Streptococcus satellite phage Javan740
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZZR0|A0A4D5ZZR0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan740 OX=2558834 GN=JavanS740_0024 PE=4 SV=1
MM1 pKa = 7.87 EE2 pKa = 4.51 EE3 pKa = 3.56 TTYY6 pKa = 11.03 DD7 pKa = 3.82 IIAKK11 pKa = 9.74 SLDD14 pKa = 4.0 RR15 pKa = 11.84 ISMEE19 pKa = 3.74 LHH21 pKa = 5.91 QADD24 pKa = 4.33 EE25 pKa = 4.59 NNDD28 pKa = 3.14 FLEE31 pKa = 4.3 YY32 pKa = 10.95 GFYY35 pKa = 10.31 QDD37 pKa = 3.87 NN38 pKa = 3.58
Molecular weight: 4.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A4D5ZUV7|A0A4D5ZUV7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan740 OX=2558834 GN=JavanS740_0007 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.33 SIEE5 pKa = 4.04 RR6 pKa = 11.84 QLFKK10 pKa = 10.96 LVLSHH15 pKa = 7.03 AFNLASLSVRR25 pKa = 11.84 LVARR29 pKa = 11.84 KK30 pKa = 9.53 KK31 pKa = 10.54 LYY33 pKa = 10.37 KK34 pKa = 10.0 KK35 pKa = 8.72 QLKK38 pKa = 8.08 KK39 pKa = 8.76 THH41 pKa = 5.44 AQKK44 pKa = 10.77 FGDD47 pKa = 4.2 RR48 pKa = 11.84 EE49 pKa = 3.91 RR50 pKa = 11.84 EE51 pKa = 3.84 ACDD54 pKa = 3.06 RR55 pKa = 11.84 KK56 pKa = 10.33 NCIRR60 pKa = 11.84 NNHH63 pKa = 5.81 SKK65 pKa = 10.57 SPHH68 pKa = 6.08 SNFGQGEE75 pKa = 4.34 RR76 pKa = 11.84 EE77 pKa = 3.74 DD78 pKa = 4.01 SNYY81 pKa = 10.17 KK82 pKa = 10.13 KK83 pKa = 10.38 KK84 pKa = 10.63 ALRR87 pKa = 11.84 SHH89 pKa = 5.94 QGNYY93 pKa = 6.12 TTT95 pKa = 5.45
Molecular weight: 11.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.239
IPC2_protein 9.663
IPC_protein 9.794
Toseland 10.687
ProMoST 10.189
Dawson 10.76
Bjellqvist 10.365
Wikipedia 10.877
Rodwell 11.316
Grimsley 10.789
Solomon 10.804
Lehninger 10.789
Nozaki 10.657
DTASelect 10.365
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.672
Patrickios 11.052
IPC_peptide 10.818
IPC2_peptide 9.019
IPC2.peptide.svr19 8.659
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31
0
31
4183
38
484
134.9
15.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.498 ± 0.33
0.645 ± 0.109
5.905 ± 0.368
9.228 ± 0.647
3.801 ± 0.325
4.662 ± 0.294
1.41 ± 0.212
6.718 ± 0.439
9.419 ± 0.574
10.232 ± 0.391
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.725 ± 0.239
5.451 ± 0.354
2.869 ± 0.395
4.208 ± 0.263
5.451 ± 0.287
5.379 ± 0.344
5.594 ± 0.456
5.666 ± 0.31
0.861 ± 0.195
4.279 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here