Flavobacterium phage FL-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ficleduovirus; unclassified Ficleduovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8VWA1|A0A1P8VWA1_9CAUD Uncharacterized protein OS=Flavobacterium phage FL-1 OX=908820 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TIKK5 pKa = 10.66NLAVPQNSDD14 pKa = 3.15PKK16 pKa = 10.52FPYY19 pKa = 9.22STILNEE25 pKa = 3.87TDD27 pKa = 3.56TNDD30 pKa = 3.26GTPVVEE36 pKa = 5.13EE37 pKa = 4.28IYY39 pKa = 11.34GDD41 pKa = 3.47ILTNNYY47 pKa = 10.09KK48 pKa = 10.58LLQTVDD54 pKa = 4.22IIPTGTQDD62 pKa = 4.59SDD64 pKa = 3.51DD65 pKa = 4.22DD66 pKa = 3.85QYY68 pKa = 11.98QILDD72 pKa = 3.91ALQLLPNKK80 pKa = 9.73TNDD83 pKa = 3.1IEE85 pKa = 4.45QVLSLDD91 pKa = 3.51SGIWSVPLKK100 pKa = 10.71IEE102 pKa = 3.95ILPNKK107 pKa = 10.09YY108 pKa = 10.16FFLARR113 pKa = 11.84AAEE116 pKa = 4.04NYY118 pKa = 9.69IVGSSYY124 pKa = 9.31TFKK127 pKa = 10.74GTGTTTYY134 pKa = 9.55PFSSLGFNSGDD145 pKa = 3.67EE146 pKa = 3.79ILIIIDD152 pKa = 3.4QSGVRR157 pKa = 11.84AYY159 pKa = 11.25SLVSQAATASEE170 pKa = 4.42IFTVMGTPVSFNDD183 pKa = 3.99SNKK186 pKa = 9.26VWYY189 pKa = 9.53QDD191 pKa = 3.29SGSLMSDD198 pKa = 3.51MPSTAYY204 pKa = 10.46LEE206 pKa = 4.41SIIRR210 pKa = 11.84VDD212 pKa = 3.55VSDD215 pKa = 3.82GTVLLNDD222 pKa = 3.47ILISNGYY229 pKa = 8.8ALCFCLVPGTNTYY242 pKa = 10.07FFRR245 pKa = 11.84QFLLSDD251 pKa = 4.17LTLSTSVTISGTSFANSSDD270 pKa = 3.44FSPYY274 pKa = 10.02VYY276 pKa = 10.59AEE278 pKa = 3.84SGIVYY283 pKa = 7.6VTNAMNTTANNYY295 pKa = 10.41SLTKK299 pKa = 10.62LIYY302 pKa = 9.45NAGAATLTFSSTISLDD318 pKa = 3.23NTFVKK323 pKa = 8.09TTNAAIKK330 pKa = 10.5SGLLYY335 pKa = 10.76TMISGALNSFNLVGGTKK352 pKa = 10.15VSLGNYY358 pKa = 9.42LGIAGHH364 pKa = 6.61IFSFNGFIYY373 pKa = 10.34FSSGEE378 pKa = 4.0VAKK381 pKa = 10.54RR382 pKa = 11.84WFF384 pKa = 3.57

Molecular weight: 41.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8VWG8|A0A1P8VWG8_9CAUD Uncharacterized protein OS=Flavobacterium phage FL-1 OX=908820 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84SRR4 pKa = 11.84ARR6 pKa = 11.84KK7 pKa = 8.44PSEE10 pKa = 3.56VQIAIKK16 pKa = 10.3GVYY19 pKa = 9.51LFLSAVVVLTIILNN33 pKa = 3.73

Molecular weight: 3.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15873

33

680

182.4

20.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.483 ± 0.42

0.806 ± 0.092

5.664 ± 0.22

6.848 ± 0.425

4.725 ± 0.168

6.287 ± 0.32

1.323 ± 0.119

7.913 ± 0.238

8.474 ± 0.486

8.253 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.153

6.319 ± 0.334

3.049 ± 0.208

3.478 ± 0.186

3.553 ± 0.173

7.163 ± 0.217

6.275 ± 0.333

6.224 ± 0.237

1.121 ± 0.113

3.698 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski