Paenibacillus flagellatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V5JVP3|A0A2V5JVP3_9BACL SAM-dependent methyltransferase OS=Paenibacillus flagellatus OX=2211139 GN=DLM86_30170 PE=4 SV=1
MM1 pKa = 6.56TQAPIYY7 pKa = 9.44FQYY10 pKa = 11.03EE11 pKa = 3.89DD12 pKa = 4.17ARR14 pKa = 11.84AADD17 pKa = 3.64IAIDD21 pKa = 3.71TLSEE25 pKa = 4.04LGYY28 pKa = 9.99HH29 pKa = 5.2VHH31 pKa = 7.2PFEE34 pKa = 4.69QDD36 pKa = 2.94GKK38 pKa = 9.52PGLRR42 pKa = 11.84LYY44 pKa = 10.84VDD46 pKa = 4.41HH47 pKa = 7.56NDD49 pKa = 3.23LTSALEE55 pKa = 3.72IAQANGGTLLDD66 pKa = 3.86TDD68 pKa = 4.86CAPSEE73 pKa = 3.96ATSYY77 pKa = 11.52DD78 pKa = 3.27EE79 pKa = 5.2AYY81 pKa = 10.76GMDD84 pKa = 3.62AVRR87 pKa = 11.84IPAHH91 pKa = 6.33LVNEE95 pKa = 4.72DD96 pKa = 2.95WPEE99 pKa = 3.89TYY101 pKa = 10.28FDD103 pKa = 3.93GGGPVPGTIGSADD116 pKa = 3.66EE117 pKa = 4.73NGADD121 pKa = 3.64PAMFDD126 pKa = 4.64PSGDD130 pKa = 4.01DD131 pKa = 3.33YY132 pKa = 12.08DD133 pKa = 4.34HH134 pKa = 6.97FSAGVRR140 pKa = 11.84LL141 pKa = 3.72

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V5KFR1|A0A2V5KFR1_9BACL Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase OS=Paenibacillus flagellatus OX=2211139 GN=DLM86_02695 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84RR3 pKa = 11.84ANGRR7 pKa = 11.84SGSSPAWRR15 pKa = 11.84AAPLRR20 pKa = 11.84RR21 pKa = 11.84SRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84SRR27 pKa = 11.84APPPSPSRR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84AGRR41 pKa = 11.84PLLFGRR47 pKa = 11.84AIRR50 pKa = 11.84RR51 pKa = 11.84GRR53 pKa = 11.84ASARR57 pKa = 11.84PGG59 pKa = 3.12

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6152

0

6152

2115911

26

3527

343.9

38.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.865 ± 0.047

0.711 ± 0.009

5.516 ± 0.026

6.465 ± 0.034

4.034 ± 0.025

8.142 ± 0.033

1.941 ± 0.014

5.452 ± 0.029

4.617 ± 0.03

9.796 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.018

3.15 ± 0.021

4.658 ± 0.022

3.037 ± 0.019

6.505 ± 0.038

5.754 ± 0.024

5.401 ± 0.032

7.624 ± 0.028

1.374 ± 0.013

3.395 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski