Pseudomonas phage phiIBB-PF7A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pifdecavirus; Pseudomonas virus IBBPF7A

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9KII3|E9KII3_9CAUD Uncharacterized protein OS=Pseudomonas phage phiIBB-PF7A OX=942165 GN=phiIBB-PF7Ap43 PE=4 SV=1
MM1 pKa = 7.55ARR3 pKa = 11.84LDD5 pKa = 3.46MHH7 pKa = 5.74VTGLEE12 pKa = 3.85YY13 pKa = 10.76DD14 pKa = 3.89LSLEE18 pKa = 4.23EE19 pKa = 4.35NAQGQQGFTVTYY31 pKa = 8.56GAQVNHH37 pKa = 5.94YY38 pKa = 9.65EE39 pKa = 4.17RR40 pKa = 11.84FDD42 pKa = 3.58EE43 pKa = 5.49AFMDD47 pKa = 4.37FNEE50 pKa = 4.57SLSHH54 pKa = 7.02ALALQGLL61 pKa = 4.22

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9KIF6|E9KIF6_9CAUD Uncharacterized protein OS=Pseudomonas phage phiIBB-PF7A OX=942165 GN=phiIBB-PF7Ap18 PE=4 SV=1
MM1 pKa = 7.69AIKK4 pKa = 9.37VTLKK8 pKa = 9.96QLRR11 pKa = 11.84NFDD14 pKa = 3.98LVQALLAAAGRR25 pKa = 11.84VHH27 pKa = 7.63AKK29 pKa = 9.97RR30 pKa = 11.84LARR33 pKa = 11.84LKK35 pKa = 10.81ARR37 pKa = 11.84EE38 pKa = 4.12EE39 pKa = 3.97ALKK42 pKa = 9.82ATINAATVALVEE54 pKa = 4.32TQKK57 pKa = 10.75NRR59 pKa = 11.84IDD61 pKa = 3.49HH62 pKa = 5.77QFADD66 pKa = 3.58LRR68 pKa = 11.84VVVKK72 pKa = 10.74

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12904

45

1331

258.1

28.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.059 ± 0.539

0.814 ± 0.136

6.564 ± 0.206

6.556 ± 0.345

3.65 ± 0.163

8.16 ± 0.357

2.255 ± 0.17

4.533 ± 0.167

5.874 ± 0.335

8.176 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.139 ± 0.23

3.805 ± 0.182

3.906 ± 0.159

4.363 ± 0.338

5.882 ± 0.211

5.401 ± 0.281

5.673 ± 0.239

6.951 ± 0.39

1.317 ± 0.151

2.922 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski