus SI2 OX=754069 GN=DVVG_00012 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.46NKK5 pKa = 10.38VHH7 pKa = 6.61LQPMHH12 pKa = 6.63APWRR16 pKa = 11.84DD17 pKa = 3.19HH18 pKa = 6.65VNAEE22 pKa = 3.91EE23 pKa = 4.05KK24 pKa = 10.41QRR26 pKa = 11.84LDD28 pKa = 3.27VLEE31 pKa = 3.91QRR33 pKa = 11.84LSRR36 pKa = 11.84KK37 pKa = 9.4RR38 pKa = 11.84ATVEE42 pKa = 3.88DD43 pKa = 4.44LVSEE47 pKa = 3.91QHH49 pKa = 6.56RR50 pKa = 11.84IMRR53 pKa = 11.84RR54 pKa = 11.84AIARR58 pKa = 11.84MRR60 pKa = 11.84RR61 pKa = 11.84KK62 pKa = 9.44EE63 pKa = 4.16GKK65 pKa = 9.64QKK67 pKa = 11.1

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11495

67

919

225.4

24.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.084 ± 0.644

0.766 ± 0.158

6.49 ± 0.277

6.986 ± 0.364

3.654 ± 0.23

8.752 ± 0.315

1.705 ± 0.189

4.689 ± 0.159

2.636 ± 0.339

7.673 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.173

3.08 ± 0.163

4.776 ± 0.298

4.037 ± 0.189

7.969 ± 0.391

5.689 ± 0.266

6.203 ± 0.495

6.246 ± 0.29

1.435 ± 0.176

2.279 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski