Turkeypox virus
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M3ZHH4|A0A0M3ZHH4_9POXV TGF-beta-like protein OS=Turkeypox virus OX=336486 PE=3 SV=1
MM1 pKa = 7.89 LDD3 pKa = 4.07 NINVLLLVAFVSAILIVDD21 pKa = 4.08 FDD23 pKa = 3.81 IVLYY27 pKa = 10.76 AIIFYY32 pKa = 11.12 ALLLIFNIDD41 pKa = 3.41 PVISVFMLLVSLKK54 pKa = 10.82 LVISLNNLLSEE65 pKa = 4.25 YY66 pKa = 10.69 LIDD69 pKa = 3.96
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.957
IPC2_protein 3.554
IPC_protein 3.35
Toseland 3.198
ProMoST 3.49
Dawson 3.427
Bjellqvist 3.757
Wikipedia 3.49
Rodwell 3.236
Grimsley 3.134
Solomon 3.312
Lehninger 3.261
Nozaki 3.617
DTASelect 3.783
Thurlkill 3.338
EMBOSS 3.478
Sillero 3.516
Patrickios 0.299
IPC_peptide 3.3
IPC2_peptide 3.452
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A0M3ZK19|A0A0M3ZK19_9POXV Uncharacterized protein OS=Turkeypox virus OX=336486 PE=3 SV=1
MM1 pKa = 7.71 EE2 pKa = 5.09 IAKK5 pKa = 7.99 EE6 pKa = 3.97 TLIVIGLTILVIILVISGFSLILRR30 pKa = 11.84 LIPGVYY36 pKa = 10.05 SSVARR41 pKa = 11.84 SSFTGGKK48 pKa = 8.87 ILRR51 pKa = 11.84 FMEE54 pKa = 4.25 IFSTLMFIPGIIILYY69 pKa = 9.13 AAYY72 pKa = 9.38 IRR74 pKa = 11.84 KK75 pKa = 8.58 FRR77 pKa = 11.84 SGNN80 pKa = 3.29
Molecular weight: 8.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.867
IPC_protein 10.496
Toseland 10.365
ProMoST 10.116
Dawson 10.555
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.847
Grimsley 10.643
Solomon 10.628
Lehninger 10.584
Nozaki 10.321
DTASelect 10.277
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.467
Patrickios 10.657
IPC_peptide 10.613
IPC2_peptide 9.077
IPC2.peptide.svr19 8.538
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
170
0
170
55063
54
1810
323.9
37.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.356 ± 0.105
2.185 ± 0.104
6.454 ± 0.123
5.127 ± 0.106
4.444 ± 0.149
3.781 ± 0.115
1.95 ± 0.058
11.24 ± 0.156
7.824 ± 0.165
9.213 ± 0.179
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.067
7.826 ± 0.149
3.075 ± 0.106
1.823 ± 0.082
4.03 ± 0.107
7.591 ± 0.131
5.488 ± 0.103
5.376 ± 0.114
0.647 ± 0.046
5.964 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here