Donkey orchid symptomless virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Platypuvirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5LWR9|V5LWR9_9VIRU RNA-directed RNA polymerase OS=Donkey orchid symptomless virus OX=1400526 PE=4 SV=1
MM1 pKa = 7.45SLALCRR7 pKa = 11.84QSCHH11 pKa = 4.75SCYY14 pKa = 10.42VFQFFEE20 pKa = 4.04FQMAIINPVTSVRR33 pKa = 11.84WAQMLNNHH41 pKa = 5.45TRR43 pKa = 11.84ASPKK47 pKa = 9.67VAHH50 pKa = 6.2FQNGSDD56 pKa = 3.22CWAPLNFDD64 pKa = 3.06EE65 pKa = 6.33RR66 pKa = 11.84IYY68 pKa = 11.59VDD70 pKa = 4.13LQTGSSFQSSIPVFKK85 pKa = 9.02TTSTDD90 pKa = 3.19NVALLIFAAAYY101 pKa = 9.12DD102 pKa = 3.87RR103 pKa = 11.84RR104 pKa = 11.84ADD106 pKa = 3.93GPFHH110 pKa = 7.57LLGTDD115 pKa = 3.3NNGYY119 pKa = 10.57LYY121 pKa = 9.18TLPSGGTRR129 pKa = 11.84AIKK132 pKa = 10.63VLDD135 pKa = 3.54SFRR138 pKa = 11.84ITVRR142 pKa = 11.84PLEE145 pKa = 4.08QPYY148 pKa = 10.66VDD150 pKa = 3.58AKK152 pKa = 10.39LYY154 pKa = 10.37HH155 pKa = 6.34YY156 pKa = 10.45SVSDD160 pKa = 3.63NFPLSEE166 pKa = 3.93GQARR170 pKa = 11.84IRR172 pKa = 11.84EE173 pKa = 4.14LTIQVEE179 pKa = 4.77TLTSTNEE186 pKa = 3.6DD187 pKa = 3.36LTRR190 pKa = 11.84LNTTYY195 pKa = 10.97SSRR198 pKa = 11.84IDD200 pKa = 3.75SLQKK204 pKa = 11.15DD205 pKa = 3.81NDD207 pKa = 3.76RR208 pKa = 11.84VSAANSTLSDD218 pKa = 3.45EE219 pKa = 4.92KK220 pKa = 10.97SACFSKK226 pKa = 10.61LAQATDD232 pKa = 3.73VIRR235 pKa = 11.84QLRR238 pKa = 11.84EE239 pKa = 3.68TNALALGQIGEE250 pKa = 4.27LSSLNEE256 pKa = 3.9VLGSKK261 pKa = 10.56NDD263 pKa = 4.12DD264 pKa = 3.28LTEE267 pKa = 4.08AQRR270 pKa = 11.84LLEE273 pKa = 4.09TSLAVIEE280 pKa = 4.47TKK282 pKa = 10.64NRR284 pKa = 11.84EE285 pKa = 4.12LTVEE289 pKa = 4.13VAEE292 pKa = 4.79LKK294 pKa = 10.91AKK296 pKa = 10.87LEE298 pKa = 4.22ACKK301 pKa = 8.84PTSISPLPLTHH312 pKa = 7.13PALGVNHH319 pKa = 7.13LYY321 pKa = 10.31CWFRR325 pKa = 11.84HH326 pKa = 6.0AGTQLKK332 pKa = 10.02RR333 pKa = 11.84RR334 pKa = 11.84ARR336 pKa = 11.84TDD338 pKa = 3.33LPTMSNYY345 pKa = 9.42IVASRR350 pKa = 11.84IEE352 pKa = 4.25RR353 pKa = 11.84FAEE356 pKa = 3.79LTLIEE361 pKa = 4.31VALLVDD367 pKa = 3.55NTAIKK372 pKa = 9.81IASVTTTGSIISTPQVDD389 pKa = 4.53SLICLHH395 pKa = 7.1LGICFCC401 pKa = 5.81

Molecular weight: 44.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5LUG3|V5LUG3_9VIRU Movement protein OS=Donkey orchid symptomless virus OX=1400526 PE=4 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84EE3 pKa = 3.95NQPNSSITAANEE15 pKa = 3.77TRR17 pKa = 11.84SPLEE21 pKa = 4.03LEE23 pKa = 3.98KK24 pKa = 11.04HH25 pKa = 5.6RR26 pKa = 11.84VDD28 pKa = 4.8LQSSDD33 pKa = 2.58HH34 pKa = 6.71RR35 pKa = 11.84LRR37 pKa = 11.84MSCYY41 pKa = 10.09LKK43 pKa = 9.65STQPSRR49 pKa = 11.84MSTSRR54 pKa = 11.84QSSRR58 pKa = 11.84LGTGPNTTMRR68 pKa = 11.84STKK71 pKa = 9.73PGRR74 pKa = 11.84PTHH77 pKa = 5.47TTSTMRR83 pKa = 11.84PPNFSLGSGFPPFPAMRR100 pKa = 11.84PPSSTAMEE108 pKa = 4.01KK109 pKa = 9.41TRR111 pKa = 11.84PSKK114 pKa = 9.74ITSLPTSNTNSQAPAASASSSPLSCSSLATTPEE147 pKa = 3.97PRR149 pKa = 11.84TSSSTLTLLRR159 pKa = 11.84RR160 pKa = 11.84TSTDD164 pKa = 2.63SPRR167 pKa = 11.84TTLSPTSSNSTTPPPSCRR185 pKa = 11.84MLCTTTPQASSSTSSKK201 pKa = 8.28EE202 pKa = 3.88TLVFGSFTPQLLSQLKK218 pKa = 9.68SCTSTLPFTLAYY230 pKa = 8.17TPSSITTTTSSHH242 pKa = 6.19TSQSPPPPEE251 pKa = 4.35PTPKK255 pKa = 10.56ALPALTGSNTPTSPGAKK272 pKa = 8.59PKK274 pKa = 10.77CPALSSKK281 pKa = 10.49PLGPTMSSISCAGSFCHH298 pKa = 7.43KK299 pKa = 10.78SGDD302 pKa = 3.9CSNILHH308 pKa = 6.52SSSCPRR314 pKa = 11.84YY315 pKa = 7.08TAWGASIRR323 pKa = 11.84TNPSQKK329 pKa = 10.0RR330 pKa = 11.84SCKK333 pKa = 10.07CFSCMRR339 pKa = 11.84TPSKK343 pKa = 10.68KK344 pKa = 10.64LEE346 pKa = 4.21TSTSGPSSASKK357 pKa = 10.22FRR359 pKa = 11.84RR360 pKa = 11.84TPSTTMTSEE369 pKa = 3.79ISHH372 pKa = 6.36SSRR375 pKa = 11.84TTSSSPPNSVGTQMRR390 pKa = 11.84SPSPTRR396 pKa = 11.84ASSGAYY402 pKa = 8.99QRR404 pKa = 11.84TPRR407 pKa = 11.84TPYY410 pKa = 9.79GAHH413 pKa = 6.36SPHH416 pKa = 7.14SSVQTASKK424 pKa = 8.28NTSVNSSSSRR434 pKa = 11.84SITPSRR440 pKa = 11.84PNATSQTTYY449 pKa = 11.38QLLCTLGSKK458 pKa = 8.43TATQLISPATNCSSLRR474 pKa = 11.84RR475 pKa = 11.84ATAKK479 pKa = 6.47WTRR482 pKa = 11.84RR483 pKa = 11.84PSADD487 pKa = 3.1ANQMYY492 pKa = 10.31RR493 pKa = 11.84IVEE496 pKa = 3.92QTEE499 pKa = 3.86WEE501 pKa = 4.58TRR503 pKa = 11.84CLHH506 pKa = 5.79IMEE509 pKa = 4.95WSFPLQSYY517 pKa = 8.07TMACSLSCPVNATGAIRR534 pKa = 11.84NWRR537 pKa = 11.84VCSNSRR543 pKa = 11.84LSKK546 pKa = 9.21NQAVRR551 pKa = 11.84IMRR554 pKa = 11.84EE555 pKa = 3.59HH556 pKa = 6.54QIFKK560 pKa = 8.91TAHH563 pKa = 6.17PPSTPVSSQSMTHH576 pKa = 5.28IHH578 pKa = 7.58ASRR581 pKa = 11.84QPQPSTSLSASMGHH595 pKa = 5.7RR596 pKa = 11.84HH597 pKa = 6.02SPPTALVVPPTSPLISTEE615 pKa = 3.78AGLALSTDD623 pKa = 3.83GKK625 pKa = 8.94ITPASSSGNTCVMGSEE641 pKa = 4.4TKK643 pKa = 8.84PTATLTTT650 pKa = 3.93

Molecular weight: 69.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3120

202

1396

520.0

57.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.365 ± 0.585

2.244 ± 0.279

4.647 ± 1.051

5.321 ± 0.865

3.365 ± 0.45

4.423 ± 0.307

3.814 ± 0.657

4.968 ± 0.605

4.038 ± 0.166

9.135 ± 1.009

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.538 ± 0.437

4.103 ± 0.284

7.308 ± 0.991

3.91 ± 0.087

6.378 ± 0.138

9.327 ± 2.989

8.205 ± 1.744

4.872 ± 0.767

0.769 ± 0.062

3.269 ± 0.6
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski