Schizopora paradoxa
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17051 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H2SJU8|A0A0H2SJU8_9AGAM Peptidase_M48 domain-containing protein OS=Schizopora paradoxa OX=27342 GN=SCHPADRAFT_937090 PE=4 SV=1
MM1 pKa = 7.03 YY2 pKa = 10.56 AFGGFVYY9 pKa = 10.45 SLSSCPSAEE18 pKa = 3.85 TMASDD23 pKa = 4.29 FSTMKK28 pKa = 10.5 SVGSRR33 pKa = 11.84 IVVTFDD39 pKa = 2.98 PCEE42 pKa = 4.57 DD43 pKa = 3.91 GSEE46 pKa = 3.89 DD47 pKa = 3.7 TYY49 pKa = 11.98 DD50 pKa = 3.58 NVFAAAGAAGIYY62 pKa = 8.26 VIPIAWTLVDD72 pKa = 4.08 SGTFTSEE79 pKa = 3.84 SVPRR83 pKa = 11.84 LEE85 pKa = 4.61 AVTDD89 pKa = 3.93 AVIKK93 pKa = 10.82 NPSNVIAVAYY103 pKa = 10.08 GDD105 pKa = 4.58 EE106 pKa = 4.49 PLLDD110 pKa = 5.2 DD111 pKa = 5.48 DD112 pKa = 5.94 AGSASNLATYY122 pKa = 7.44 VTQMKK127 pKa = 10.58 SEE129 pKa = 4.07 FVNAGLDD136 pKa = 3.52 MPVSISEE143 pKa = 4.34 MAGGWQNSANGDD155 pKa = 3.4 ISSMVAAIDD164 pKa = 3.6 FFMVNDD170 pKa = 3.78 MPYY173 pKa = 10.02 FFPDD177 pKa = 2.95 ATTGGSSGSWNDD189 pKa = 3.3 FVSDD193 pKa = 3.99 LEE195 pKa = 4.54 YY196 pKa = 10.49 IASIANGKK204 pKa = 9.42 HH205 pKa = 5.47 IMVTQTGWPSNEE217 pKa = 4.82 DD218 pKa = 3.03 EE219 pKa = 4.72 FAPNSNSIDD228 pKa = 3.28 ASVSSEE234 pKa = 3.71 AAFWQLLDD242 pKa = 3.77 SHH244 pKa = 7.36 CSDD247 pKa = 4.05 FFKK250 pKa = 11.01 PNDD253 pKa = 3.76 IAWMWRR259 pKa = 11.84 SWDD262 pKa = 3.53 DD263 pKa = 4.98 HH264 pKa = 5.12 ITGWGVLDD272 pKa = 4.11 SNGNPKK278 pKa = 9.09 FTVQASTSCC287 pKa = 3.29
Molecular weight: 30.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.656
IPC_protein 3.681
Toseland 3.452
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.363
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.808
Patrickios 0.807
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A0H2RAV5|A0A0H2RAV5_9AGAM Mannose-P-dolichol utilization defect 1 protein homolog OS=Schizopora paradoxa OX=27342 GN=SCHPADRAFT_1002241 PE=3 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.43 RR3 pKa = 11.84 LLLLVVSMQVGKK15 pKa = 10.47 ARR17 pKa = 11.84 PSSVIRR23 pKa = 11.84 RR24 pKa = 11.84 PTRR27 pKa = 11.84 PANLLLAFTRR37 pKa = 11.84 IHH39 pKa = 6.85 PPLSSRR45 pKa = 11.84 RR46 pKa = 11.84 EE47 pKa = 3.68 RR48 pKa = 11.84 ARR50 pKa = 11.84 QRR52 pKa = 11.84 VWRR55 pKa = 11.84 MKK57 pKa = 9.91 RR58 pKa = 11.84 LHH60 pKa = 6.6 PFGSNVRR67 pKa = 11.84 PLVRR71 pKa = 11.84 PLVRR75 pKa = 11.84 PLVLHH80 pKa = 6.64 LSPSIALLPPPSAHH94 pKa = 6.4 HH95 pKa = 6.44 SSPIHH100 pKa = 6.05 HH101 pKa = 7.1 LFITTSSRR109 pKa = 11.84 ARR111 pKa = 11.84 PRR113 pKa = 11.84 PPKK116 pKa = 10.0 ASKK119 pKa = 9.44 PAPSLMPRR127 pKa = 11.84 LINGSS132 pKa = 3.44
Molecular weight: 14.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.559
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.281
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.176
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17050
1
17051
7036197
49
4931
412.7
45.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.975 ± 0.018
1.421 ± 0.008
5.676 ± 0.014
6.082 ± 0.021
4.121 ± 0.011
6.231 ± 0.015
2.445 ± 0.009
4.99 ± 0.013
4.746 ± 0.018
9.383 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.132 ± 0.007
3.679 ± 0.009
5.886 ± 0.021
3.501 ± 0.012
6.401 ± 0.017
9.246 ± 0.025
5.799 ± 0.013
6.341 ± 0.014
1.369 ± 0.007
2.578 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here