Euphorbia yellow mosaic virus - Goias [Brazil:GO:Luziania 8675:2009]
Average proteome isoelectric point is 8.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F5CPP0|F5CPP0_9GEMI Replication-associated protein OS=Euphorbia yellow mosaic virus - Goias [Brazil:GO:Luziania 8675:2009] OX=1016875 PE=3 SV=1
MM1 pKa = 7.5 LNSSSSTPPSIKK13 pKa = 9.72 AQHH16 pKa = 6.18 RR17 pKa = 11.84 AAKK20 pKa = 9.69 RR21 pKa = 11.84 RR22 pKa = 11.84 AIRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 IDD30 pKa = 3.8 LNCGCSIFLHH40 pKa = 6.68 IDD42 pKa = 3.17 CADD45 pKa = 3.34 YY46 pKa = 10.92 GFKK49 pKa = 10.49 HH50 pKa = 6.48 RR51 pKa = 11.84 GVHH54 pKa = 5.56 HH55 pKa = 6.31 CVSGRR60 pKa = 11.84 EE61 pKa = 3.74 FRR63 pKa = 11.84 FYY65 pKa = 11.1 LGGSKK70 pKa = 10.43 SPLFQDD76 pKa = 3.59 NRR78 pKa = 11.84 GGGPDD83 pKa = 3.01 IHH85 pKa = 6.14 VTPDD89 pKa = 3.04 IPHH92 pKa = 7.64 PDD94 pKa = 3.66 PVQLQPQEE102 pKa = 4.46 GTEE105 pKa = 4.27 STQSFPEE112 pKa = 4.62 LPSLDD117 pKa = 5.53 DD118 pKa = 3.76 IDD120 pKa = 4.94 SSFWDD125 pKa = 4.24 DD126 pKa = 3.84 LFKK129 pKa = 11.26
Molecular weight: 14.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.614
IPC2_protein 6.478
IPC_protein 6.737
Toseland 6.795
ProMoST 7.307
Dawson 7.22
Bjellqvist 7.044
Wikipedia 7.19
Rodwell 7.205
Grimsley 6.898
Solomon 7.278
Lehninger 7.293
Nozaki 7.424
DTASelect 7.512
Thurlkill 7.541
EMBOSS 7.585
Sillero 7.629
Patrickios 4.469
IPC_peptide 7.293
IPC2_peptide 7.307
IPC2.peptide.svr19 7.1
Protein with the highest isoelectric point:
>tr|F5CPP1|F5CPP1_9GEMI AC4 OS=Euphorbia yellow mosaic virus - Goias [Brazil:GO:Luziania 8675:2009] OX=1016875 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.15 FCSCFGTCHH11 pKa = 6.43 GLSSSRR17 pKa = 11.84 RR18 pKa = 11.84 TSEE21 pKa = 3.95 SLEE24 pKa = 3.96 SSTPMGSPISTVLFNSPASPTSRR47 pKa = 11.84 MLGFSTSLTPDD58 pKa = 3.74 GLPNFTQMRR67 pKa = 11.84 NRR69 pKa = 11.84 PRR71 pKa = 11.84 TPTPSKK77 pKa = 8.38 ITSPKK82 pKa = 9.87 RR83 pKa = 11.84 VIIVNPANIRR93 pKa = 11.84 CLGVPSPIKK102 pKa = 8.73 MTSTTTLSMQQLQEE116 pKa = 4.16 RR117 pKa = 11.84 PSTT120 pKa = 3.67
Molecular weight: 12.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.516
IPC_protein 10.321
Toseland 11.023
ProMoST 10.979
Dawson 11.038
Bjellqvist 10.833
Wikipedia 11.316
Rodwell 11.155
Grimsley 11.067
Solomon 11.316
Lehninger 11.272
Nozaki 11.038
DTASelect 10.818
Thurlkill 10.994
EMBOSS 11.447
Sillero 11.008
Patrickios 10.95
IPC_peptide 11.33
IPC2_peptide 10.292
IPC2.peptide.svr19 9.097
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1014
120
384
202.8
23.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.325 ± 0.959
1.874 ± 0.382
5.325 ± 1.031
3.649 ± 0.286
4.832 ± 0.579
5.72 ± 0.491
3.254 ± 0.505
5.621 ± 0.527
5.819 ± 1.082
7.101 ± 1.122
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.268 ± 0.777
5.917 ± 0.715
6.607 ± 0.966
3.057 ± 0.309
7.199 ± 0.598
8.58 ± 1.468
5.72 ± 1.228
6.41 ± 1.134
1.578 ± 0.334
4.142 ± 0.929
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here