Calothrix sp. PCC 6303

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; unclassified Calothrix

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9V627|K9V627_9CYAN Uncharacterized protein OS=Calothrix sp. PCC 6303 OX=1170562 GN=Cal6303_4250 PE=4 SV=1
MM1 pKa = 8.06SLEE4 pKa = 4.09QVNAFYY10 pKa = 11.03DD11 pKa = 3.91VLSSDD16 pKa = 3.25QVIYY20 pKa = 10.87DD21 pKa = 3.97EE22 pKa = 4.82YY23 pKa = 11.47CNKK26 pKa = 10.12CCLRR30 pKa = 11.84GLFGIWNWDD39 pKa = 2.96KK40 pKa = 11.79AKK42 pKa = 10.53IVDD45 pKa = 4.96FAASLGFIFTTDD57 pKa = 3.77DD58 pKa = 3.48LNMVLFEE65 pKa = 4.44GDD67 pKa = 3.27AGVVQPSMSVSVNN80 pKa = 3.07

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9V5C6|K9V5C6_9CYAN Uncharacterized protein OS=Calothrix sp. PCC 6303 OX=1170562 GN=Cal6303_4392 PE=3 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTCRR10 pKa = 11.84KK11 pKa = 9.61RR12 pKa = 11.84KK13 pKa = 7.61RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.3GRR39 pKa = 11.84HH40 pKa = 4.82RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5484

0

5484

1811230

28

2083

330.3

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.64 ± 0.03

0.994 ± 0.011

4.916 ± 0.022

6.256 ± 0.037

4.004 ± 0.02

6.59 ± 0.039

1.746 ± 0.016

7.219 ± 0.032

5.362 ± 0.034

10.651 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.014

4.923 ± 0.029

4.43 ± 0.025

5.305 ± 0.031

4.81 ± 0.027

6.747 ± 0.027

5.705 ± 0.028

6.414 ± 0.028

1.365 ± 0.015

3.054 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski