Streptococcus satellite phage Javan625

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZXQ8|A0A4D5ZXQ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan625 OX=2558792 GN=JavanS625_0012 PE=4 SV=1
MM1 pKa = 7.6IYY3 pKa = 10.43KK4 pKa = 10.12EE5 pKa = 4.04NFMVAGISYY14 pKa = 10.4RR15 pKa = 11.84KK16 pKa = 10.01ANLRR20 pKa = 11.84KK21 pKa = 9.05IIEE24 pKa = 4.13WLEE27 pKa = 3.41YY28 pKa = 10.26DD29 pKa = 3.93YY30 pKa = 11.72EE31 pKa = 4.35FDD33 pKa = 3.72RR34 pKa = 11.84YY35 pKa = 10.31EE36 pKa = 4.54DD37 pKa = 3.51NGYY40 pKa = 8.06LTPIKK45 pKa = 10.47SEE47 pKa = 4.34YY48 pKa = 7.29YY49 pKa = 7.89TRR51 pKa = 11.84NVVLEE56 pKa = 4.22RR57 pKa = 11.84EE58 pKa = 4.41PEE60 pKa = 3.91NEE62 pKa = 3.5FDD64 pKa = 3.65KK65 pKa = 11.51NAIKK69 pKa = 10.88VLVGKK74 pKa = 7.37TQLGYY79 pKa = 10.45VPKK82 pKa = 10.64KK83 pKa = 9.61IARR86 pKa = 11.84KK87 pKa = 7.47MAKK90 pKa = 10.41LLDD93 pKa = 3.64NNINYY98 pKa = 9.6IADD101 pKa = 4.1LDD103 pKa = 4.45LDD105 pKa = 3.76EE106 pKa = 6.5DD107 pKa = 4.8YY108 pKa = 11.66NGNLNTATVNIILYY122 pKa = 10.35DD123 pKa = 3.91SEE125 pKa = 4.63TLPDD129 pKa = 4.03SPPEE133 pKa = 4.42KK134 pKa = 10.01IQNLSGEE141 pKa = 4.37IISLKK146 pKa = 10.29KK147 pKa = 10.67EE148 pKa = 3.9EE149 pKa = 4.05IEE151 pKa = 4.02IPNVEE156 pKa = 4.01IVSIEE161 pKa = 4.13DD162 pKa = 3.4LNKK165 pKa = 10.78DD166 pKa = 3.12IEE168 pKa = 4.59EE169 pKa = 4.18KK170 pKa = 11.01NKK172 pKa = 10.95AEE174 pKa = 4.11LQRR177 pKa = 11.84ASIGCLLLVIFICVIFYY194 pKa = 10.82AIFNN198 pKa = 4.21

Molecular weight: 23.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZSX4|A0A4D5ZSX4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan625 OX=2558792 GN=JavanS625_0008 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 9.25ITEE5 pKa = 4.15YY6 pKa = 10.6KK7 pKa = 9.67KK8 pKa = 10.16KK9 pKa = 10.54NGAIVYY15 pKa = 8.5RR16 pKa = 11.84AQVYY20 pKa = 10.49LGVDD24 pKa = 3.34VVTGKK29 pKa = 8.87EE30 pKa = 3.87VKK32 pKa = 9.43TRR34 pKa = 11.84ITARR38 pKa = 11.84TKK40 pKa = 10.91KK41 pKa = 9.72EE42 pKa = 3.87LKK44 pKa = 10.57LLAKK48 pKa = 10.12QNQNEE53 pKa = 4.24FVRR56 pKa = 11.84NGSTVHH62 pKa = 7.21SEE64 pKa = 4.34VKK66 pKa = 9.71VKK68 pKa = 10.54NYY70 pKa = 10.6KK71 pKa = 9.93EE72 pKa = 4.71LADD75 pKa = 4.41LWWDD79 pKa = 3.71SYY81 pKa = 11.62KK82 pKa = 10.31STVKK86 pKa = 10.31PNTVGSVALLLKK98 pKa = 10.41NHH100 pKa = 7.59IIPTFGDD107 pKa = 3.46YY108 pKa = 10.96KK109 pKa = 10.77LNKK112 pKa = 8.72LTTPLIQNKK121 pKa = 7.1VNKK124 pKa = 8.36WANKK128 pKa = 9.46ANNNEE133 pKa = 3.97PNAFIHH139 pKa = 6.12YY140 pKa = 9.1DD141 pKa = 3.33KK142 pKa = 11.34LHH144 pKa = 6.5ALNKK148 pKa = 10.21RR149 pKa = 11.84ILQYY153 pKa = 11.19GVTLQVIKK161 pKa = 10.82SNPARR166 pKa = 11.84DD167 pKa = 3.44VVLPRR172 pKa = 11.84KK173 pKa = 9.69AKK175 pKa = 9.9RR176 pKa = 11.84DD177 pKa = 3.46KK178 pKa = 11.17TKK180 pKa = 10.55IKK182 pKa = 10.86YY183 pKa = 10.13LDD185 pKa = 3.6NQEE188 pKa = 4.84LKK190 pKa = 10.24TFLNYY195 pKa = 10.38LDD197 pKa = 4.79NLNQAKK203 pKa = 9.76YY204 pKa = 9.18RR205 pKa = 11.84YY206 pKa = 8.66CFEE209 pKa = 3.79VTLYY213 pKa = 11.06KK214 pKa = 10.8LLLATGCRR222 pKa = 11.84INEE225 pKa = 4.16LLALDD230 pKa = 3.62WSDD233 pKa = 3.3IDD235 pKa = 5.14FEE237 pKa = 4.52NSCLSITKK245 pKa = 8.5TVNRR249 pKa = 11.84YY250 pKa = 9.54GGLNTPKK257 pKa = 9.73SQASIRR263 pKa = 11.84TIDD266 pKa = 3.35IDD268 pKa = 3.57KK269 pKa = 9.93ATVLMLKK276 pKa = 9.72QYY278 pKa = 10.94RR279 pKa = 11.84NRR281 pKa = 11.84QRR283 pKa = 11.84LQGLEE288 pKa = 3.72IGFVPDD294 pKa = 3.24VVFSDD299 pKa = 5.69FIHH302 pKa = 7.69DD303 pKa = 3.71RR304 pKa = 11.84TNDD307 pKa = 3.23KK308 pKa = 9.43TLSTRR313 pKa = 11.84LNTHH317 pKa = 6.44FKK319 pKa = 10.1RR320 pKa = 11.84AGVSKK325 pKa = 10.88VGFHH329 pKa = 6.84GFRR332 pKa = 11.84HH333 pKa = 4.86THH335 pKa = 6.74ASLLLNAGIPYY346 pKa = 10.01KK347 pKa = 10.28EE348 pKa = 3.98LQHH351 pKa = 6.73RR352 pKa = 11.84LGHH355 pKa = 5.56STLAMTMDD363 pKa = 4.54TYY365 pKa = 11.8SHH367 pKa = 7.14LSKK370 pKa = 10.81EE371 pKa = 4.43SAKK374 pKa = 10.48KK375 pKa = 9.98AVSFFEE381 pKa = 4.35TAVSSLL387 pKa = 3.45

Molecular weight: 44.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2963

37

471

197.5

23.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.737 ± 0.43

0.405 ± 0.109

6.311 ± 0.665

8.269 ± 0.86

4.32 ± 0.401

3.915 ± 0.344

1.35 ± 0.309

7.526 ± 0.669

9.416 ± 0.704

10.496 ± 0.81

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.327

6.817 ± 0.635

2.565 ± 0.263

4.084 ± 0.433

4.725 ± 0.427

5.029 ± 0.545

5.062 ± 0.639

5.974 ± 0.517

1.35 ± 0.174

4.59 ± 0.529
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski