Mycoplasma phocidae
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 608 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5IQS1|A0A2Z5IQS1_9MOLU 30S ribosomal protein S7 OS=Mycoplasma phocidae OX=142651 GN=rpsG PE=3 SV=1
MM1 pKa = 7.17 SRR3 pKa = 11.84 KK4 pKa = 9.14 EE5 pKa = 3.78 INIFTEE11 pKa = 3.88 DD12 pKa = 3.19 RR13 pKa = 11.84 RR14 pKa = 11.84 IITDD18 pKa = 4.24 DD19 pKa = 3.28 GDD21 pKa = 3.85 EE22 pKa = 4.39 IYY24 pKa = 11.4 VLFDD28 pKa = 3.43 LEE30 pKa = 4.62 EE31 pKa = 5.16 NGDD34 pKa = 3.88 YY35 pKa = 11.18 YY36 pKa = 11.44 LILTDD41 pKa = 4.38 GEE43 pKa = 4.2 ALFFVKK49 pKa = 10.0 EE50 pKa = 4.15 HH51 pKa = 6.25 NGKK54 pKa = 8.3 ITEE57 pKa = 4.23 IDD59 pKa = 3.64 DD60 pKa = 3.76 EE61 pKa = 4.95 GEE63 pKa = 3.57 IDD65 pKa = 4.14 ILVDD69 pKa = 4.72 LLFKK73 pKa = 10.28 FAKK76 pKa = 10.33 DD77 pKa = 3.45 NLVLDD82 pKa = 4.85 RR83 pKa = 11.84 DD84 pKa = 3.96 QKK86 pKa = 11.7 SDD88 pKa = 4.05 LLAKK92 pKa = 10.76 LIGDD96 pKa = 4.3 DD97 pKa = 4.05 SEE99 pKa = 5.11 KK100 pKa = 11.1 SII102 pKa = 5.05
Molecular weight: 11.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.354
IPC2_protein 4.037
IPC_protein 4.012
Toseland 3.808
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.923
Rodwell 3.846
Grimsley 3.719
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.342
Thurlkill 3.846
EMBOSS 3.935
Sillero 4.139
Patrickios 3.923
IPC_peptide 3.986
IPC2_peptide 4.113
IPC2.peptide.svr19 4.058
Protein with the highest isoelectric point:
>tr|A0A2Z5IQ15|A0A2Z5IQ15_9MOLU TlyA family rRNA (Cytidine-2'-O)-methyltransferase OS=Mycoplasma phocidae OX=142651 GN=DA803_02150 PE=4 SV=1
MM1 pKa = 7.35 ARR3 pKa = 11.84 KK4 pKa = 9.72 SLMVKK9 pKa = 10.4 AEE11 pKa = 4.27 RR12 pKa = 11.84 EE13 pKa = 3.9 PKK15 pKa = 9.9 FAVRR19 pKa = 11.84 KK20 pKa = 5.95 YY21 pKa = 8.69 TRR23 pKa = 11.84 CQLCGRR29 pKa = 11.84 VHH31 pKa = 6.88 AVLRR35 pKa = 11.84 KK36 pKa = 9.58 YY37 pKa = 10.23 KK38 pKa = 9.85 ICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 KK46 pKa = 9.48 LAHH49 pKa = 6.61 EE50 pKa = 4.39 GKK52 pKa = 10.18 IPGVKK57 pKa = 9.38 KK58 pKa = 10.89 ASWW61 pKa = 3.03
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.794
IPC_protein 10.277
Toseland 10.921
ProMoST 10.496
Dawson 10.979
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.418
Grimsley 11.008
Solomon 11.082
Lehninger 11.067
Nozaki 10.906
DTASelect 10.628
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.921
Patrickios 11.155
IPC_peptide 11.096
IPC2_peptide 9.721
IPC2.peptide.svr19 8.558
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
608
0
608
226822
37
2777
373.1
42.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.222 ± 0.089
0.466 ± 0.024
5.779 ± 0.094
6.997 ± 0.097
5.434 ± 0.09
4.504 ± 0.091
1.348 ± 0.04
10.111 ± 0.103
10.729 ± 0.105
9.128 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.775 ± 0.042
8.295 ± 0.131
2.712 ± 0.057
3.041 ± 0.047
3.049 ± 0.071
6.798 ± 0.092
4.643 ± 0.066
4.986 ± 0.068
0.862 ± 0.035
4.121 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here