Euphorbia yellow mosaic virus - Goias [Brazil:GO:Luziania 8675:2009]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Euphorbia yellow mosaic virus

Average proteome isoelectric point is 8.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F5CPP0|F5CPP0_9GEMI Replication-associated protein OS=Euphorbia yellow mosaic virus - Goias [Brazil:GO:Luziania 8675:2009] OX=1016875 PE=3 SV=1
MM1 pKa = 7.5LNSSSSTPPSIKK13 pKa = 9.72AQHH16 pKa = 6.18RR17 pKa = 11.84AAKK20 pKa = 9.69RR21 pKa = 11.84RR22 pKa = 11.84AIRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84IDD30 pKa = 3.8LNCGCSIFLHH40 pKa = 6.68IDD42 pKa = 3.17CADD45 pKa = 3.34YY46 pKa = 10.92GFKK49 pKa = 10.49HH50 pKa = 6.48RR51 pKa = 11.84GVHH54 pKa = 5.56HH55 pKa = 6.31CVSGRR60 pKa = 11.84EE61 pKa = 3.74FRR63 pKa = 11.84FYY65 pKa = 11.1LGGSKK70 pKa = 10.43SPLFQDD76 pKa = 3.59NRR78 pKa = 11.84GGGPDD83 pKa = 3.01IHH85 pKa = 6.14VTPDD89 pKa = 3.04IPHH92 pKa = 7.64PDD94 pKa = 3.66PVQLQPQEE102 pKa = 4.46GTEE105 pKa = 4.27STQSFPEE112 pKa = 4.62LPSLDD117 pKa = 5.53DD118 pKa = 3.76IDD120 pKa = 4.94SSFWDD125 pKa = 4.24DD126 pKa = 3.84LFKK129 pKa = 11.26

Molecular weight:
14.52 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F5CPP1|F5CPP1_9GEMI AC4 OS=Euphorbia yellow mosaic virus - Goias [Brazil:GO:Luziania 8675:2009] OX=1016875 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.15FCSCFGTCHH11 pKa = 6.43GLSSSRR17 pKa = 11.84RR18 pKa = 11.84TSEE21 pKa = 3.95SLEE24 pKa = 3.96SSTPMGSPISTVLFNSPASPTSRR47 pKa = 11.84MLGFSTSLTPDD58 pKa = 3.74GLPNFTQMRR67 pKa = 11.84NRR69 pKa = 11.84PRR71 pKa = 11.84TPTPSKK77 pKa = 8.38ITSPKK82 pKa = 9.87RR83 pKa = 11.84VIIVNPANIRR93 pKa = 11.84CLGVPSPIKK102 pKa = 8.73MTSTTTLSMQQLQEE116 pKa = 4.16RR117 pKa = 11.84PSTT120 pKa = 3.67

Molecular weight:
12.98 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1014

120

384

202.8

23.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.325 ± 0.959

1.874 ± 0.382

5.325 ± 1.031

3.649 ± 0.286

4.832 ± 0.579

5.72 ± 0.491

3.254 ± 0.505

5.621 ± 0.527

5.819 ± 1.082

7.101 ± 1.122

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.777

5.917 ± 0.715

6.607 ± 0.966

3.057 ± 0.309

7.199 ± 0.598

8.58 ± 1.468

5.72 ± 1.228

6.41 ± 1.134

1.578 ± 0.334

4.142 ± 0.929

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski