Colletotrichum higginsianum non-segmented dsRNA virus 1

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7A5G0|A0A0N7A5G0_9VIRU RNA-dependent RNA polymerase OS=Colletotrichum higginsianum non-segmented dsRNA virus 1 OX=1565088 PE=4 SV=1
MM1 pKa = 7.52SAPKK5 pKa = 9.91PGASAEE11 pKa = 4.11AKK13 pKa = 9.55PVAGSLPAAGTPAVKK28 pKa = 9.18GTQSKK33 pKa = 10.12EE34 pKa = 4.2SPPAPAPLLGAVAEE48 pKa = 4.32EE49 pKa = 4.15QDD51 pKa = 4.27FVSAPQTPPPLDD63 pKa = 3.3PTRR66 pKa = 11.84FTLSEE71 pKa = 3.67NYY73 pKa = 10.01ARR75 pKa = 11.84LFCEE79 pKa = 3.96RR80 pKa = 11.84NSRR83 pKa = 11.84LAIGDD88 pKa = 4.06IIRR91 pKa = 11.84RR92 pKa = 11.84WLLSKK97 pKa = 11.11GITQGPATPASIYY110 pKa = 9.69PPGWRR115 pKa = 11.84VGRR118 pKa = 11.84TPIPVEE124 pKa = 3.98GGGVGVRR131 pKa = 11.84TLDD134 pKa = 3.24EE135 pKa = 4.11VLPNLRR141 pKa = 11.84AYY143 pKa = 10.63DD144 pKa = 3.7EE145 pKa = 4.4VGNEE149 pKa = 3.95VFVVQPEE156 pKa = 4.54GNWTRR161 pKa = 11.84VARR164 pKa = 11.84LVAVWPAPVTIVAGMLSALQDD185 pKa = 3.51TRR187 pKa = 11.84VTTDD191 pKa = 2.84RR192 pKa = 11.84VLGLAQRR199 pKa = 11.84SPEE202 pKa = 3.88ALEE205 pKa = 3.87KK206 pKa = 10.89LKK208 pKa = 11.04AAGAEE213 pKa = 4.6FKK215 pKa = 11.11AHH217 pKa = 5.02VQRR220 pKa = 11.84FLDD223 pKa = 4.02PVALRR228 pKa = 11.84RR229 pKa = 11.84SPEE232 pKa = 3.87FVEE235 pKa = 4.63VSQRR239 pKa = 11.84YY240 pKa = 8.9KK241 pKa = 10.72RR242 pKa = 11.84EE243 pKa = 3.58LRR245 pKa = 11.84ALIDD249 pKa = 3.5EE250 pKa = 4.24QQRR253 pKa = 11.84AIRR256 pKa = 11.84EE257 pKa = 3.89ARR259 pKa = 11.84AATRR263 pKa = 11.84RR264 pKa = 11.84VNQLLGEE271 pKa = 4.34RR272 pKa = 11.84DD273 pKa = 3.24QALARR278 pKa = 11.84LDD280 pKa = 3.48PAYY283 pKa = 10.64VPKK286 pKa = 10.68RR287 pKa = 11.84ATAAAALAQYY297 pKa = 10.58GIDD300 pKa = 3.43LGEE303 pKa = 4.33EE304 pKa = 4.56GEE306 pKa = 4.49VAGLEE311 pKa = 4.08EE312 pKa = 5.29SEE314 pKa = 4.46TADD317 pKa = 3.95VLATLDD323 pKa = 3.65FF324 pKa = 4.82

Molecular weight: 34.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7A5G0|A0A0N7A5G0_9VIRU RNA-dependent RNA polymerase OS=Colletotrichum higginsianum non-segmented dsRNA virus 1 OX=1565088 PE=4 SV=1
MM1 pKa = 7.46TEE3 pKa = 3.68ASGLGVKK10 pKa = 9.74DD11 pKa = 2.9WSFFLEE17 pKa = 3.97PAFKK21 pKa = 10.46SKK23 pKa = 9.32EE24 pKa = 3.96LRR26 pKa = 11.84GVRR29 pKa = 11.84CLGFSKK35 pKa = 10.48GLKK38 pKa = 8.88YY39 pKa = 10.58KK40 pKa = 10.44YY41 pKa = 9.73VQPQTKK47 pKa = 9.72NSQFFLSFLDD57 pKa = 3.38QCTMEE62 pKa = 4.51LAGIPGPSLEE72 pKa = 4.41ITTSEE77 pKa = 4.12YY78 pKa = 11.04VPPSEE83 pKa = 4.67SDD85 pKa = 3.41LFAHH89 pKa = 6.94IAGFGEE95 pKa = 4.6SGSSGSQLSNRR106 pKa = 11.84VYY108 pKa = 10.25AALRR112 pKa = 11.84EE113 pKa = 4.12EE114 pKa = 4.44LTMIEE119 pKa = 3.83HH120 pKa = 7.73WDD122 pKa = 3.72RR123 pKa = 11.84EE124 pKa = 4.25ITGWLDD130 pKa = 2.96PRR132 pKa = 11.84LLVDD136 pKa = 4.32IKK138 pKa = 11.23VPSQTSPGIRR148 pKa = 11.84WKK150 pKa = 10.87KK151 pKa = 10.3LGYY154 pKa = 8.14KK155 pKa = 7.97TKK157 pKa = 10.78KK158 pKa = 8.32EE159 pKa = 4.08ALMPATMEE167 pKa = 4.03AAKK170 pKa = 10.57VLLSMIEE177 pKa = 3.88TGTEE181 pKa = 4.23YY182 pKa = 10.41EE183 pKa = 4.34VPPCGVAGRR192 pKa = 11.84GKK194 pKa = 10.61RR195 pKa = 11.84MDD197 pKa = 3.56MNRR200 pKa = 11.84KK201 pKa = 9.27VDD203 pKa = 3.44TGGKK207 pKa = 9.41KK208 pKa = 9.3EE209 pKa = 3.84GRR211 pKa = 11.84LIVMPDD217 pKa = 3.54LVRR220 pKa = 11.84HH221 pKa = 5.67LVGTLGSGAYY231 pKa = 7.84MSKK234 pKa = 10.36VKK236 pKa = 10.44EE237 pKa = 3.97VDD239 pKa = 3.04KK240 pKa = 11.27SQGGILLGMGPFSEE254 pKa = 5.18SYY256 pKa = 10.74QSIANWARR264 pKa = 11.84GASKK268 pKa = 10.2FVFLDD273 pKa = 4.22FKK275 pKa = 11.23KK276 pKa = 10.43FDD278 pKa = 3.43HH279 pKa = 6.42RR280 pKa = 11.84VPARR284 pKa = 11.84VLQEE288 pKa = 4.04VFKK291 pKa = 10.68HH292 pKa = 4.7IASRR296 pKa = 11.84YY297 pKa = 7.05EE298 pKa = 3.78GCPGSKK304 pKa = 10.13AYY306 pKa = 8.89WRR308 pKa = 11.84SEE310 pKa = 3.56FQQLVNTKK318 pKa = 9.77IAMPDD323 pKa = 3.03GTVYY327 pKa = 10.06QKK329 pKa = 10.96KK330 pKa = 10.09RR331 pKa = 11.84GVASGDD337 pKa = 3.07PWTSIAGSYY346 pKa = 9.19ANWMMLKK353 pKa = 10.06HH354 pKa = 5.46VCNVLGLVAKK364 pKa = 10.15IWTFGDD370 pKa = 3.47DD371 pKa = 4.1SVIALYY377 pKa = 10.7DD378 pKa = 3.46VDD380 pKa = 4.23PTLDD384 pKa = 4.07LLPLFTKK391 pKa = 10.52HH392 pKa = 5.95LASSFGMHH400 pKa = 6.18VSQEE404 pKa = 3.66KK405 pKa = 10.75SYY407 pKa = 11.71VSDD410 pKa = 3.85CLVDD414 pKa = 3.28IAEE417 pKa = 4.55DD418 pKa = 4.14PEE420 pKa = 4.63PKK422 pKa = 10.25SSGSFLSLYY431 pKa = 10.3FLQTPMGVRR440 pKa = 11.84PTRR443 pKa = 11.84PLQDD447 pKa = 3.56MYY449 pKa = 11.6EE450 pKa = 3.96MMMVPEE456 pKa = 4.77RR457 pKa = 11.84NQGTLRR463 pKa = 11.84WEE465 pKa = 4.09IVRR468 pKa = 11.84TSMAYY473 pKa = 8.65LTFYY477 pKa = 11.04YY478 pKa = 10.23NDD480 pKa = 3.45RR481 pKa = 11.84ARR483 pKa = 11.84YY484 pKa = 8.91VLEE487 pKa = 5.15EE488 pKa = 3.97YY489 pKa = 10.1WDD491 pKa = 3.58WLHH494 pKa = 6.83RR495 pKa = 11.84KK496 pKa = 9.01YY497 pKa = 10.39RR498 pKa = 11.84VPEE501 pKa = 3.87LKK503 pKa = 11.06GNFTDD508 pKa = 5.06LNLLRR513 pKa = 11.84EE514 pKa = 4.05MDD516 pKa = 4.02IPWSSFKK523 pKa = 11.3LEE525 pKa = 3.88WLVRR529 pKa = 11.84LPRR532 pKa = 11.84PGEE535 pKa = 3.85VEE537 pKa = 4.5LMYY540 pKa = 10.61KK541 pKa = 10.19YY542 pKa = 10.78GHH544 pKa = 6.54TGFFPPLLWGHH555 pKa = 6.64WYY557 pKa = 10.37ARR559 pKa = 11.84YY560 pKa = 10.17DD561 pKa = 3.89RR562 pKa = 11.84DD563 pKa = 3.56VHH565 pKa = 6.22GNNVSFAFQEE575 pKa = 4.46PPTT578 pKa = 4.09

Molecular weight: 65.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

902

324

578

451.0

50.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.647 ± 3.47

0.776 ± 0.277

4.767 ± 0.264

6.984 ± 0.616

3.991 ± 0.902

7.871 ± 0.275

1.441 ± 0.671

3.215 ± 0.076

5.322 ± 1.324

9.867 ± 0.006

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 1.276

2.106 ± 0.151

6.984 ± 1.165

3.437 ± 0.524

6.763 ± 1.296

6.652 ± 1.198

5.1 ± 0.27

7.539 ± 0.653

2.217 ± 0.582

3.548 ± 1.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski