Seal anellovirus 6
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7TWN7|A0A0A7TWN7_9VIRU Capsid protein OS=Seal anellovirus 6 OX=1566012 PE=3 SV=1
MM1 pKa = 7.22 TKK3 pKa = 9.64 SHH5 pKa = 6.56 SGFAIVSNLRR15 pKa = 11.84 SLEE18 pKa = 4.11 TLSTEE23 pKa = 4.16 DD24 pKa = 3.43 YY25 pKa = 10.22 RR26 pKa = 11.84 RR27 pKa = 11.84 SAIQTPSLKK36 pKa = 10.42 HH37 pKa = 5.51 QEE39 pKa = 4.2 LKK41 pKa = 9.59 TKK43 pKa = 10.43 YY44 pKa = 9.95 RR45 pKa = 11.84 LAPYY49 pKa = 10.36 SKK51 pKa = 10.89 LDD53 pKa = 3.57 LSTLGTYY60 pKa = 10.81 LKK62 pKa = 9.86 GTSRR66 pKa = 11.84 TAASRR71 pKa = 11.84 KK72 pKa = 9.14 RR73 pKa = 11.84 VLEE76 pKa = 3.87 EE77 pKa = 3.77 LLDD80 pKa = 4.76 LIEE83 pKa = 5.76 EE84 pKa = 4.47 DD85 pKa = 5.1 SQPNWQTQAQDD96 pKa = 3.19 VSDD99 pKa = 4.88 FSTTPGSEE107 pKa = 4.12 SEE109 pKa = 4.3 EE110 pKa = 4.34 SDD112 pKa = 3.85 VSYY115 pKa = 11.0 LGYY118 pKa = 10.14 WSDD121 pKa = 3.69 EE122 pKa = 4.11 EE123 pKa = 4.57 NPGGEE128 pKa = 4.57 GPPLVPPTPGPKK140 pKa = 9.67 APEE143 pKa = 4.12 YY144 pKa = 9.67 PPHH147 pKa = 6.74 FLFLTLQQ154 pKa = 3.44
Molecular weight: 17.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.923
IPC2_protein 4.978
IPC_protein 4.863
Toseland 4.762
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.952
Wikipedia 4.66
Rodwell 4.736
Grimsley 4.673
Solomon 4.813
Lehninger 4.762
Nozaki 4.927
DTASelect 5.041
Thurlkill 4.749
EMBOSS 4.685
Sillero 5.003
Patrickios 4.012
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 5.015
Protein with the highest isoelectric point:
>tr|A0A0A7U2J0|A0A0A7U2J0_9VIRU ORF2 OS=Seal anellovirus 6 OX=1566012 PE=4 SV=1
MM1 pKa = 8.06 AYY3 pKa = 9.86 RR4 pKa = 11.84 HH5 pKa = 5.89 RR6 pKa = 11.84 RR7 pKa = 11.84 PFRR10 pKa = 11.84 RR11 pKa = 11.84 YY12 pKa = 8.17 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 YY16 pKa = 8.05 PRR18 pKa = 11.84 WKK20 pKa = 9.02 GRR22 pKa = 11.84 RR23 pKa = 11.84 VHH25 pKa = 6.89 RR26 pKa = 11.84 RR27 pKa = 11.84 HH28 pKa = 4.57 TWRR31 pKa = 11.84 PFHH34 pKa = 6.37 HH35 pKa = 5.93 WRR37 pKa = 11.84 RR38 pKa = 11.84 WRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 PYY44 pKa = 10.28 RR45 pKa = 11.84 RR46 pKa = 11.84 GTVAVRR52 pKa = 11.84 YY53 pKa = 6.78 TNPRR57 pKa = 11.84 RR58 pKa = 11.84 HH59 pKa = 6.27 KK60 pKa = 9.57 YY61 pKa = 9.98 ISVTGWEE68 pKa = 4.29 PLGNCCIQDD77 pKa = 3.64 KK78 pKa = 10.97 ASDD81 pKa = 3.62 EE82 pKa = 4.1 ATPYY86 pKa = 10.97 EE87 pKa = 4.74 DD88 pKa = 5.44 LDD90 pKa = 4.5 SDD92 pKa = 4.05 VQTWANRR99 pKa = 11.84 TRR101 pKa = 11.84 TAGIWHH107 pKa = 6.55 GTYY110 pKa = 9.59 GHH112 pKa = 7.25 HH113 pKa = 6.74 YY114 pKa = 8.98 PWQLLCQRR122 pKa = 11.84 AQFYY126 pKa = 10.62 FCTFSSGWKK135 pKa = 10.23 GYY137 pKa = 10.82 DD138 pKa = 3.13 YY139 pKa = 11.25 LQFYY143 pKa = 10.05 GGNIWLPKK151 pKa = 9.62 LAAPYY156 pKa = 8.73 FFWVDD161 pKa = 3.42 PTVQDD166 pKa = 4.03 PKK168 pKa = 10.34 KK169 pKa = 10.4 SSYY172 pKa = 10.87 EE173 pKa = 4.11 SLCEE177 pKa = 4.19 GQDD180 pKa = 3.51 LVPPRR185 pKa = 11.84 GIHH188 pKa = 4.62 EE189 pKa = 4.39 QKK191 pKa = 9.93 GAHH194 pKa = 6.18 YY195 pKa = 10.1 IPANNEE201 pKa = 3.53 SHH203 pKa = 5.66 TLKK206 pKa = 10.42 GYY208 pKa = 10.45 KK209 pKa = 9.49 RR210 pKa = 11.84 IHH212 pKa = 6.62 IKK214 pKa = 10.5 RR215 pKa = 11.84 LSTWEE220 pKa = 3.77 GVYY223 pKa = 10.56 AISTAADD230 pKa = 3.82 YY231 pKa = 11.22 ILVHH235 pKa = 5.98 WVWSTVTKK243 pKa = 10.65 NWAFFDD249 pKa = 4.43 GACYY253 pKa = 9.7 QAGQASSDD261 pKa = 3.96 TCNSTPWFMSAQTAEE276 pKa = 4.23 SGVKK280 pKa = 10.24 KK281 pKa = 10.57 EE282 pKa = 3.48 VDD284 pKa = 3.49 EE285 pKa = 4.28 YY286 pKa = 11.8 KK287 pKa = 9.66 KK288 pKa = 9.53 TNDD291 pKa = 3.44 FKK293 pKa = 11.82 NNLYY297 pKa = 10.4 CYY299 pKa = 8.62 PDD301 pKa = 3.97 PRR303 pKa = 11.84 ASWVNRR309 pKa = 11.84 QKK311 pKa = 11.16 YY312 pKa = 9.91 NKK314 pKa = 8.6 TIQPLTVTEE323 pKa = 4.22 NPGGPFLPSQGAGLGLSRR341 pKa = 11.84 QYY343 pKa = 11.25 DD344 pKa = 3.34 QVSFWFRR351 pKa = 11.84 YY352 pKa = 8.16 RR353 pKa = 11.84 FKK355 pKa = 10.94 FKK357 pKa = 10.99 VSGDD361 pKa = 3.35 SVYY364 pKa = 10.76 RR365 pKa = 11.84 RR366 pKa = 11.84 LPAVSNTDD374 pKa = 4.09 TIPEE378 pKa = 4.04 APGAQNQVSPRR389 pKa = 11.84 SILKK393 pKa = 10.04 ARR395 pKa = 11.84 PLDD398 pKa = 3.59 TGDD401 pKa = 3.54 ILEE404 pKa = 5.3 GDD406 pKa = 4.12 LQDD409 pKa = 3.62 GCLTEE414 pKa = 3.98 EE415 pKa = 4.06 SLRR418 pKa = 11.84 RR419 pKa = 11.84 ITGSHH424 pKa = 6.24 RR425 pKa = 11.84 RR426 pKa = 11.84 RR427 pKa = 11.84 LPTKK431 pKa = 10.27 LADD434 pKa = 3.41 SSSRR438 pKa = 11.84 RR439 pKa = 11.84 VRR441 pKa = 11.84 LFHH444 pKa = 7.55 DD445 pKa = 3.58 PGVRR449 pKa = 11.84 KK450 pKa = 9.73 RR451 pKa = 11.84 RR452 pKa = 11.84 IRR454 pKa = 11.84 RR455 pKa = 11.84 LLSGLLEE462 pKa = 4.23
Molecular weight: 54.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.859
IPC2_protein 9.545
IPC_protein 9.97
Toseland 9.97
ProMoST 9.809
Dawson 10.248
Bjellqvist 9.999
Wikipedia 10.467
Rodwell 10.438
Grimsley 10.335
Solomon 10.262
Lehninger 10.218
Nozaki 10.014
DTASelect 9.97
Thurlkill 10.072
EMBOSS 10.394
Sillero 10.16
Patrickios 5.817
IPC_peptide 10.262
IPC2_peptide 8.975
IPC2.peptide.svr19 8.566
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
714
98
462
238.0
27.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.742 ± 0.516
1.681 ± 0.672
5.602 ± 0.652
5.042 ± 1.666
3.501 ± 0.423
7.983 ± 2.126
3.501 ± 0.785
2.941 ± 0.534
5.182 ± 0.02
8.123 ± 1.732
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.56 ± 0.139
3.081 ± 0.466
6.723 ± 0.931
4.342 ± 0.09
8.543 ± 2.739
8.123 ± 1.665
7.283 ± 1.022
4.062 ± 0.971
3.081 ± 0.86
4.902 ± 1.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here