Amino acid dipepetide frequency for Sphingomonas sp. KC8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.831AlaAla: 20.831 ± 0.216
1.015AlaCys: 1.015 ± 0.03
8.566AlaAsp: 8.566 ± 0.107
7.639AlaGlu: 7.639 ± 0.099
4.422AlaPhe: 4.422 ± 0.067
12.382AlaGly: 12.382 ± 0.135
2.433AlaHis: 2.433 ± 0.054
7.729AlaIle: 7.729 ± 0.086
4.012AlaLys: 4.012 ± 0.071
13.895AlaLeu: 13.895 ± 0.151
4.09AlaMet: 4.09 ± 0.056
3.209AlaAsn: 3.209 ± 0.059
6.522AlaPro: 6.522 ± 0.089
4.308AlaGln: 4.308 ± 0.064
10.274AlaArg: 10.274 ± 0.13
6.514AlaSer: 6.514 ± 0.084
7.21AlaThr: 7.21 ± 0.088
9.055AlaVal: 9.055 ± 0.105
1.703AlaTrp: 1.703 ± 0.04
2.709AlaTyr: 2.709 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.966CysAla: 0.966 ± 0.024
0.085CysCys: 0.085 ± 0.008
0.514CysAsp: 0.514 ± 0.022
0.349CysGlu: 0.349 ± 0.019
0.27CysPhe: 0.27 ± 0.014
0.868CysGly: 0.868 ± 0.029
0.262CysHis: 0.262 ± 0.019
0.327CysIle: 0.327 ± 0.016
0.154CysLys: 0.154 ± 0.012
0.676CysLeu: 0.676 ± 0.027
0.145CysMet: 0.145 ± 0.011
0.184CysAsn: 0.184 ± 0.012
0.487CysPro: 0.487 ± 0.021
0.197CysGln: 0.197 ± 0.014
0.555CysArg: 0.555 ± 0.023
0.415CysSer: 0.415 ± 0.018
0.371CysThr: 0.371 ± 0.016
0.536CysVal: 0.536 ± 0.022
0.093CysTrp: 0.093 ± 0.009
0.177CysTyr: 0.177 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.399AspAla: 8.399 ± 0.094
0.507AspCys: 0.507 ± 0.019
3.523AspAsp: 3.523 ± 0.075
3.36AspGlu: 3.36 ± 0.061
2.159AspPhe: 2.159 ± 0.044
6.116AspGly: 6.116 ± 0.078
1.382AspHis: 1.382 ± 0.037
3.137AspIle: 3.137 ± 0.051
1.653AspLys: 1.653 ± 0.042
5.648AspLeu: 5.648 ± 0.067
1.436AspMet: 1.436 ± 0.036
1.36AspAsn: 1.36 ± 0.041
3.838AspPro: 3.838 ± 0.068
1.891AspGln: 1.891 ± 0.042
5.196AspArg: 5.196 ± 0.067
2.276AspSer: 2.276 ± 0.044
2.579AspThr: 2.579 ± 0.051
4.292AspVal: 4.292 ± 0.059
1.106AspTrp: 1.106 ± 0.031
1.674AspTyr: 1.674 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
7.238GluAla: 7.238 ± 0.095
0.335GluCys: 0.335 ± 0.015
2.508GluAsp: 2.508 ± 0.049
2.397GluGlu: 2.397 ± 0.053
1.485GluPhe: 1.485 ± 0.037
4.142GluGly: 4.142 ± 0.059
1.026GluHis: 1.026 ± 0.033
3.002GluIle: 3.002 ± 0.057
1.788GluLys: 1.788 ± 0.044
4.777GluLeu: 4.777 ± 0.066
1.409GluMet: 1.409 ± 0.036
1.334GluAsn: 1.334 ± 0.034
2.45GluPro: 2.45 ± 0.048
1.921GluGln: 1.921 ± 0.038
4.649GluArg: 4.649 ± 0.075
2.062GluSer: 2.062 ± 0.04
3.083GluThr: 3.083 ± 0.049
3.278GluVal: 3.278 ± 0.055
0.727GluTrp: 0.727 ± 0.025
0.994GluTyr: 0.994 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.787PheAla: 4.787 ± 0.071
0.31PheCys: 0.31 ± 0.015
2.758PheAsp: 2.758 ± 0.053
1.904PheGlu: 1.904 ± 0.044
1.192PhePhe: 1.192 ± 0.034
3.725PheGly: 3.725 ± 0.068
0.747PheHis: 0.747 ± 0.027
1.48PheIle: 1.48 ± 0.035
0.842PheLys: 0.842 ± 0.026
2.961PheLeu: 2.961 ± 0.061
0.728PheMet: 0.728 ± 0.024
1.047PheAsn: 1.047 ± 0.035
1.508PhePro: 1.508 ± 0.034
0.933PheGln: 0.933 ± 0.024
2.193PheArg: 2.193 ± 0.047
1.977PheSer: 1.977 ± 0.045
2.072PheThr: 2.072 ± 0.042
2.54PheVal: 2.54 ± 0.048
0.466PheTrp: 0.466 ± 0.022
0.852PheTyr: 0.852 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.498GlyAla: 10.498 ± 0.131
0.845GlyCys: 0.845 ± 0.032
5.256GlyAsp: 5.256 ± 0.072
4.545GlyGlu: 4.545 ± 0.059
3.733GlyPhe: 3.733 ± 0.057
8.915GlyGly: 8.915 ± 0.158
2.032GlyHis: 2.032 ± 0.047
4.641GlyIle: 4.641 ± 0.063
3.08GlyLys: 3.08 ± 0.05
8.924GlyLeu: 8.924 ± 0.092
2.381GlyMet: 2.381 ± 0.044
2.327GlyAsn: 2.327 ± 0.065
3.726GlyPro: 3.726 ± 0.056
3.139GlyGln: 3.139 ± 0.057
6.892GlyArg: 6.892 ± 0.076
4.694GlySer: 4.694 ± 0.098
4.901GlyThr: 4.901 ± 0.126
6.546GlyVal: 6.546 ± 0.081
1.703GlyTrp: 1.703 ± 0.036
2.369GlyTyr: 2.369 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.493HisAla: 2.493 ± 0.053
0.198HisCys: 0.198 ± 0.012
1.243HisAsp: 1.243 ± 0.034
0.896HisGlu: 0.896 ± 0.025
0.87HisPhe: 0.87 ± 0.028
2.065HisGly: 2.065 ± 0.047
0.601HisHis: 0.601 ± 0.027
1.023HisIle: 1.023 ± 0.029
0.446HisLys: 0.446 ± 0.019
1.932HisLeu: 1.932 ± 0.039
0.515HisMet: 0.515 ± 0.018
0.468HisAsn: 0.468 ± 0.021
1.294HisPro: 1.294 ± 0.033
0.579HisGln: 0.579 ± 0.023
1.53HisArg: 1.53 ± 0.041
0.921HisSer: 0.921 ± 0.028
0.735HisThr: 0.735 ± 0.029
1.559HisVal: 1.559 ± 0.036
0.328HisTrp: 0.328 ± 0.017
0.558HisTyr: 0.558 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
8.708IleAla: 8.708 ± 0.081
0.432IleCys: 0.432 ± 0.017
4.19IleAsp: 4.19 ± 0.06
3.586IleGlu: 3.586 ± 0.056
1.598IlePhe: 1.598 ± 0.037
5.594IleGly: 5.594 ± 0.073
1.018IleHis: 1.018 ± 0.028
2.331IleIle: 2.331 ± 0.046
1.145IleLys: 1.145 ± 0.035
4.169IleLeu: 4.169 ± 0.07
0.899IleMet: 0.899 ± 0.032
1.433IleAsn: 1.433 ± 0.047
2.176IlePro: 2.176 ± 0.046
1.208IleGln: 1.208 ± 0.028
3.47IleArg: 3.47 ± 0.063
2.613IleSer: 2.613 ± 0.049
2.753IleThr: 2.753 ± 0.047
4.323IleVal: 4.323 ± 0.065
0.611IleTrp: 0.611 ± 0.023
1.018IleTyr: 1.018 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
4.169LysAla: 4.169 ± 0.062
0.145LysCys: 0.145 ± 0.01
1.543LysAsp: 1.543 ± 0.048
1.152LysGlu: 1.152 ± 0.035
0.781LysPhe: 0.781 ± 0.027
2.46LysGly: 2.46 ± 0.045
0.497LysHis: 0.497 ± 0.017
1.422LysIle: 1.422 ± 0.04
0.957LysLys: 0.957 ± 0.03
2.988LysLeu: 2.988 ± 0.055
0.733LysMet: 0.733 ± 0.028
0.675LysAsn: 0.675 ± 0.023
1.868LysPro: 1.868 ± 0.046
0.863LysGln: 0.863 ± 0.03
2.098LysArg: 2.098 ± 0.044
1.517LysSer: 1.517 ± 0.039
1.578LysThr: 1.578 ± 0.031
2.097LysVal: 2.097 ± 0.049
0.35LysTrp: 0.35 ± 0.018
0.59LysTyr: 0.59 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
14.384LeuAla: 14.384 ± 0.143
0.786LeuCys: 0.786 ± 0.023
6.311LeuAsp: 6.311 ± 0.075
4.339LeuGlu: 4.339 ± 0.066
3.518LeuPhe: 3.518 ± 0.066
8.288LeuGly: 8.288 ± 0.096
1.851LeuHis: 1.851 ± 0.042
5.239LeuIle: 5.239 ± 0.072
2.96LeuLys: 2.96 ± 0.051
8.914LeuLeu: 8.914 ± 0.106
2.218LeuMet: 2.218 ± 0.042
2.43LeuAsn: 2.43 ± 0.055
5.402LeuPro: 5.402 ± 0.068
2.37LeuGln: 2.37 ± 0.049
6.675LeuArg: 6.675 ± 0.09
5.535LeuSer: 5.535 ± 0.06
5.348LeuThr: 5.348 ± 0.08
7.022LeuVal: 7.022 ± 0.091
1.191LeuTrp: 1.191 ± 0.031
1.89LeuTyr: 1.89 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.598MetAla: 3.598 ± 0.061
0.148MetCys: 0.148 ± 0.01
1.122MetAsp: 1.122 ± 0.032
1.008MetGlu: 1.008 ± 0.029
0.698MetPhe: 0.698 ± 0.024
1.938MetGly: 1.938 ± 0.044
0.44MetHis: 0.44 ± 0.02
1.44MetIle: 1.44 ± 0.038
0.9MetLys: 0.9 ± 0.028
2.743MetLeu: 2.743 ± 0.052
0.675MetMet: 0.675 ± 0.026
0.684MetAsn: 0.684 ± 0.023
1.538MetPro: 1.538 ± 0.036
0.741MetGln: 0.741 ± 0.023
1.838MetArg: 1.838 ± 0.035
1.333MetSer: 1.333 ± 0.03
1.759MetThr: 1.759 ± 0.037
1.643MetVal: 1.643 ± 0.037
0.254MetTrp: 0.254 ± 0.016
0.275MetTyr: 0.275 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.269AsnAla: 3.269 ± 0.056
0.202AsnCys: 0.202 ± 0.014
1.437AsnAsp: 1.437 ± 0.047
1.052AsnGlu: 1.052 ± 0.032
0.926AsnPhe: 0.926 ± 0.033
2.582AsnGly: 2.582 ± 0.073
0.461AsnHis: 0.461 ± 0.02
1.412AsnIle: 1.412 ± 0.041
0.605AsnLys: 0.605 ± 0.023
2.439AsnLeu: 2.439 ± 0.049
0.586AsnMet: 0.586 ± 0.023
0.71AsnAsn: 0.71 ± 0.028
1.752AsnPro: 1.752 ± 0.036
0.736AsnGln: 0.736 ± 0.025
1.829AsnArg: 1.829 ± 0.042
1.223AsnSer: 1.223 ± 0.035
1.061AsnThr: 1.061 ± 0.031
1.927AsnVal: 1.927 ± 0.041
0.398AsnTrp: 0.398 ± 0.018
0.696AsnTyr: 0.696 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
7.615ProAla: 7.615 ± 0.09
0.318ProCys: 0.318 ± 0.015
3.822ProAsp: 3.822 ± 0.055
3.175ProGlu: 3.175 ± 0.054
1.957ProPhe: 1.957 ± 0.042
4.904ProGly: 4.904 ± 0.057
1.104ProHis: 1.104 ± 0.03
2.654ProIle: 2.654 ± 0.041
1.448ProLys: 1.448 ± 0.032
4.754ProLeu: 4.754 ± 0.071
1.288ProMet: 1.288 ± 0.033
1.294ProAsn: 1.294 ± 0.03
2.888ProPro: 2.888 ± 0.07
1.608ProGln: 1.608 ± 0.029
2.978ProArg: 2.978 ± 0.057
2.602ProSer: 2.602 ± 0.044
2.605ProThr: 2.605 ± 0.046
4.324ProVal: 4.324 ± 0.058
0.719ProTrp: 0.719 ± 0.026
1.04ProTyr: 1.04 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
4.322GlnAla: 4.322 ± 0.068
0.208GlnCys: 0.208 ± 0.014
1.403GlnAsp: 1.403 ± 0.038
1.182GlnGlu: 1.182 ± 0.033
1.089GlnPhe: 1.089 ± 0.029
2.461GlnGly: 2.461 ± 0.047
0.605GlnHis: 0.605 ± 0.024
1.8GlnIle: 1.8 ± 0.029
0.905GlnLys: 0.905 ± 0.026
2.849GlnLeu: 2.849 ± 0.051
0.833GlnMet: 0.833 ± 0.026
0.739GlnAsn: 0.739 ± 0.027
1.878GlnPro: 1.878 ± 0.039
1.104GlnGln: 1.104 ± 0.036
2.409GlnArg: 2.409 ± 0.043
1.596GlnSer: 1.596 ± 0.039
1.618GlnThr: 1.618 ± 0.036
2.129GlnVal: 2.129 ± 0.047
0.398GlnTrp: 0.398 ± 0.019
0.612GlnTyr: 0.612 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
9.397ArgAla: 9.397 ± 0.102
0.493ArgCys: 0.493 ± 0.021
4.454ArgAsp: 4.454 ± 0.065
3.735ArgGlu: 3.735 ± 0.062
3.061ArgPhe: 3.061 ± 0.056
5.135ArgGly: 5.135 ± 0.062
1.687ArgHis: 1.687 ± 0.04
4.451ArgIle: 4.451 ± 0.057
1.936ArgLys: 1.936 ± 0.044
8.399ArgLeu: 8.399 ± 0.105
2.024ArgMet: 2.024 ± 0.036
1.886ArgAsn: 1.886 ± 0.042
3.904ArgPro: 3.904 ± 0.058
2.428ArgGln: 2.428 ± 0.044
5.83ArgArg: 5.83 ± 0.089
3.496ArgSer: 3.496 ± 0.052
3.682ArgThr: 3.682 ± 0.06
4.776ArgVal: 4.776 ± 0.06
1.234ArgTrp: 1.234 ± 0.034
1.82ArgTyr: 1.82 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.402SerAla: 6.402 ± 0.078
0.362SerCys: 0.362 ± 0.018
2.944SerAsp: 2.944 ± 0.056
2.29SerGlu: 2.29 ± 0.05
2.079SerPhe: 2.079 ± 0.043
5.381SerGly: 5.381 ± 0.088
0.976SerHis: 0.976 ± 0.029
2.732SerIle: 2.732 ± 0.054
1.32SerLys: 1.32 ± 0.032
4.794SerLeu: 4.794 ± 0.055
1.114SerMet: 1.114 ± 0.03
1.266SerAsn: 1.266 ± 0.034
2.746SerPro: 2.746 ± 0.048
1.436SerGln: 1.436 ± 0.038
3.304SerArg: 3.304 ± 0.053
2.552SerSer: 2.552 ± 0.06
2.593SerThr: 2.593 ± 0.057
3.474SerVal: 3.474 ± 0.054
0.681SerTrp: 0.681 ± 0.022
1.294SerTyr: 1.294 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
6.871ThrAla: 6.871 ± 0.084
0.376ThrCys: 0.376 ± 0.018
2.891ThrAsp: 2.891 ± 0.053
2.216ThrGlu: 2.216 ± 0.041
1.779ThrPhe: 1.779 ± 0.042
5.488ThrGly: 5.488 ± 0.099
0.928ThrHis: 0.928 ± 0.026
3.105ThrIle: 3.105 ± 0.051
1.313ThrLys: 1.313 ± 0.035
5.886ThrLeu: 5.886 ± 0.105
1.205ThrMet: 1.205 ± 0.035
1.334ThrAsn: 1.334 ± 0.039
3.43ThrPro: 3.43 ± 0.055
1.395ThrGln: 1.395 ± 0.032
3.462ThrArg: 3.462 ± 0.055
2.631ThrSer: 2.631 ± 0.05
2.741ThrThr: 2.741 ± 0.062
4.142ThrVal: 4.142 ± 0.062
0.587ThrTrp: 0.587 ± 0.023
1.152ThrTyr: 1.152 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
10.311ValAla: 10.311 ± 0.105
0.519ValCys: 0.519 ± 0.019
4.814ValAsp: 4.814 ± 0.065
4.177ValGlu: 4.177 ± 0.065
2.024ValPhe: 2.024 ± 0.045
5.602ValGly: 5.602 ± 0.071
1.379ValHis: 1.379 ± 0.033
3.788ValIle: 3.788 ± 0.05
1.987ValLys: 1.987 ± 0.045
6.207ValLeu: 6.207 ± 0.091
1.69ValMet: 1.69 ± 0.042
1.929ValAsn: 1.929 ± 0.05
3.924ValPro: 3.924 ± 0.06
2.016ValGln: 2.016 ± 0.042
5.365ValArg: 5.365 ± 0.064
3.82ValSer: 3.82 ± 0.062
4.42ValThr: 4.42 ± 0.075
5.183ValVal: 5.183 ± 0.083
0.759ValTrp: 0.759 ± 0.028
1.299ValTyr: 1.299 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.521TrpAla: 1.521 ± 0.04
0.133TrpCys: 0.133 ± 0.012
0.73TrpAsp: 0.73 ± 0.026
0.571TrpGlu: 0.571 ± 0.021
0.523TrpPhe: 0.523 ± 0.023
0.935TrpGly: 0.935 ± 0.032
0.328TrpHis: 0.328 ± 0.014
0.691TrpIle: 0.691 ± 0.025
0.444TrpLys: 0.444 ± 0.02
1.679TrpLeu: 1.679 ± 0.04
0.375TrpMet: 0.375 ± 0.019
0.424TrpAsn: 0.424 ± 0.02
0.731TrpPro: 0.731 ± 0.028
0.596TrpGln: 0.596 ± 0.024
1.317TrpArg: 1.317 ± 0.029
0.766TrpSer: 0.766 ± 0.025
0.831TrpThr: 0.831 ± 0.028
0.786TrpVal: 0.786 ± 0.028
0.223TrpTrp: 0.223 ± 0.015
0.293TrpTyr: 0.293 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.739TyrAla: 2.739 ± 0.053
0.213TyrCys: 0.213 ± 0.013
1.506TyrAsp: 1.506 ± 0.034
1.031TyrGlu: 1.031 ± 0.031
0.857TyrPhe: 0.857 ± 0.026
2.166TyrGly: 2.166 ± 0.049
0.498TyrHis: 0.498 ± 0.021
0.87TyrIle: 0.87 ± 0.031
0.589TyrLys: 0.589 ± 0.021
2.039TyrLeu: 2.039 ± 0.046
0.43TyrMet: 0.43 ± 0.02
0.568TyrAsn: 0.568 ± 0.019
1.034TyrPro: 1.034 ± 0.029
0.672TyrGln: 0.672 ± 0.026
1.962TyrArg: 1.962 ± 0.04
1.196TyrSer: 1.196 ± 0.035
0.995TyrThr: 0.995 ± 0.034
1.582TyrVal: 1.582 ± 0.033
0.364TyrTrp: 0.364 ± 0.019
0.623TyrTyr: 0.623 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3846 proteins (1230456 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski