Spiribacter sp. E85

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Spiribacter; unclassified Spiribacter

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U2MX23|A0A2U2MX23_9GAMM 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB OS=Spiribacter sp. E85 OX=2182432 GN=rlmB PE=3 SV=1
MM1 pKa = 6.92MKK3 pKa = 8.91KK4 pKa = 7.86TASALAVAALMGTGAASAATFQVNDD29 pKa = 3.74DD30 pKa = 4.21TTLSLYY36 pKa = 11.17GNVQIAYY43 pKa = 10.1SDD45 pKa = 3.83TNDD48 pKa = 3.14ASGDD52 pKa = 4.23SVSDD56 pKa = 3.64LQDD59 pKa = 3.12NGTTIGVAVEE69 pKa = 3.96HH70 pKa = 6.76RR71 pKa = 11.84FDD73 pKa = 3.73NGLTGFARR81 pKa = 11.84IEE83 pKa = 3.95EE84 pKa = 4.48DD85 pKa = 3.74GYY87 pKa = 11.63DD88 pKa = 3.64AVEE91 pKa = 4.19QAAEE95 pKa = 4.01TAATDD100 pKa = 3.29QAYY103 pKa = 10.2FGVRR107 pKa = 11.84GDD109 pKa = 3.61FGMVRR114 pKa = 11.84LGNADD119 pKa = 3.76SIYY122 pKa = 10.79DD123 pKa = 3.77GFVRR127 pKa = 11.84DD128 pKa = 3.46YY129 pKa = 10.88WDD131 pKa = 3.42YY132 pKa = 11.86QEE134 pKa = 5.71FLAPINGRR142 pKa = 11.84RR143 pKa = 11.84GVDD146 pKa = 2.98HH147 pKa = 7.95KK148 pKa = 11.22DD149 pKa = 3.16RR150 pKa = 11.84QLLYY154 pKa = 9.64MSPNFGGFSFGLEE167 pKa = 3.77AQINGDD173 pKa = 3.27ADD175 pKa = 3.55EE176 pKa = 4.76ATFEE180 pKa = 4.19LTQGGVNNGDD190 pKa = 3.75NEE192 pKa = 4.49TGDD195 pKa = 3.83VNGDD199 pKa = 3.49DD200 pKa = 5.0AGISLAAAAMYY211 pKa = 7.58EE212 pKa = 4.33TGGLTVSAAIDD223 pKa = 3.35QRR225 pKa = 11.84SNEE228 pKa = 4.17FVSAADD234 pKa = 3.77AGSEE238 pKa = 3.95GGPNHH243 pKa = 6.7FDD245 pKa = 3.61DD246 pKa = 4.6PVMGIVVSYY255 pKa = 10.97DD256 pKa = 3.18FGRR259 pKa = 11.84FTVDD263 pKa = 3.43GGYY266 pKa = 10.13QLDD269 pKa = 4.06AADD272 pKa = 3.75TDD274 pKa = 3.57EE275 pKa = 4.75RR276 pKa = 11.84AIGDD280 pKa = 3.71VDD282 pKa = 3.87VLSLGGRR289 pKa = 11.84FNYY292 pKa = 10.54GMGDD296 pKa = 3.54LYY298 pKa = 11.36GIVQEE303 pKa = 4.28VGYY306 pKa = 10.63DD307 pKa = 3.56EE308 pKa = 5.39EE309 pKa = 4.33NAAGNDD315 pKa = 3.33SFTEE319 pKa = 4.51VILGANYY326 pKa = 10.45NIGSNFYY333 pKa = 10.85VYY335 pKa = 10.9AEE337 pKa = 4.17YY338 pKa = 8.63ATYY341 pKa = 10.41DD342 pKa = 3.53QEE344 pKa = 4.5EE345 pKa = 4.25DD346 pKa = 3.79ANDD349 pKa = 3.5GFAIGGLYY357 pKa = 10.55SFF359 pKa = 5.56

Molecular weight: 38.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U2N2X6|A0A2U2N2X6_9GAMM Probable chromosome-partitioning protein ParB OS=Spiribacter sp. E85 OX=2182432 GN=DEM34_08705 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.32GRR39 pKa = 11.84ARR41 pKa = 11.84LTPP44 pKa = 3.93

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3732

0

3732

1191587

33

2193

319.3

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.168 ± 0.065

0.91 ± 0.011

5.536 ± 0.034

6.798 ± 0.045

3.129 ± 0.028

9.074 ± 0.042

2.263 ± 0.019

3.899 ± 0.031

1.563 ± 0.025

11.325 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.031 ± 0.019

1.94 ± 0.021

5.548 ± 0.03

3.148 ± 0.023

9.16 ± 0.045

4.543 ± 0.028

4.727 ± 0.02

7.602 ± 0.03

1.437 ± 0.018

2.2 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski