Halarsenatibacter silvermanii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halarsenatibacter

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2587 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9GXZ1|A0A1G9GXZ1_9FIRM Uncharacterized protein OS=Halarsenatibacter silvermanii OX=321763 GN=SAMN04488692_10139 PE=4 SV=1
MM1 pKa = 7.43LKK3 pKa = 10.17KK4 pKa = 10.75VSLSLALMLVIGLVFTVPAMAEE26 pKa = 3.87EE27 pKa = 5.01DD28 pKa = 3.7PLVIYY33 pKa = 7.21FFPGGPEE40 pKa = 3.94GGPFSTIVYY49 pKa = 9.99NGADD53 pKa = 3.54YY54 pKa = 11.33ADD56 pKa = 4.2EE57 pKa = 4.21VLGDD61 pKa = 3.84RR62 pKa = 11.84VDD64 pKa = 3.4VRR66 pKa = 11.84YY67 pKa = 9.65RR68 pKa = 11.84WSDD71 pKa = 3.01WSPEE75 pKa = 3.83QMVTQFQEE83 pKa = 4.19AVAANPDD90 pKa = 3.9GIAIMGHH97 pKa = 6.65PGDD100 pKa = 5.09DD101 pKa = 3.12ALEE104 pKa = 4.01PHH106 pKa = 6.35VEE108 pKa = 3.93NAKK111 pKa = 9.88EE112 pKa = 3.77QGITVTSQNTTLPALEE128 pKa = 4.23EE129 pKa = 4.41RR130 pKa = 11.84YY131 pKa = 9.86VDD133 pKa = 3.59EE134 pKa = 4.83GFGYY138 pKa = 10.66AGQDD142 pKa = 3.67LYY144 pKa = 11.67DD145 pKa = 3.66SGYY148 pKa = 8.94MLGEE152 pKa = 4.04AAVEE156 pKa = 3.86RR157 pKa = 11.84AEE159 pKa = 4.85LGEE162 pKa = 4.33GDD164 pKa = 4.52SAFIWGLKK172 pKa = 8.49AQPEE176 pKa = 4.26RR177 pKa = 11.84GQRR180 pKa = 11.84TQGAVDD186 pKa = 3.44AVEE189 pKa = 4.08EE190 pKa = 4.32AGVEE194 pKa = 3.89VDD196 pKa = 3.5YY197 pKa = 11.31MEE199 pKa = 5.03ISSEE203 pKa = 3.91VDD205 pKa = 2.72ADD207 pKa = 3.62ASAGIPVISGYY218 pKa = 10.25LAEE221 pKa = 5.52NPDD224 pKa = 3.83TDD226 pKa = 5.64LVITDD231 pKa = 4.43HH232 pKa = 6.77GALTSTMDD240 pKa = 3.58TYY242 pKa = 11.48FEE244 pKa = 4.33AAGKK248 pKa = 10.04EE249 pKa = 3.73PGEE252 pKa = 4.02IYY254 pKa = 10.75GAGFDD259 pKa = 4.89LSTATLDD266 pKa = 4.51AIQEE270 pKa = 4.57GYY272 pKa = 9.12VQLVHH277 pKa = 6.91DD278 pKa = 4.63QQPFLQGFLPIMQIYY293 pKa = 10.09ISEE296 pKa = 4.19NYY298 pKa = 8.92EE299 pKa = 3.76FSGLHH304 pKa = 6.39IDD306 pKa = 3.6TGSGLVHH313 pKa = 7.06EE314 pKa = 5.67DD315 pKa = 3.42NVDD318 pKa = 3.33VLAPLVEE325 pKa = 4.19EE326 pKa = 5.51GIRR329 pKa = 3.51

Molecular weight: 35.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9Q6X6|A0A1G9Q6X6_9FIRM Uncharacterized protein OS=Halarsenatibacter silvermanii OX=321763 GN=SAMN04488692_11569 PE=4 SV=1
MM1 pKa = 7.73IKK3 pKa = 10.32RR4 pKa = 11.84LFRR7 pKa = 11.84RR8 pKa = 11.84IFLLLIGIYY17 pKa = 9.69QRR19 pKa = 11.84FISPMLPGSCRR30 pKa = 11.84FYY32 pKa = 9.69PTCSSYY38 pKa = 10.41TRR40 pKa = 11.84QAIEE44 pKa = 4.05KK45 pKa = 8.7YY46 pKa = 10.76GPFKK50 pKa = 10.44GVWMGIKK57 pKa = 9.97RR58 pKa = 11.84ISRR61 pKa = 11.84CHH63 pKa = 6.12PFNPGGIDD71 pKa = 3.37PVEE74 pKa = 3.8

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2587

0

2587

792929

25

2051

306.5

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.28 ± 0.051

0.899 ± 0.02

6.394 ± 0.044

10.265 ± 0.075

4.067 ± 0.037

7.377 ± 0.044

1.669 ± 0.02

7.36 ± 0.043

5.666 ± 0.064

9.884 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.023

3.895 ± 0.036

3.694 ± 0.03

2.999 ± 0.024

5.461 ± 0.043

6.037 ± 0.035

4.285 ± 0.031

6.283 ± 0.041

0.801 ± 0.015

3.13 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski