Castlerea virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses; Negevirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5S5K3|A0A1W5S5K3_9VIRU ORF2 OS=Castlerea virus OX=1969351 PE=4 SV=1
MM1 pKa = 7.53NFPLTVSFLLHH12 pKa = 6.04FVALVHH18 pKa = 6.56SAVFKK23 pKa = 11.28NDD25 pKa = 3.15VLNQFPSTRR34 pKa = 11.84QKK36 pKa = 10.5NAFYY40 pKa = 10.67QKK42 pKa = 10.38LIPHH46 pKa = 7.04LEE48 pKa = 3.91PLGEE52 pKa = 4.23FEE54 pKa = 4.02IAPYY58 pKa = 10.79AFNPEE63 pKa = 4.24CLSVYY68 pKa = 8.85RR69 pKa = 11.84TNDD72 pKa = 2.55WFYY75 pKa = 10.92SQCALPSNCSEE86 pKa = 3.84PSVIKK91 pKa = 10.57LEE93 pKa = 4.09RR94 pKa = 11.84SFFGQEE100 pKa = 3.65TVICHH105 pKa = 6.61SDD107 pKa = 3.18LKK109 pKa = 10.85HH110 pKa = 5.9SRR112 pKa = 11.84KK113 pKa = 9.74DD114 pKa = 3.19VYY116 pKa = 11.35EE117 pKa = 3.96FFTPKK122 pKa = 10.22FVPKK126 pKa = 10.42NLTLRR131 pKa = 11.84DD132 pKa = 4.29PISILGNNFNLLTNLTPVSFFEE154 pKa = 3.65WFYY157 pKa = 10.86IVKK160 pKa = 10.31SADD163 pKa = 2.9GRR165 pKa = 11.84YY166 pKa = 9.89GLLPRR171 pKa = 11.84ICDD174 pKa = 3.37EE175 pKa = 3.83THH177 pKa = 6.77RR178 pKa = 11.84NSTHH182 pKa = 6.16LPKK185 pKa = 10.49NVHH188 pKa = 6.34LEE190 pKa = 4.16HH191 pKa = 7.11NGDD194 pKa = 3.81RR195 pKa = 11.84VCLIEE200 pKa = 4.08TSYY203 pKa = 8.1TTHH206 pKa = 5.95SCIPKK211 pKa = 9.67LYY213 pKa = 10.64NSIFTLLQLYY223 pKa = 8.36DD224 pKa = 4.01FPIEE228 pKa = 3.84FDD230 pKa = 3.83GVPFSQGSFSLTNNKK245 pKa = 9.52VIGADD250 pKa = 3.51SYY252 pKa = 10.26ITNSVGKK259 pKa = 8.29TPIVVDD265 pKa = 3.93KK266 pKa = 11.6VGGSYY271 pKa = 10.0MLTYY275 pKa = 10.78EE276 pKa = 4.73SFDD279 pKa = 4.02SNQPFYY285 pKa = 10.58IVRR288 pKa = 11.84NQRR291 pKa = 11.84HH292 pKa = 6.16LPTEE296 pKa = 3.64NHH298 pKa = 5.05QCFNLLSHH306 pKa = 6.63YY307 pKa = 10.51SSPLTSIFHH316 pKa = 6.87HH317 pKa = 6.67ISTFIRR323 pKa = 11.84KK324 pKa = 8.09EE325 pKa = 3.49LSYY328 pKa = 10.99LVEE331 pKa = 4.23FLKK334 pKa = 10.94QLAGEE339 pKa = 4.0IALILFKK346 pKa = 10.5IVSEE350 pKa = 3.97LFEE353 pKa = 5.08IFMSLIPYY361 pKa = 9.85NSEE364 pKa = 4.02FYY366 pKa = 10.49TSLFVMTLTYY376 pKa = 10.69FNTRR380 pKa = 11.84DD381 pKa = 3.36IPISCVVFVGVYY393 pKa = 8.4VFKK396 pKa = 10.57IYY398 pKa = 7.6MTSFII403 pKa = 4.78

Molecular weight: 46.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5S133|A0A1W5S133_9VIRU Non-structural protein 3 OS=Castlerea virus OX=1969351 PE=4 SV=1
MM1 pKa = 7.72SSRR4 pKa = 11.84PQKK7 pKa = 10.15RR8 pKa = 11.84SPGKK12 pKa = 7.74TVAAARR18 pKa = 11.84TITPKK23 pKa = 10.37KK24 pKa = 9.44RR25 pKa = 11.84AAPTAPKK32 pKa = 10.14NSRR35 pKa = 11.84RR36 pKa = 11.84PKK38 pKa = 9.82QNLGFDD44 pKa = 4.41LNAVVSDD51 pKa = 3.32ITTTFTKK58 pKa = 10.55GLNRR62 pKa = 11.84PLVLLSVVCVCALVVTHH79 pKa = 6.95SSDD82 pKa = 3.31FTSGVVGKK90 pKa = 9.2WISEE94 pKa = 4.05KK95 pKa = 10.98SSTNSAALWVHH106 pKa = 5.45QNQARR111 pKa = 11.84FLGLAIFLPAILDD124 pKa = 3.85TPDD127 pKa = 3.24SVRR130 pKa = 11.84VAVSLASVFWVMLIPAASIYY150 pKa = 10.41EE151 pKa = 3.99YY152 pKa = 10.67VIQAFALHH160 pKa = 6.5TYY162 pKa = 10.23FRR164 pKa = 11.84VQLQNSRR171 pKa = 11.84ILILLTVGVLYY182 pKa = 10.35FLGHH186 pKa = 6.0ITLSQTSPLTAPAVNGSATNN206 pKa = 3.54

Molecular weight: 22.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2978

206

2369

992.7

112.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.709 ± 1.476

2.317 ± 0.383

5.003 ± 1.031

5.641 ± 1.35

6.212 ± 1.095

4.433 ± 0.349

2.619 ± 0.377

5.809 ± 0.385

5.507 ± 0.561

9.302 ± 0.819

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.116 ± 0.513

5.44 ± 0.33

4.735 ± 0.53

3.156 ± 0.209

5.339 ± 0.756

7.992 ± 0.917

6.582 ± 0.583

7.858 ± 0.737

0.504 ± 0.263

3.727 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski