Moraxella phage Mcat11
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PHW1|A0A0R6PHW1_9CAUD Tail protein OS=Moraxella phage Mcat11 OX=1647526 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 YY3 pKa = 9.69 YY4 pKa = 11.02 YY5 pKa = 10.6 NEE7 pKa = 4.01 STDD10 pKa = 3.14 QYY12 pKa = 11.42 AKK14 pKa = 11.0 VLGEE18 pKa = 4.27 DD19 pKa = 3.07 AHH21 pKa = 7.38 SNLVTVIIDD30 pKa = 3.59 GKK32 pKa = 9.16 KK33 pKa = 9.8 QVMDD37 pKa = 3.16 WDD39 pKa = 4.05 EE40 pKa = 4.45 FVSQFNQLVDD50 pKa = 3.75 VYY52 pKa = 9.59 EE53 pKa = 4.72 DD54 pKa = 3.33 KK55 pKa = 11.12 SAEE58 pKa = 3.79 ISII61 pKa = 4.42
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.134
IPC2_protein 4.215
IPC_protein 4.101
Toseland 3.91
ProMoST 4.253
Dawson 4.075
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.935
Grimsley 3.821
Solomon 4.062
Lehninger 4.024
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.024
Sillero 4.215
Patrickios 3.121
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.121
Protein with the highest isoelectric point:
>tr|A0A0R6PHV3|A0A0R6PHV3_9CAUD HTH cro/C1-type domain-containing protein OS=Moraxella phage Mcat11 OX=1647526 PE=4 SV=1
MM1 pKa = 7.45 NEE3 pKa = 3.37 YY4 pKa = 10.53 RR5 pKa = 11.84 EE6 pKa = 4.1 WGKK9 pKa = 8.75 WVRR12 pKa = 11.84 DD13 pKa = 3.64 NPDD16 pKa = 3.4 CLKK19 pKa = 10.22 TKK21 pKa = 8.2 STWLMVEE28 pKa = 4.49 RR29 pKa = 11.84 SKK31 pKa = 11.46 LPANRR36 pKa = 11.84 RR37 pKa = 11.84 VYY39 pKa = 11.45 VMTDD43 pKa = 3.02 DD44 pKa = 5.27 RR45 pKa = 11.84 GLQIDD50 pKa = 3.81 RR51 pKa = 11.84 GVSEE55 pKa = 4.31 LARR58 pKa = 11.84 YY59 pKa = 9.64 CSVSANVFMMFYY71 pKa = 9.6 VWGWSHH77 pKa = 6.09 EE78 pKa = 4.39 KK79 pKa = 10.24 IAVRR83 pKa = 11.84 YY84 pKa = 9.1 LSAIGYY90 pKa = 8.4 RR91 pKa = 11.84 GKK93 pKa = 10.63 SRR95 pKa = 11.84 ASRR98 pKa = 11.84 HH99 pKa = 5.37 DD100 pKa = 3.4 VRR102 pKa = 11.84 SALIKK107 pKa = 10.73 AEE109 pKa = 4.06 SFLAGLLHH117 pKa = 6.76 KK118 pKa = 10.43 LYY120 pKa = 11.03 SFTT123 pKa = 4.73
Molecular weight: 14.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.193
IPC2_protein 9.355
IPC_protein 9.516
Toseland 9.838
ProMoST 9.648
Dawson 10.116
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.35
Grimsley 10.204
Solomon 10.145
Lehninger 10.101
Nozaki 9.882
DTASelect 9.823
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.028
Patrickios 7.585
IPC_peptide 10.145
IPC2_peptide 8.682
IPC2.peptide.svr19 8.305
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33
0
33
7570
40
1174
229.4
25.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.062 ± 0.746
0.832 ± 0.181
6.697 ± 0.329
5.733 ± 0.356
3.752 ± 0.178
6.935 ± 0.34
2.312 ± 0.32
6.288 ± 0.269
7.16 ± 0.34
8.851 ± 0.371
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.814 ± 0.206
4.782 ± 0.243
3.065 ± 0.377
4.505 ± 0.253
4.808 ± 0.321
5.839 ± 0.407
5.839 ± 0.378
6.05 ± 0.258
1.334 ± 0.186
3.342 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here