Heterobasidion partitivirus 8
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7U6Q4|K7U6Q4_9VIRU Capsid protein OS=Heterobasidion partitivirus 8 OX=1249677 PE=4 SV=1
MM1 pKa = 7.6 ASSATRR7 pKa = 11.84 IQTASRR13 pKa = 11.84 VSPVEE18 pKa = 3.43 IRR20 pKa = 11.84 TPPALAAIAKK30 pKa = 9.41 KK31 pKa = 9.64 FAPVVLQDD39 pKa = 4.88 DD40 pKa = 4.44 DD41 pKa = 4.61 TPVPPSNADD50 pKa = 2.98 YY51 pKa = 10.32 FGRR54 pKa = 11.84 MNLPTNVEE62 pKa = 4.1 LDD64 pKa = 3.04 SGNYY68 pKa = 9.78 HH69 pKa = 6.72 NIDD72 pKa = 3.33 VQFDD76 pKa = 3.36 TRR78 pKa = 11.84 FILNYY83 pKa = 10.02 IMYY86 pKa = 9.24 HH87 pKa = 4.51 VTALAPEE94 pKa = 4.72 LNFKK98 pKa = 9.16 GHH100 pKa = 7.38 PYY102 pKa = 10.3 FSPLSYY108 pKa = 9.8 IGYY111 pKa = 9.38 CLHH114 pKa = 7.17 LLYY117 pKa = 11.04 ALMLACDD124 pKa = 3.41 VTFRR128 pKa = 11.84 SDD130 pKa = 3.79 KK131 pKa = 9.68 SWHH134 pKa = 6.24 AARR137 pKa = 11.84 FMTDD141 pKa = 2.93 QEE143 pKa = 4.42 RR144 pKa = 11.84 KK145 pKa = 9.99 DD146 pKa = 3.61 LYY148 pKa = 10.82 EE149 pKa = 4.03 VLLNCHH155 pKa = 6.19 MPTFLSDD162 pKa = 4.29 LFLEE166 pKa = 4.76 LSPVYY171 pKa = 10.33 DD172 pKa = 3.59 PRR174 pKa = 11.84 RR175 pKa = 11.84 NNLLFVPSLAGFSFEE190 pKa = 4.7 HH191 pKa = 6.97 DD192 pKa = 4.04 FGRR195 pKa = 11.84 TIPPSLFYY203 pKa = 10.53 AAHH206 pKa = 6.59 HH207 pKa = 6.01 MLASTRR213 pKa = 11.84 TNKK216 pKa = 10.37 DD217 pKa = 3.26 PNDD220 pKa = 3.98 VIDD223 pKa = 5.15 DD224 pKa = 4.02 CMSLNVITAGATTYY238 pKa = 10.58 KK239 pKa = 10.42 VSNFLGTWYY248 pKa = 10.63 ASGHH252 pKa = 5.92 HH253 pKa = 6.98 DD254 pKa = 2.79 NWLNRR259 pKa = 11.84 DD260 pKa = 3.55 FLAFFNPLVGRR271 pKa = 11.84 FLTQRR276 pKa = 11.84 PTFARR281 pKa = 11.84 IHH283 pKa = 6.57 TDD285 pKa = 3.08 TEE287 pKa = 4.02 TLAIDD292 pKa = 3.92 GTGNPYY298 pKa = 8.8 TAYY301 pKa = 11.09 LLASDD306 pKa = 4.73 EE307 pKa = 4.42 NTSVMTTLLTAMSTFILANDD327 pKa = 4.05 PKK329 pKa = 10.63 APKK332 pKa = 9.79 LGSVLATLSGTLLLSYY348 pKa = 10.33 SIEE351 pKa = 4.41 PPTLPTWTAATYY363 pKa = 7.69 TQEE366 pKa = 3.98 TDD368 pKa = 3.24 PADD371 pKa = 3.65 VNDD374 pKa = 3.46 ATFARR379 pKa = 11.84 EE380 pKa = 4.1 HH381 pKa = 6.42 NFLADD386 pKa = 3.58 EE387 pKa = 4.2 PVYY390 pKa = 10.8 TNDD393 pKa = 4.61 GEE395 pKa = 4.6 YY396 pKa = 10.23 PDD398 pKa = 5.15 DD399 pKa = 3.75 TTNGNMQLYY408 pKa = 9.22 HH409 pKa = 6.56 FAHH412 pKa = 7.38 GAPAHH417 pKa = 5.33 TAARR421 pKa = 11.84 TPYY424 pKa = 10.21 KK425 pKa = 10.43 HH426 pKa = 6.54 IVFNARR432 pKa = 11.84 YY433 pKa = 8.45 HH434 pKa = 5.16 LTPYY438 pKa = 10.77 VMYY441 pKa = 8.66 FQPYY445 pKa = 8.83 DD446 pKa = 3.64 VSPSSLGLTIAAGIKK461 pKa = 9.46 IEE463 pKa = 4.62 HH464 pKa = 6.84 GDD466 pKa = 3.27 IAGFAVNIEE475 pKa = 4.18 QPEE478 pKa = 4.23 SSLDD482 pKa = 3.7 DD483 pKa = 3.68 NNAQILQSAIRR494 pKa = 11.84 LNKK497 pKa = 9.77 IIRR500 pKa = 11.84 VNANTTAGNNASRR513 pKa = 11.84 VVQRR517 pKa = 11.84 DD518 pKa = 3.04 HH519 pKa = 7.9 LDD521 pKa = 3.32 RR522 pKa = 11.84 TKK524 pKa = 10.85 QGIMTIFRR532 pKa = 11.84 SIAKK536 pKa = 9.96 SVFPYY541 pKa = 10.73 LDD543 pKa = 3.35 GSAIHH548 pKa = 5.65 YY549 pKa = 7.7 TPATLPRR556 pKa = 11.84 NLGLTEE562 pKa = 4.04 EE563 pKa = 4.48 AGHH566 pKa = 6.55 YY567 pKa = 10.34 SLQQGFNVKK576 pKa = 10.28 AGTEE580 pKa = 4.36 GKK582 pKa = 8.46 FTAPDD587 pKa = 3.23 HH588 pKa = 7.26 SIYY591 pKa = 10.55 LWSSYY596 pKa = 10.72 RR597 pKa = 11.84 YY598 pKa = 8.02 VHH600 pKa = 6.49 KK601 pKa = 10.6 KK602 pKa = 10.09 KK603 pKa = 10.65 NPAPADD609 pKa = 3.15 ISMLATLRR617 pKa = 11.84 TFYY620 pKa = 10.97 GSNVTLSRR628 pKa = 11.84 SKK630 pKa = 11.16 NPVLMIPHH638 pKa = 7.3
Molecular weight: 71.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.779
IPC2_protein 5.728
IPC_protein 5.817
Toseland 6.376
ProMoST 6.16
Dawson 6.135
Bjellqvist 6.122
Wikipedia 6.173
Rodwell 6.135
Grimsley 6.605
Solomon 6.135
Lehninger 6.135
Nozaki 6.427
DTASelect 6.62
Thurlkill 6.649
EMBOSS 6.605
Sillero 6.517
Patrickios 3.668
IPC_peptide 6.16
IPC2_peptide 6.532
IPC2.peptide.svr19 6.447
Protein with the highest isoelectric point:
>tr|K7U6Q4|K7U6Q4_9VIRU Capsid protein OS=Heterobasidion partitivirus 8 OX=1249677 PE=4 SV=1
MM1 pKa = 7.39 LSKK4 pKa = 10.76 VRR6 pKa = 11.84 DD7 pKa = 3.98 FFHH10 pKa = 7.12 EE11 pKa = 4.08 KK12 pKa = 10.4 LSRR15 pKa = 11.84 LLLQHH20 pKa = 7.19 SIFQSNDD27 pKa = 2.54 KK28 pKa = 11.21 DD29 pKa = 3.79 EE30 pKa = 4.45 EE31 pKa = 4.22 QTLLEE36 pKa = 4.36 NQSSDD41 pKa = 2.88 VKK43 pKa = 10.59 RR44 pKa = 11.84 IYY46 pKa = 11.07 NSIHH50 pKa = 6.42 YY51 pKa = 9.74 SFQTDD56 pKa = 3.48 DD57 pKa = 3.26 TQAAYY62 pKa = 10.03 EE63 pKa = 4.37 DD64 pKa = 4.24 QYY66 pKa = 11.56 QHH68 pKa = 6.37 IKK70 pKa = 10.44 HH71 pKa = 5.68 VLEE74 pKa = 5.12 DD75 pKa = 3.85 KK76 pKa = 10.95 DD77 pKa = 3.76 RR78 pKa = 11.84 LSNFPAEE85 pKa = 4.76 FYY87 pKa = 10.96 LPYY90 pKa = 10.06 DD91 pKa = 3.62 TTTTPDD97 pKa = 3.0 NRR99 pKa = 11.84 VPPSGIDD106 pKa = 3.24 QLPYY110 pKa = 9.75 VYY112 pKa = 10.66 KK113 pKa = 9.56 RR114 pKa = 11.84 TNVVTATDD122 pKa = 3.91 EE123 pKa = 4.35 VPEE126 pKa = 4.06 TGYY129 pKa = 9.95 PIQNRR134 pKa = 11.84 LLRR137 pKa = 11.84 LIRR140 pKa = 11.84 SRR142 pKa = 11.84 YY143 pKa = 7.28 PQYY146 pKa = 10.62 LPHH149 pKa = 6.38 VRR151 pKa = 11.84 TFTRR155 pKa = 11.84 PLGTTDD161 pKa = 2.98 ATVSDD166 pKa = 4.67 FFKK169 pKa = 10.07 PQHH172 pKa = 6.49 PSRR175 pKa = 11.84 LVDD178 pKa = 3.71 PSRR181 pKa = 11.84 ISHH184 pKa = 5.13 VMKK187 pKa = 10.62 HH188 pKa = 5.76 VMNKK192 pKa = 8.36 MAITPYY198 pKa = 10.69 LPIHH202 pKa = 6.26 FVDD205 pKa = 3.47 TQYY208 pKa = 11.77 DD209 pKa = 3.79 KK210 pKa = 11.46 RR211 pKa = 11.84 PLSTGTGYY219 pKa = 10.91 YY220 pKa = 10.02 NRR222 pKa = 11.84 RR223 pKa = 11.84 SHH225 pKa = 6.34 EE226 pKa = 4.28 ANIHH230 pKa = 5.7 ALYY233 pKa = 10.35 SHH235 pKa = 7.03 PKK237 pKa = 8.41 EE238 pKa = 4.3 YY239 pKa = 10.69 EE240 pKa = 3.71 NKK242 pKa = 8.33 RR243 pKa = 11.84 TSKK246 pKa = 10.61 GYY248 pKa = 10.13 YY249 pKa = 8.92 INAFLEE255 pKa = 4.47 SARR258 pKa = 11.84 SLVHH262 pKa = 6.62 WIKK265 pKa = 11.0 SYY267 pKa = 11.51 GNPFRR272 pKa = 11.84 SKK274 pKa = 10.12 PADD277 pKa = 3.33 PRR279 pKa = 11.84 EE280 pKa = 4.08 SLKK283 pKa = 10.94 KK284 pKa = 10.52 FFLQRR289 pKa = 11.84 PTMLFTRR296 pKa = 11.84 NHH298 pKa = 5.57 ISKK301 pKa = 10.34 ILGALKK307 pKa = 9.86 QRR309 pKa = 11.84 PVYY312 pKa = 10.64 AVDD315 pKa = 5.16 DD316 pKa = 4.18 LFLTLEE322 pKa = 4.22 SMVTFPAHH330 pKa = 5.7 TIARR334 pKa = 11.84 KK335 pKa = 9.53 IEE337 pKa = 3.89 CCIMYY342 pKa = 9.9 GYY344 pKa = 8.32 EE345 pKa = 4.2 TIRR348 pKa = 11.84 GSNVQLDD355 pKa = 4.04 RR356 pKa = 11.84 LAQRR360 pKa = 11.84 YY361 pKa = 8.96 NSFFTIDD368 pKa = 2.88 WSGFDD373 pKa = 4.51 QRR375 pKa = 11.84 LPWVIVLLFFTEE387 pKa = 4.43 FLPRR391 pKa = 11.84 LLVINHH397 pKa = 6.91 GYY399 pKa = 10.56 APTYY403 pKa = 9.87 DD404 pKa = 3.63 FPSYY408 pKa = 10.67 PDD410 pKa = 3.43 LTTEE414 pKa = 3.76 MMYY417 pKa = 10.55 QRR419 pKa = 11.84 LSNILSFLATWYY431 pKa = 10.65 FNMVFITADD440 pKa = 2.7 GFAYY444 pKa = 9.87 VRR446 pKa = 11.84 RR447 pKa = 11.84 FAGVPSGLLNTQFLDD462 pKa = 3.52 SFGNLFLIIDD472 pKa = 3.84 ALIEE476 pKa = 4.05 FGAQDD481 pKa = 3.8 EE482 pKa = 5.07 EE483 pKa = 4.5 IDD485 pKa = 4.79 SILLLIMGDD494 pKa = 3.84 DD495 pKa = 3.68 NSGFTIWSIARR506 pKa = 11.84 LEE508 pKa = 3.93 QFITFLEE515 pKa = 4.99 SYY517 pKa = 10.76 ALTRR521 pKa = 11.84 YY522 pKa = 9.48 GMVMSKK528 pKa = 9.09 TKK530 pKa = 10.84 SLVTVLRR537 pKa = 11.84 HH538 pKa = 6.49 KK539 pKa = 10.52 IQTLSYY545 pKa = 8.4 TCNFGRR551 pKa = 11.84 PLRR554 pKa = 11.84 PIPEE558 pKa = 4.38 LVAHH562 pKa = 6.37 LVFPEE567 pKa = 4.63 RR568 pKa = 11.84 EE569 pKa = 4.1 FKK571 pKa = 10.1 PQFMSARR578 pKa = 11.84 AVGMAWASCAQDD590 pKa = 3.22 KK591 pKa = 9.26 TFHH594 pKa = 6.69 DD595 pKa = 4.71 FCRR598 pKa = 11.84 DD599 pKa = 2.86 VFYY602 pKa = 10.52 EE603 pKa = 3.96 YY604 pKa = 10.92 LEE606 pKa = 4.25 EE607 pKa = 4.66 SVPVDD612 pKa = 3.3 NTNIAWIQSHH622 pKa = 6.37 LPGYY626 pKa = 10.34 LRR628 pKa = 11.84 VDD630 pKa = 3.52 PEE632 pKa = 4.16 VTKK635 pKa = 10.55 MIDD638 pKa = 3.39 LNVFPSFLHH647 pKa = 6.08 VSQKK651 pKa = 10.14 LSRR654 pKa = 11.84 WQGPLSYY661 pKa = 10.28 QPKK664 pKa = 8.69 WDD666 pKa = 3.58 LAHH669 pKa = 7.45 FINQPDD675 pKa = 4.46 VIPDD679 pKa = 3.44 DD680 pKa = 4.7 SITMFEE686 pKa = 4.06 YY687 pKa = 9.82 MQEE690 pKa = 4.03 HH691 pKa = 6.57 SLSLDD696 pKa = 3.06 IQFDD700 pKa = 3.97 LFSAA704 pKa = 4.31
Molecular weight: 82.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.127
IPC2_protein 6.059
IPC_protein 6.173
Toseland 6.649
ProMoST 6.576
Dawson 6.504
Bjellqvist 6.478
Wikipedia 6.532
Rodwell 6.491
Grimsley 6.854
Solomon 6.504
Lehninger 6.491
Nozaki 6.781
DTASelect 6.971
Thurlkill 7.0
EMBOSS 6.971
Sillero 6.883
Patrickios 3.986
IPC_peptide 6.517
IPC2_peptide 6.81
IPC2.peptide.svr19 6.777
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1342
638
704
671.0
76.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.079 ± 1.707
0.671 ± 0.03
6.334 ± 0.044
3.949 ± 0.56
6.11 ± 0.645
4.024 ± 0.467
3.651 ± 0.184
5.44 ± 0.399
3.651 ± 0.355
9.985 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.459 ± 0.074
4.918 ± 0.822
6.408 ± 0.228
3.502 ± 0.792
5.365 ± 0.564
7.228 ± 0.228
7.675 ± 0.866
5.291 ± 0.189
1.043 ± 0.178
5.216 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here