Bovine polyomavirus (BPyV) (Bos taurus polyomavirus 1)
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P24851|LT_POVBO Large T antigen OS=Bovine polyomavirus OX=1891754 PE=2 SV=2
MM1 pKa = 7.52 ALQWVWLCLRR11 pKa = 11.84 KK12 pKa = 9.75 SLRR15 pKa = 11.84 NNKK18 pKa = 9.66 LMMCRR23 pKa = 11.84 ILIEE27 pKa = 4.06 LLKK30 pKa = 10.66 ILLDD34 pKa = 3.75 VLDD37 pKa = 4.51 SGDD40 pKa = 3.91 YY41 pKa = 11.33 ADD43 pKa = 5.58 GPSDD47 pKa = 4.01 NPGTGIAKK55 pKa = 10.18 EE56 pKa = 4.31 EE57 pKa = 3.9 IVKK60 pKa = 10.97 CIDD63 pKa = 3.43 FLKK66 pKa = 10.31 MLEE69 pKa = 4.83 AGDD72 pKa = 5.18 DD73 pKa = 4.58 DD74 pKa = 3.72 IDD76 pKa = 4.2 KK77 pKa = 11.43 YY78 pKa = 11.41 GFPEE82 pKa = 4.06 LLAAHH87 pKa = 6.96 FANCLAVCLKK97 pKa = 10.37 AARR100 pKa = 11.84 EE101 pKa = 3.92 SDD103 pKa = 4.44 AMFCCSLEE111 pKa = 4.34 ANSSDD116 pKa = 4.02 FNN118 pKa = 4.06
Molecular weight: 13.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.458
IPC2_protein 4.52
IPC_protein 4.431
Toseland 4.24
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.317
Rodwell 4.266
Grimsley 4.151
Solomon 4.393
Lehninger 4.355
Nozaki 4.52
DTASelect 4.736
Thurlkill 4.279
EMBOSS 4.329
Sillero 4.546
Patrickios 3.592
IPC_peptide 4.393
IPC2_peptide 4.533
IPC2.peptide.svr19 4.497
Protein with the highest isoelectric point:
>sp|P24849-2|VP2-2_POVBO Isoform of P24849 Isoform VP3 of Minor capsid protein VP2 OS=Bovine polyomavirus OX=1891754 PE=3 SV=3
MM1 pKa = 7.66 EE2 pKa = 4.82 LTSEE6 pKa = 4.35 EE7 pKa = 4.34 YY8 pKa = 10.71 EE9 pKa = 4.2 EE10 pKa = 5.09 LRR12 pKa = 11.84 GLLGTPDD19 pKa = 3.43 IGNADD24 pKa = 3.95 TLKK27 pKa = 10.86 KK28 pKa = 10.77 AFLKK32 pKa = 10.23 ACKK35 pKa = 9.35 VHH37 pKa = 7.09 HH38 pKa = 7.05 PDD40 pKa = 3.73 KK41 pKa = 11.17 GGNEE45 pKa = 3.9 EE46 pKa = 3.86 AMKK49 pKa = 10.66 RR50 pKa = 11.84 LLYY53 pKa = 10.44 LYY55 pKa = 10.33 NKK57 pKa = 10.23 AKK59 pKa = 10.09 IAASATTSQVWYY71 pKa = 9.21 FLIIGYY77 pKa = 9.94 ISLKK81 pKa = 10.25 NKK83 pKa = 10.24 NIYY86 pKa = 9.27 LPKK89 pKa = 10.14 IFWLRR94 pKa = 11.84 FQNMAPHH101 pKa = 6.8 SGNSGGKK108 pKa = 9.29 NSIKK112 pKa = 10.88 ALMSKK117 pKa = 9.62 ICIVMRR123 pKa = 11.84 NN124 pKa = 3.36
Molecular weight: 14.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.084
IPC2_protein 8.975
IPC_protein 8.887
Toseland 9.648
ProMoST 9.355
Dawson 9.882
Bjellqvist 9.575
Wikipedia 10.043
Rodwell 10.335
Grimsley 9.955
Solomon 9.926
Lehninger 9.897
Nozaki 9.706
DTASelect 9.545
Thurlkill 9.736
EMBOSS 10.072
Sillero 9.809
Patrickios 7.585
IPC_peptide 9.926
IPC2_peptide 8.141
IPC2.peptide.svr19 8.047
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
1
6
1811
118
619
301.8
34.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.957 ± 1.37
2.043 ± 0.777
4.307 ± 0.809
8.227 ± 0.73
3.534 ± 0.216
6.405 ± 0.463
2.264 ± 0.43
5.135 ± 0.473
6.295 ± 1.194
10.491 ± 0.745
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.154 ± 0.496
5.135 ± 0.432
5.743 ± 0.845
3.865 ± 0.597
4.859 ± 0.815
5.632 ± 0.537
6.129 ± 0.974
5.411 ± 0.61
1.436 ± 0.218
3.976 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here