Microbacterium phage Hubbs

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XKM2|A0A6G6XKM2_9CAUD Exonuclease OS=Microbacterium phage Hubbs OX=2704038 GN=72 PE=4 SV=1
MM1 pKa = 7.58AVTHH5 pKa = 5.54NTIEE9 pKa = 4.34GAPGIPMIIEE19 pKa = 4.34SPDD22 pKa = 3.5TFPFAPGEE30 pKa = 4.1RR31 pKa = 11.84DD32 pKa = 4.07SEE34 pKa = 4.19WHH36 pKa = 6.55DD37 pKa = 3.53TTDD40 pKa = 3.67AEE42 pKa = 4.6YY43 pKa = 10.79AVYY46 pKa = 9.82AAYY49 pKa = 9.29WDD51 pKa = 4.1SQDD54 pKa = 4.2GAFDD58 pKa = 3.94PAPEE62 pKa = 3.98GWEE65 pKa = 4.0DD66 pKa = 3.93YY67 pKa = 10.84EE68 pKa = 4.59LVNAAQHH75 pKa = 5.98AASAHH80 pKa = 6.22LDD82 pKa = 3.64GEE84 pKa = 4.57LL85 pKa = 3.98

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XKL0|A0A6G6XKL0_9CAUD Uncharacterized protein OS=Microbacterium phage Hubbs OX=2704038 GN=9 PE=4 SV=1
MM1 pKa = 7.56SVIEE5 pKa = 4.32FKK7 pKa = 10.75NARR10 pKa = 11.84GQVRR14 pKa = 11.84YY15 pKa = 9.77QPALPDD21 pKa = 3.21GRR23 pKa = 11.84RR24 pKa = 11.84LYY26 pKa = 9.92NCGWFTEE33 pKa = 4.26KK34 pKa = 10.84ALAEE38 pKa = 4.66IISEE42 pKa = 4.23SWFDD46 pKa = 3.09HH47 pKa = 6.3HH48 pKa = 7.01PRR50 pKa = 11.84LYY52 pKa = 9.82RR53 pKa = 11.84SKK55 pKa = 10.68RR56 pKa = 11.84RR57 pKa = 11.84ASRR60 pKa = 11.84KK61 pKa = 8.14ASLRR65 pKa = 11.84ITEE68 pKa = 4.06EE69 pKa = 4.04YY70 pKa = 9.83IRR72 pKa = 11.84EE73 pKa = 3.98RR74 pKa = 11.84NSFKK78 pKa = 10.74EE79 pKa = 3.98VAKK82 pKa = 10.9

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

114

0

114

19077

30

1252

167.3

18.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.08 ± 0.35

0.482 ± 0.087

6.547 ± 0.213

7.533 ± 0.35

3.365 ± 0.128

8.067 ± 0.35

1.751 ± 0.136

5.321 ± 0.158

4.602 ± 0.325

7.04 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.151

3.795 ± 0.253

4.361 ± 0.247

3.575 ± 0.166

6.573 ± 0.215

6.054 ± 0.252

6.123 ± 0.228

7.239 ± 0.18

1.971 ± 0.141

3.161 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski