Candidatus Hoaglandella endobia

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Hoaglandella

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 517 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143WTV5|A0A143WTV5_9ENTR Probable peptidoglycan glycosyltransferase FtsW OS=Candidatus Hoaglandella endobia OX=1778263 GN=ftsW PE=3 SV=1
MM1 pKa = 7.88WYY3 pKa = 9.79FAWILGTMLACTGGIISALTIDD25 pKa = 4.53FFEE28 pKa = 4.87DD29 pKa = 3.43TTVVNSAVTEE39 pKa = 4.01QRR41 pKa = 3.24

Molecular weight: 4.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143WTP4|A0A143WTP4_9ENTR DNA polymerase III subunit epsilon OS=Candidatus Hoaglandella endobia OX=1778263 GN=dnaQ PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.25RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.89EE38 pKa = 3.6RR39 pKa = 11.84ARR41 pKa = 11.84LTVSSKK47 pKa = 11.16

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

511

6

517

169964

29

1436

328.8

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.301 ± 0.1

1.201 ± 0.036

4.999 ± 0.062

5.554 ± 0.103

3.638 ± 0.066

6.843 ± 0.083

2.313 ± 0.042

7.405 ± 0.08

5.35 ± 0.087

10.879 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.048

4.469 ± 0.067

3.824 ± 0.051

4.436 ± 0.061

5.855 ± 0.09

5.931 ± 0.072

5.296 ± 0.064

6.846 ± 0.08

1.08 ± 0.039

3.065 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski