Laccaria amethystina LaAM-08-1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Tricholomataceae; Laccaria; Laccaria amethystina

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C9YDX5|A0A0C9YDX5_9AGAR Dipeptidyl peptidase 3 OS=Laccaria amethystina LaAM-08-1 OX=1095629 GN=K443DRAFT_672163 PE=3 SV=1
MM1 pKa = 7.65SSLLSLISLLSLLAAVYY18 pKa = 9.31SAPAQLSYY26 pKa = 11.41DD27 pKa = 3.5QLTPSPAAAPDD38 pKa = 3.79FLKK41 pKa = 11.12QNGLDD46 pKa = 3.62AQKK49 pKa = 10.85LNSQFASMKK58 pKa = 9.34PTDD61 pKa = 3.74SCQDD65 pKa = 3.04GQMACVTSAFAQCVGGKK82 pKa = 8.77WSLSTCSSGLACYY95 pKa = 9.93ALPLVNKK102 pKa = 9.44PGTSLVCDD110 pKa = 3.9TPMDD114 pKa = 3.73AAARR118 pKa = 11.84FAATGVDD125 pKa = 3.41GGVTGTVPPAQGNNSTSAPTPSGVDD150 pKa = 3.39DD151 pKa = 5.28PVDD154 pKa = 4.61DD155 pKa = 5.73DD156 pKa = 5.75CDD158 pKa = 5.0DD159 pKa = 4.44DD160 pKa = 4.66TSSTDD165 pKa = 4.6DD166 pKa = 4.85GDD168 pKa = 5.83GDD170 pKa = 4.31DD171 pKa = 5.05CDD173 pKa = 4.14EE174 pKa = 4.41GTPVAGAPQIPDD186 pKa = 3.68DD187 pKa = 4.34HH188 pKa = 8.39CGDD191 pKa = 5.1DD192 pKa = 4.17DD193 pKa = 4.19TTSSVPLTTPRR204 pKa = 11.84TVKK207 pKa = 10.39SASQSRR213 pKa = 11.84TVTSASSTPTIANQANEE230 pKa = 4.18PAPTTIYY237 pKa = 9.7LTSQSSASTTPSVILLNRR255 pKa = 11.84RR256 pKa = 11.84QAPSTLPTPPPATTTALTSSAPISTSSSSPAIVTPTVVIGTDD298 pKa = 4.22GIVTVTVVSTVFVSASCGLPSVSATLSSAPSAITVSSAIPPAVTLTSPAITSSSASSASITATPSATGVISLSVPPVASDD378 pKa = 3.06TSINGSAGGGLTFISQVATPPAVPSPTGSFNLSEE412 pKa = 4.67LLTATVASS420 pKa = 4.16

Molecular weight: 41.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C9WUF4|A0A0C9WUF4_9AGAR Unplaced genomic scaffold K443scaffold_52 whole genome shotgun sequence (Fragment) OS=Laccaria amethystina LaAM-08-1 OX=1095629 GN=K443DRAFT_40104 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84LMRR5 pKa = 11.84QNTLQLLIPLPMSIQRR21 pKa = 11.84LNVINPILRR30 pKa = 11.84RR31 pKa = 11.84PGLPLLHH38 pKa = 6.21GRR40 pKa = 11.84LRR42 pKa = 11.84HH43 pKa = 5.5AHH45 pKa = 5.26TLHH48 pKa = 6.41PRR50 pKa = 11.84KK51 pKa = 10.0

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20880

13

20893

6674714

46

5021

319.5

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.707 ± 0.016

1.421 ± 0.009

5.393 ± 0.013

5.749 ± 0.02

3.882 ± 0.013

6.256 ± 0.016

2.745 ± 0.009

5.055 ± 0.015

4.8 ± 0.016

9.441 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.007

3.696 ± 0.011

6.55 ± 0.024

3.81 ± 0.012

6.127 ± 0.016

8.929 ± 0.025

6.155 ± 0.013

6.188 ± 0.015

1.438 ± 0.006

2.598 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski