Streptomyces hoynatensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9Z7F1|A0A3A9Z7F1_9ACTN Thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha OS=Streptomyces hoynatensis OX=1141874 GN=D7294_11780 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84KK3 pKa = 8.71PVLALCTATALLAGAVGCSSDD24 pKa = 4.73DD25 pKa = 4.16RR26 pKa = 11.84PDD28 pKa = 3.02QRR30 pKa = 11.84APSEE34 pKa = 4.37SPSGSEE40 pKa = 3.44QTPPPEE46 pKa = 5.69APDD49 pKa = 3.85APVLEE54 pKa = 4.79ALPEE58 pKa = 4.43EE59 pKa = 4.94IPAEE63 pKa = 3.99LDD65 pKa = 3.27PYY67 pKa = 11.28YY68 pKa = 10.16EE69 pKa = 4.45QQLSWQPCEE78 pKa = 5.0AGFEE82 pKa = 4.4CATMRR87 pKa = 11.84VPLDD91 pKa = 3.77YY92 pKa = 11.47EE93 pKa = 4.51NVDD96 pKa = 3.42PDD98 pKa = 3.4QDD100 pKa = 4.07LEE102 pKa = 4.44LTVTRR107 pKa = 11.84SQATDD112 pKa = 3.42PSSRR116 pKa = 11.84IGSLLVNPGGPGASAVDD133 pKa = 4.36FAQDD137 pKa = 3.0SASYY141 pKa = 9.98IFSSEE146 pKa = 4.05VTSHH150 pKa = 6.3YY151 pKa = 11.14DD152 pKa = 3.28VVAVDD157 pKa = 3.74PRR159 pKa = 11.84GTGASEE165 pKa = 3.97PVQCMSGEE173 pKa = 4.09QMDD176 pKa = 4.63EE177 pKa = 3.8FTLNDD182 pKa = 4.11RR183 pKa = 11.84TPDD186 pKa = 3.55SQDD189 pKa = 3.65EE190 pKa = 4.25IDD192 pKa = 3.94TLLSSFDD199 pKa = 3.57EE200 pKa = 4.92FAASCAEE207 pKa = 3.89HH208 pKa = 7.26SGDD211 pKa = 4.04LLGHH215 pKa = 6.85ISTIEE220 pKa = 3.97SARR223 pKa = 11.84DD224 pKa = 3.4MDD226 pKa = 4.31VLRR229 pKa = 11.84ALLGDD234 pKa = 4.09DD235 pKa = 3.17QLHH238 pKa = 5.41YY239 pKa = 11.35VGFSYY244 pKa = 8.91GTKK247 pKa = 10.3LGAVYY252 pKa = 10.54AGLFPQRR259 pKa = 11.84VGRR262 pKa = 11.84LVLDD266 pKa = 4.06GAIDD270 pKa = 3.77PRR272 pKa = 11.84FPTLDD277 pKa = 3.43TDD279 pKa = 3.94RR280 pKa = 11.84QQAGGFEE287 pKa = 4.04TAFRR291 pKa = 11.84SFAEE295 pKa = 4.62DD296 pKa = 3.36CASRR300 pKa = 11.84PDD302 pKa = 4.34CPLGTQGGDD311 pKa = 3.39DD312 pKa = 3.41ASQRR316 pKa = 11.84LLDD319 pKa = 4.11FFEE322 pKa = 4.5QVDD325 pKa = 4.26AEE327 pKa = 4.38PLPTSDD333 pKa = 4.02PDD335 pKa = 3.57RR336 pKa = 11.84PLTEE340 pKa = 4.42SLASTGVSMAMYY352 pKa = 10.15SDD354 pKa = 4.03YY355 pKa = 10.36MWEE358 pKa = 4.0QLRR361 pKa = 11.84EE362 pKa = 4.11ALRR365 pKa = 11.84AAISQGNGDD374 pKa = 4.13QLLALADD381 pKa = 4.27EE382 pKa = 4.76YY383 pKa = 11.66NSRR386 pKa = 11.84EE387 pKa = 3.77PDD389 pKa = 3.22GSYY392 pKa = 9.57GTDD395 pKa = 2.68MFAFPAISCLDD406 pKa = 3.71SPAGNATEE414 pKa = 5.46DD415 pKa = 3.75EE416 pKa = 4.51VEE418 pKa = 4.39EE419 pKa = 3.91NLPAYY424 pKa = 9.82EE425 pKa = 4.1EE426 pKa = 4.32ASPTFGRR433 pKa = 11.84DD434 pKa = 3.36FAWATLQCAAWPVEE448 pKa = 4.11PTGAPVTIEE457 pKa = 4.12AEE459 pKa = 4.17GANDD463 pKa = 3.14IVVVGTTRR471 pKa = 11.84DD472 pKa = 3.27PATPYY477 pKa = 10.63SWAQGLADD485 pKa = 3.72QLSSGVLLTYY495 pKa = 10.84DD496 pKa = 3.89GDD498 pKa = 3.53GHH500 pKa = 5.35TAYY503 pKa = 10.24GGTSPCIDD511 pKa = 3.52AAVNAYY517 pKa = 10.02LLEE520 pKa = 4.47GQTPEE525 pKa = 4.85EE526 pKa = 4.6GTTCC530 pKa = 4.53

Molecular weight: 56.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9YNW6|A0A3A9YNW6_9ACTN Polyketide cyclase OS=Streptomyces hoynatensis OX=1141874 GN=D7294_29040 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.82GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5959

0

5959

2017824

18

9676

338.6

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.894 ± 0.057

0.797 ± 0.009

5.406 ± 0.028

6.259 ± 0.032

2.648 ± 0.019

10.163 ± 0.032

2.258 ± 0.014

2.736 ± 0.024

1.33 ± 0.018

10.856 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.547 ± 0.013

1.56 ± 0.016

6.608 ± 0.037

2.606 ± 0.02

8.912 ± 0.042

4.604 ± 0.023

5.463 ± 0.026

7.871 ± 0.032

1.529 ± 0.012

1.954 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski