Proteome-pI 2.0 - Proteome Isoelectric Point Database

Freshwater phage uvFW-CGR-AMD-COM-C429

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1B0XTY3|A0A1B0XTY3_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C429 OX=1838153 GN=uvFWCGRAMDCOMC429_029 PE=4 SV=1

MM1 pKa = 7.6TEE3 pKa = 4.78PGTQEE8 pKa = 3.92YY9 pKa = 10.29FLAAYY14 pKa = 10.12GEE16 pKa = 4.36IEE18 pKa = 4.33RR19 pKa = 11.84QLSAGPRR26 pKa = 11.84TGKK29 pKa = 8.25NTYY32 pKa = 10.15LYY34 pKa = 10.75AIVAKK39 pKa = 9.82QANAALTGEE48 pKa = 4.64LVDD51 pKa = 4.86ATDD54 pKa = 4.05EE55 pKa = 4.16DD56 pKa = 5.91AIMAIPIASDD66 pKa = 3.85DD67 pKa = 4.48NISHH71 pKa = 6.98LLEE74 pKa = 4.45SEE76 pKa = 4.22FLSVVAFTTPGLVATAIRR94 pKa = 11.84FGAYY98 pKa = 9.8AGLAGSTNVEE108 pKa = 3.16IMLFNNTSGYY118 pKa = 8.25MYY120 pKa = 10.51AYY122 pKa = 10.45VRR124 pKa = 11.84TLSTGQVTSSVIDD137 pKa = 3.42ISSDD141 pKa = 3.29EE142 pKa = 4.44FVTNTPGRR150 pKa = 11.84LLAAFSDD157 pKa = 4.22MPYY160 pKa = 11.32HH161 pKa = 6.01MIEE164 pKa = 4.25NNFEE168 pKa = 4.22EE169 pKa = 4.75YY170 pKa = 10.49SQLRR174 pKa = 11.84DD175 pKa = 3.36QVMSTFSEE183 pKa = 5.6FYY185 pKa = 10.51AAEE188 pKa = 3.98

Molecular weight: 20.49 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1B0XU07|A0A1B0XU07_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C429 OX=1838153 GN=uvFWCGRAMDCOMC429_031 PE=4 SV=1

MM1 pKa = 7.51PMVGKK6 pKa = 10.64KK7 pKa = 9.48EE8 pKa = 3.7FAYY11 pKa = 9.05TAKK14 pKa = 10.58GKK16 pKa = 10.04AAAKK20 pKa = 10.28KK21 pKa = 8.62EE22 pKa = 4.05AKK24 pKa = 8.86KK25 pKa = 9.14TGKK28 pKa = 9.43PMKK31 pKa = 9.7KK32 pKa = 9.93AKK34 pKa = 10.43NYY36 pKa = 10.19

Molecular weight: 3.96 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
40	0	40	9006	36	1484	225.2	24.56

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.159 ± 0.508	0.733 ± 0.16	5.541 ± 0.248	6.04 ± 0.322	2.698 ± 0.157	7.395 ± 0.419	1.754 ± 0.247	4.997 ± 0.254	6.129 ± 0.479	7.639 ± 0.519

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.554 ± 0.232	4.43 ± 0.309	4.697 ± 0.419	3.675 ± 0.219	5.074 ± 0.364	7.106 ± 0.369	7.395 ± 0.688	6.396 ± 0.241	1.388 ± 0.193	3.198 ± 0.277

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski