Polaromonas sp. C04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Polaromonas; unclassified Polaromonas

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S8FRY6|A0A1S8FRY6_9BURK Two-component system response regulator OmpR OS=Polaromonas sp. C04 OX=1945857 GN=B0E49_14465 PE=4 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.4NIQTQMPDD14 pKa = 3.66PIIFTDD20 pKa = 3.57SAAAKK25 pKa = 10.02VADD28 pKa = 5.45LIAEE32 pKa = 4.29EE33 pKa = 4.95GNPEE37 pKa = 3.75LKK39 pKa = 10.68LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.9GFTFDD59 pKa = 3.93EE60 pKa = 4.69VTNEE64 pKa = 4.88DD65 pKa = 4.12DD66 pKa = 3.37TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 3.79IDD92 pKa = 3.91YY93 pKa = 11.36KK94 pKa = 11.19EE95 pKa = 4.67DD96 pKa = 3.23LQGAQFVIKK105 pKa = 10.72NPGATSTCGCGSSFSAA121 pKa = 4.79

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S8FTA7|A0A1S8FTA7_9BURK D-amino-acid oxidase OS=Polaromonas sp. C04 OX=1945857 GN=B0E49_11910 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3837

0

3837

1239823

32

2890

323.1

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.699 ± 0.052

0.964 ± 0.013

5.09 ± 0.025

4.991 ± 0.036

3.615 ± 0.021

8.391 ± 0.037

2.294 ± 0.018

4.595 ± 0.029

3.459 ± 0.032

10.768 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.017

2.684 ± 0.025

5.214 ± 0.026

4.063 ± 0.023

6.611 ± 0.038

5.346 ± 0.033

5.223 ± 0.032

7.69 ± 0.036

1.454 ± 0.019

2.326 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski