Amino acid dipepetide frequency for Gordonia phage GTE8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.714AlaAla: 11.714 ± 1.28
0.744AlaCys: 0.744 ± 0.201
6.926AlaAsp: 6.926 ± 0.653
6.508AlaGlu: 6.508 ± 0.563
3.161AlaPhe: 3.161 ± 0.42
7.577AlaGly: 7.577 ± 0.671
1.952AlaHis: 1.952 ± 0.439
5.439AlaIle: 5.439 ± 0.648
5.578AlaLys: 5.578 ± 0.634
8.135AlaLeu: 8.135 ± 1.055
2.278AlaMet: 2.278 ± 0.302
3.021AlaAsn: 3.021 ± 0.354
4.602AlaPro: 4.602 ± 0.629
3.905AlaGln: 3.905 ± 0.682
6.322AlaArg: 6.322 ± 0.57
4.695AlaSer: 4.695 ± 0.578
5.532AlaThr: 5.532 ± 0.495
7.623AlaVal: 7.623 ± 0.521
1.627AlaTrp: 1.627 ± 0.274
2.417AlaTyr: 2.417 ± 0.311
0.0AlaXaa: 0.0 ± 0.0
Cys
0.697CysAla: 0.697 ± 0.274
0.046CysCys: 0.046 ± 0.045
0.79CysAsp: 0.79 ± 0.224
0.558CysGlu: 0.558 ± 0.2
0.139CysPhe: 0.139 ± 0.075
0.837CysGly: 0.837 ± 0.211
0.372CysHis: 0.372 ± 0.149
0.465CysIle: 0.465 ± 0.135
0.139CysLys: 0.139 ± 0.071
0.604CysLeu: 0.604 ± 0.192
0.046CysMet: 0.046 ± 0.045
0.232CysAsn: 0.232 ± 0.105
0.418CysPro: 0.418 ± 0.176
0.418CysGln: 0.418 ± 0.146
0.697CysArg: 0.697 ± 0.205
0.883CysSer: 0.883 ± 0.358
0.418CysThr: 0.418 ± 0.141
1.162CysVal: 1.162 ± 0.292
0.232CysTrp: 0.232 ± 0.109
0.279CysTyr: 0.279 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
6.415AspAla: 6.415 ± 0.486
0.418AspCys: 0.418 ± 0.147
6.322AspAsp: 6.322 ± 0.683
5.717AspGlu: 5.717 ± 0.631
2.417AspPhe: 2.417 ± 0.344
6.415AspGly: 6.415 ± 0.52
1.395AspHis: 1.395 ± 0.228
3.533AspIle: 3.533 ± 0.456
2.092AspLys: 2.092 ± 0.375
5.578AspLeu: 5.578 ± 0.521
1.534AspMet: 1.534 ± 0.259
1.952AspAsn: 1.952 ± 0.277
5.485AspPro: 5.485 ± 0.527
2.231AspGln: 2.231 ± 0.332
4.137AspArg: 4.137 ± 0.473
3.068AspSer: 3.068 ± 0.411
2.975AspThr: 2.975 ± 0.394
4.323AspVal: 4.323 ± 0.463
1.487AspTrp: 1.487 ± 0.241
2.185AspTyr: 2.185 ± 0.365
0.0AspXaa: 0.0 ± 0.0
Glu
6.322GluAla: 6.322 ± 0.629
0.325GluCys: 0.325 ± 0.152
4.462GluAsp: 4.462 ± 0.567
3.858GluGlu: 3.858 ± 0.632
2.231GluPhe: 2.231 ± 0.391
4.648GluGly: 4.648 ± 0.381
1.162GluHis: 1.162 ± 0.203
3.161GluIle: 3.161 ± 0.301
2.975GluLys: 2.975 ± 0.4
5.253GluLeu: 5.253 ± 0.539
1.906GluMet: 1.906 ± 0.249
2.045GluAsn: 2.045 ± 0.323
2.696GluPro: 2.696 ± 0.416
2.231GluGln: 2.231 ± 0.323
5.392GluArg: 5.392 ± 0.72
3.626GluSer: 3.626 ± 0.415
3.393GluThr: 3.393 ± 0.386
4.788GluVal: 4.788 ± 0.415
1.162GluTrp: 1.162 ± 0.234
1.209GluTyr: 1.209 ± 0.245
0.0GluXaa: 0.0 ± 0.0
Phe
3.068PheAla: 3.068 ± 0.303
0.465PheCys: 0.465 ± 0.142
2.324PheAsp: 2.324 ± 0.359
2.417PheGlu: 2.417 ± 0.334
0.93PhePhe: 0.93 ± 0.237
2.557PheGly: 2.557 ± 0.355
0.79PheHis: 0.79 ± 0.16
1.302PheIle: 1.302 ± 0.287
0.93PheLys: 0.93 ± 0.201
2.882PheLeu: 2.882 ± 0.39
0.465PheMet: 0.465 ± 0.121
0.976PheAsn: 0.976 ± 0.174
1.58PhePro: 1.58 ± 0.272
0.744PheGln: 0.744 ± 0.152
2.557PheArg: 2.557 ± 0.371
1.766PheSer: 1.766 ± 0.286
2.231PheThr: 2.231 ± 0.318
1.859PheVal: 1.859 ± 0.34
0.79PheTrp: 0.79 ± 0.18
0.79PheTyr: 0.79 ± 0.188
0.0PheXaa: 0.0 ± 0.0
Gly
7.158GlyAla: 7.158 ± 0.907
0.79GlyCys: 0.79 ± 0.241
5.717GlyAsp: 5.717 ± 0.476
4.695GlyGlu: 4.695 ± 0.47
2.603GlyPhe: 2.603 ± 0.36
8.042GlyGly: 8.042 ± 1.23
1.487GlyHis: 1.487 ± 0.282
4.276GlyIle: 4.276 ± 0.648
3.765GlyLys: 3.765 ± 0.44
6.043GlyLeu: 6.043 ± 0.681
2.464GlyMet: 2.464 ± 0.374
2.464GlyAsn: 2.464 ± 0.364
4.369GlyPro: 4.369 ± 0.496
3.393GlyGln: 3.393 ± 0.403
5.903GlyArg: 5.903 ± 0.382
6.368GlySer: 6.368 ± 0.736
5.532GlyThr: 5.532 ± 0.716
5.764GlyVal: 5.764 ± 0.58
1.348GlyTrp: 1.348 ± 0.206
3.393GlyTyr: 3.393 ± 0.46
0.0GlyXaa: 0.0 ± 0.0
His
1.534HisAla: 1.534 ± 0.259
0.139HisCys: 0.139 ± 0.088
0.651HisAsp: 0.651 ± 0.147
1.069HisGlu: 1.069 ± 0.194
0.604HisPhe: 0.604 ± 0.164
1.534HisGly: 1.534 ± 0.306
0.372HisHis: 0.372 ± 0.135
0.837HisIle: 0.837 ± 0.186
0.651HisLys: 0.651 ± 0.187
1.487HisLeu: 1.487 ± 0.323
0.279HisMet: 0.279 ± 0.104
0.465HisAsn: 0.465 ± 0.136
1.534HisPro: 1.534 ± 0.32
0.604HisGln: 0.604 ± 0.154
1.72HisArg: 1.72 ± 0.366
0.976HisSer: 0.976 ± 0.25
1.023HisThr: 1.023 ± 0.196
1.348HisVal: 1.348 ± 0.272
0.558HisTrp: 0.558 ± 0.128
0.837HisTyr: 0.837 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
5.764IleAla: 5.764 ± 0.554
0.325IleCys: 0.325 ± 0.146
3.533IleAsp: 3.533 ± 0.359
3.905IleGlu: 3.905 ± 0.467
1.116IlePhe: 1.116 ± 0.199
4.695IleGly: 4.695 ± 0.699
0.976IleHis: 0.976 ± 0.221
1.813IleIle: 1.813 ± 0.222
1.906IleLys: 1.906 ± 0.33
3.347IleLeu: 3.347 ± 0.431
0.837IleMet: 0.837 ± 0.184
2.231IleAsn: 2.231 ± 0.317
2.835IlePro: 2.835 ± 0.37
1.627IleGln: 1.627 ± 0.303
2.696IleArg: 2.696 ± 0.33
2.696IleSer: 2.696 ± 0.339
3.161IleThr: 3.161 ± 0.391
3.3IleVal: 3.3 ± 0.455
0.93IleTrp: 0.93 ± 0.214
1.348IleTyr: 1.348 ± 0.299
0.0IleXaa: 0.0 ± 0.0
Lys
3.533LysAla: 3.533 ± 0.458
0.418LysCys: 0.418 ± 0.139
2.975LysAsp: 2.975 ± 0.387
2.092LysGlu: 2.092 ± 0.294
1.441LysPhe: 1.441 ± 0.225
2.743LysGly: 2.743 ± 0.397
0.651LysHis: 0.651 ± 0.182
1.906LysIle: 1.906 ± 0.286
2.789LysLys: 2.789 ± 0.374
3.533LysLeu: 3.533 ± 0.368
1.069LysMet: 1.069 ± 0.227
1.348LysAsn: 1.348 ± 0.266
2.65LysPro: 2.65 ± 0.367
1.58LysGln: 1.58 ± 0.268
3.579LysArg: 3.579 ± 0.434
2.417LysSer: 2.417 ± 0.289
2.65LysThr: 2.65 ± 0.317
3.021LysVal: 3.021 ± 0.372
0.744LysTrp: 0.744 ± 0.188
1.069LysTyr: 1.069 ± 0.235
0.0LysXaa: 0.0 ± 0.0
Leu
7.577LeuAla: 7.577 ± 0.704
1.023LeuCys: 1.023 ± 0.245
5.95LeuAsp: 5.95 ± 0.523
3.812LeuGlu: 3.812 ± 0.419
2.324LeuPhe: 2.324 ± 0.313
6.647LeuGly: 6.647 ± 0.852
1.069LeuHis: 1.069 ± 0.252
3.533LeuIle: 3.533 ± 0.52
3.068LeuLys: 3.068 ± 0.365
5.532LeuLeu: 5.532 ± 0.446
1.813LeuMet: 1.813 ± 0.246
2.557LeuAsn: 2.557 ± 0.364
5.299LeuPro: 5.299 ± 0.446
1.952LeuGln: 1.952 ± 0.297
6.415LeuArg: 6.415 ± 0.546
4.184LeuSer: 4.184 ± 0.465
5.392LeuThr: 5.392 ± 0.423
4.788LeuVal: 4.788 ± 0.468
0.976LeuTrp: 0.976 ± 0.178
1.395LeuTyr: 1.395 ± 0.282
0.0LeuXaa: 0.0 ± 0.0
Met
2.835MetAla: 2.835 ± 0.483
0.0MetCys: 0.0 ± 0.0
1.209MetAsp: 1.209 ± 0.224
1.395MetGlu: 1.395 ± 0.25
0.651MetPhe: 0.651 ± 0.176
1.302MetGly: 1.302 ± 0.282
0.418MetHis: 0.418 ± 0.146
1.069MetIle: 1.069 ± 0.236
1.162MetLys: 1.162 ± 0.213
1.534MetLeu: 1.534 ± 0.246
0.558MetMet: 0.558 ± 0.126
0.883MetAsn: 0.883 ± 0.237
1.72MetPro: 1.72 ± 0.352
0.604MetGln: 0.604 ± 0.137
1.534MetArg: 1.534 ± 0.224
1.673MetSer: 1.673 ± 0.339
2.324MetThr: 2.324 ± 0.34
0.976MetVal: 0.976 ± 0.188
0.418MetTrp: 0.418 ± 0.134
0.604MetTyr: 0.604 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
2.65AsnAla: 2.65 ± 0.35
0.418AsnCys: 0.418 ± 0.14
2.138AsnAsp: 2.138 ± 0.34
2.045AsnGlu: 2.045 ± 0.278
1.162AsnPhe: 1.162 ± 0.217
3.765AsnGly: 3.765 ± 0.361
0.418AsnHis: 0.418 ± 0.153
1.209AsnIle: 1.209 ± 0.316
1.023AsnLys: 1.023 ± 0.199
1.766AsnLeu: 1.766 ± 0.278
0.604AsnMet: 0.604 ± 0.177
0.837AsnAsn: 0.837 ± 0.181
2.371AsnPro: 2.371 ± 0.331
1.162AsnGln: 1.162 ± 0.208
2.696AsnArg: 2.696 ± 0.264
1.487AsnSer: 1.487 ± 0.253
1.859AsnThr: 1.859 ± 0.315
2.417AsnVal: 2.417 ± 0.325
0.837AsnTrp: 0.837 ± 0.199
1.069AsnTyr: 1.069 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
5.857ProAla: 5.857 ± 0.508
0.372ProCys: 0.372 ± 0.158
4.927ProAsp: 4.927 ± 0.465
4.276ProGlu: 4.276 ± 0.498
1.952ProPhe: 1.952 ± 0.237
5.067ProGly: 5.067 ± 0.493
1.069ProHis: 1.069 ± 0.211
3.068ProIle: 3.068 ± 0.342
2.789ProLys: 2.789 ± 0.389
4.091ProLeu: 4.091 ± 0.404
1.487ProMet: 1.487 ± 0.251
1.906ProAsn: 1.906 ± 0.305
3.068ProPro: 3.068 ± 0.362
1.627ProGln: 1.627 ± 0.232
4.555ProArg: 4.555 ± 0.62
2.882ProSer: 2.882 ± 0.319
4.137ProThr: 4.137 ± 0.449
4.137ProVal: 4.137 ± 0.448
0.837ProTrp: 0.837 ± 0.16
0.79ProTyr: 0.79 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
3.905GlnAla: 3.905 ± 0.507
0.511GlnCys: 0.511 ± 0.247
1.395GlnAsp: 1.395 ± 0.286
1.534GlnGlu: 1.534 ± 0.281
1.255GlnPhe: 1.255 ± 0.232
1.906GlnGly: 1.906 ± 0.19
0.186GlnHis: 0.186 ± 0.105
2.092GlnIle: 2.092 ± 0.479
1.116GlnLys: 1.116 ± 0.203
2.928GlnLeu: 2.928 ± 0.42
0.976GlnMet: 0.976 ± 0.179
1.395GlnAsn: 1.395 ± 0.329
1.952GlnPro: 1.952 ± 0.269
1.395GlnGln: 1.395 ± 0.343
3.021GlnArg: 3.021 ± 0.434
1.441GlnSer: 1.441 ± 0.31
2.185GlnThr: 2.185 ± 0.229
2.51GlnVal: 2.51 ± 0.389
0.651GlnTrp: 0.651 ± 0.177
0.604GlnTyr: 0.604 ± 0.149
0.0GlnXaa: 0.0 ± 0.0
Arg
7.949ArgAla: 7.949 ± 0.886
1.255ArgCys: 1.255 ± 0.316
5.02ArgAsp: 5.02 ± 0.637
5.02ArgGlu: 5.02 ± 0.581
2.603ArgPhe: 2.603 ± 0.421
5.764ArgGly: 5.764 ± 0.673
1.673ArgHis: 1.673 ± 0.264
3.905ArgIle: 3.905 ± 0.417
3.905ArgLys: 3.905 ± 0.315
4.927ArgLeu: 4.927 ± 0.46
1.72ArgMet: 1.72 ± 0.315
2.092ArgAsn: 2.092 ± 0.271
3.486ArgPro: 3.486 ± 0.361
2.324ArgGln: 2.324 ± 0.412
7.205ArgArg: 7.205 ± 0.799
3.207ArgSer: 3.207 ± 0.465
4.323ArgThr: 4.323 ± 0.461
5.625ArgVal: 5.625 ± 0.556
1.487ArgTrp: 1.487 ± 0.24
2.138ArgTyr: 2.138 ± 0.284
0.0ArgXaa: 0.0 ± 0.0
Ser
6.182SerAla: 6.182 ± 0.581
0.558SerCys: 0.558 ± 0.176
3.672SerAsp: 3.672 ± 0.395
2.882SerGlu: 2.882 ± 0.371
1.441SerPhe: 1.441 ± 0.345
5.485SerGly: 5.485 ± 0.503
0.976SerHis: 0.976 ± 0.242
2.557SerIle: 2.557 ± 0.347
1.999SerLys: 1.999 ± 0.304
3.812SerLeu: 3.812 ± 0.332
1.441SerMet: 1.441 ± 0.256
1.813SerAsn: 1.813 ± 0.294
3.021SerPro: 3.021 ± 0.362
2.138SerGln: 2.138 ± 0.36
3.393SerArg: 3.393 ± 0.429
3.161SerSer: 3.161 ± 0.476
3.951SerThr: 3.951 ± 0.48
4.323SerVal: 4.323 ± 0.469
1.116SerTrp: 1.116 ± 0.209
0.93SerTyr: 0.93 ± 0.188
0.0SerXaa: 0.0 ± 0.0
Thr
5.485ThrAla: 5.485 ± 0.573
0.279ThrCys: 0.279 ± 0.116
3.905ThrAsp: 3.905 ± 0.394
3.207ThrGlu: 3.207 ± 0.402
1.906ThrPhe: 1.906 ± 0.26
6.74ThrGly: 6.74 ± 0.713
1.023ThrHis: 1.023 ± 0.224
3.858ThrIle: 3.858 ± 0.455
2.928ThrLys: 2.928 ± 0.375
4.741ThrLeu: 4.741 ± 0.413
1.023ThrMet: 1.023 ± 0.197
1.766ThrAsn: 1.766 ± 0.273
4.369ThrPro: 4.369 ± 0.428
1.673ThrGln: 1.673 ± 0.273
4.184ThrArg: 4.184 ± 0.43
2.882ThrSer: 2.882 ± 0.325
3.951ThrThr: 3.951 ± 0.45
5.067ThrVal: 5.067 ± 0.482
1.209ThrTrp: 1.209 ± 0.195
2.045ThrTyr: 2.045 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
6.601ValAla: 6.601 ± 0.57
0.744ValCys: 0.744 ± 0.214
4.927ValAsp: 4.927 ± 0.444
5.02ValGlu: 5.02 ± 0.491
2.278ValPhe: 2.278 ± 0.338
5.996ValGly: 5.996 ± 0.636
1.441ValHis: 1.441 ± 0.284
3.161ValIle: 3.161 ± 0.304
2.045ValLys: 2.045 ± 0.305
4.927ValLeu: 4.927 ± 0.442
1.441ValMet: 1.441 ± 0.29
2.417ValAsn: 2.417 ± 0.308
4.602ValPro: 4.602 ± 0.487
2.045ValGln: 2.045 ± 0.275
5.671ValArg: 5.671 ± 0.534
4.974ValSer: 4.974 ± 0.48
4.788ValThr: 4.788 ± 0.442
5.485ValVal: 5.485 ± 0.537
1.255ValTrp: 1.255 ± 0.253
1.906ValTyr: 1.906 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
1.999TrpAla: 1.999 ± 0.35
0.279TrpCys: 0.279 ± 0.112
1.255TrpAsp: 1.255 ± 0.227
1.302TrpGlu: 1.302 ± 0.251
0.511TrpPhe: 0.511 ± 0.152
1.162TrpGly: 1.162 ± 0.213
0.418TrpHis: 0.418 ± 0.145
0.976TrpIle: 0.976 ± 0.19
0.744TrpLys: 0.744 ± 0.187
1.58TrpLeu: 1.58 ± 0.28
0.465TrpMet: 0.465 ± 0.139
0.651TrpAsn: 0.651 ± 0.2
0.79TrpPro: 0.79 ± 0.197
0.744TrpGln: 0.744 ± 0.197
1.348TrpArg: 1.348 ± 0.296
1.162TrpSer: 1.162 ± 0.297
1.116TrpThr: 1.116 ± 0.24
1.441TrpVal: 1.441 ± 0.207
0.93TrpTrp: 0.93 ± 0.219
0.418TrpTyr: 0.418 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.51TyrAla: 2.51 ± 0.298
0.325TyrCys: 0.325 ± 0.146
1.58TyrAsp: 1.58 ± 0.275
1.534TyrGlu: 1.534 ± 0.269
0.697TyrPhe: 0.697 ± 0.178
2.696TyrGly: 2.696 ± 0.416
0.372TyrHis: 0.372 ± 0.163
0.883TyrIle: 0.883 ± 0.228
0.604TyrLys: 0.604 ± 0.157
2.603TyrLeu: 2.603 ± 0.335
0.418TyrMet: 0.418 ± 0.118
0.883TyrAsn: 0.883 ± 0.198
2.045TyrPro: 2.045 ± 0.335
0.558TyrGln: 0.558 ± 0.171
2.603TyrArg: 2.603 ± 0.354
1.348TyrSer: 1.348 ± 0.233
1.302TyrThr: 1.302 ± 0.21
1.766TyrVal: 1.766 ± 0.327
0.697TyrTrp: 0.697 ± 0.257
0.697TyrTyr: 0.697 ± 0.226
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (21514 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski