Robinsoniella sp. RHS

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Robinsoniella; unclassified Robinsoniella

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1G1A8|A0A0J1G1A8_9FIRM N-(5'-phosphoribosyl)anthranilate isomerase OS=Robinsoniella sp. RHS OX=1504536 GN=trpF PE=3 SV=1
MM1 pKa = 6.03VTAGIGFQHH10 pKa = 6.24FVDD13 pKa = 4.68GSEE16 pKa = 4.45SKK18 pKa = 11.03NVTNNRR24 pKa = 11.84NTVQAAGLTILEE36 pKa = 4.79PEE38 pKa = 4.54DD39 pKa = 3.52TTSADD44 pKa = 3.03ISLLASVEE52 pKa = 4.1EE53 pKa = 4.47SNEE56 pKa = 3.55DD57 pKa = 4.19LINKK61 pKa = 7.97NISWEE66 pKa = 4.01EE67 pKa = 3.98LEE69 pKa = 6.15LNLQSCLNGYY79 pKa = 10.3DD80 pKa = 4.47GDD82 pKa = 3.51WSLYY86 pKa = 10.34IKK88 pKa = 10.67DD89 pKa = 3.76LAEE92 pKa = 4.57DD93 pKa = 3.8RR94 pKa = 11.84SISLNSHH101 pKa = 5.57EE102 pKa = 4.51MQAASLIKK110 pKa = 10.07LYY112 pKa = 10.74IMGAVYY118 pKa = 9.76QQAEE122 pKa = 4.43EE123 pKa = 4.69GGLILDD129 pKa = 4.62GNQIAEE135 pKa = 4.63EE136 pKa = 4.55LEE138 pKa = 3.99QMITISDD145 pKa = 3.74NEE147 pKa = 4.35SANEE151 pKa = 3.81LVRR154 pKa = 11.84EE155 pKa = 4.5LEE157 pKa = 4.34PEE159 pKa = 4.18SEE161 pKa = 4.67FADD164 pKa = 3.93CLEE167 pKa = 4.21KK168 pKa = 11.04VNNYY172 pKa = 9.79IEE174 pKa = 4.27TAGFSDD180 pKa = 3.39TRR182 pKa = 11.84QINGIGDD189 pKa = 3.35VSLWSGEE196 pKa = 4.16GVNTTSVNDD205 pKa = 3.65CGEE208 pKa = 4.17FLEE211 pKa = 4.76SVYY214 pKa = 11.4NKK216 pKa = 9.56TLVSEE221 pKa = 4.27EE222 pKa = 4.52ASNEE226 pKa = 4.0MLSLLLQQEE235 pKa = 4.19VDD237 pKa = 3.7YY238 pKa = 10.47KK239 pKa = 10.77IPEE242 pKa = 4.3SLPSDD247 pKa = 3.39VKK249 pKa = 10.86VANKK253 pKa = 9.45TGEE256 pKa = 4.06TDD258 pKa = 3.95DD259 pKa = 5.34CEE261 pKa = 4.16NDD263 pKa = 3.52CAIVYY268 pKa = 10.41SDD270 pKa = 3.37GGDD273 pKa = 3.65YY274 pKa = 10.37IICVMSEE281 pKa = 3.32GWTSDD286 pKa = 3.39DD287 pKa = 3.49EE288 pKa = 4.58AVANIQNISQMVYY301 pKa = 10.7GYY303 pKa = 9.8FNPP306 pKa = 4.04

Molecular weight: 33.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1FZ15|A0A0J1FZ15_9FIRM N-acylglucosamine-6-phosphate 2-epimerase OS=Robinsoniella sp. RHS OX=1504536 GN=RHS_5683 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTVGGRR28 pKa = 11.84KK29 pKa = 9.24VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6119

0

6119

2057799

37

7662

336.3

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.119 ± 0.029

1.421 ± 0.014

5.534 ± 0.025

7.297 ± 0.036

4.268 ± 0.024

7.135 ± 0.034

1.655 ± 0.015

7.693 ± 0.037

7.071 ± 0.032

8.802 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.154 ± 0.019

4.756 ± 0.029

3.273 ± 0.017

3.446 ± 0.017

3.905 ± 0.022

6.072 ± 0.027

5.473 ± 0.037

6.57 ± 0.023

1.045 ± 0.013

4.311 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski