Streptococcus satellite phage Javan652
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZYG3|A0A4D5ZYG3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan652 OX=2558807 GN=JavanS652_0013 PE=4 SV=1
MM1 pKa = 7.1 TFKK4 pKa = 10.97 LFSDD8 pKa = 3.63 NAKK11 pKa = 10.57 SFTFKK16 pKa = 10.81 HH17 pKa = 6.35 DD18 pKa = 3.64 FEE20 pKa = 5.0 TMDD23 pKa = 3.54 HH24 pKa = 6.56 AKK26 pKa = 10.34 VSSTAILGYY35 pKa = 9.44 MVGTYY40 pKa = 7.14 EE41 pKa = 4.12 QQPTEE46 pKa = 3.74 ITIGGNEE53 pKa = 4.22 ANNTFTMVVKK63 pKa = 10.59 YY64 pKa = 10.86 VEE66 pKa = 5.52 DD67 pKa = 3.78 EE68 pKa = 4.43 DD69 pKa = 3.79 LTKK72 pKa = 10.64 VFNRR76 pKa = 11.84 ICDD79 pKa = 3.6 SFEE82 pKa = 4.4 SYY84 pKa = 11.29 SKK86 pKa = 11.06 GCSEE90 pKa = 4.49 AA91 pKa = 3.97
Molecular weight: 10.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.633
IPC2_protein 4.749
IPC_protein 4.596
Toseland 4.457
ProMoST 4.698
Dawson 4.546
Bjellqvist 4.685
Wikipedia 4.418
Rodwell 4.444
Grimsley 4.368
Solomon 4.533
Lehninger 4.495
Nozaki 4.66
DTASelect 4.8
Thurlkill 4.457
EMBOSS 4.431
Sillero 4.711
Patrickios 3.605
IPC_peptide 4.546
IPC2_peptide 4.711
IPC2.peptide.svr19 4.671
Protein with the highest isoelectric point:
>tr|A0A4D5ZU46|A0A4D5ZU46_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan652 OX=2558807 GN=JavanS652_0011 PE=4 SV=1
MM1 pKa = 7.78 KK2 pKa = 10.26 INEE5 pKa = 4.22 IKK7 pKa = 10.67 KK8 pKa = 9.76 KK9 pKa = 10.54 DD10 pKa = 3.3 GSTVYY15 pKa = 10.36 RR16 pKa = 11.84 ANIYY20 pKa = 10.62 LGVDD24 pKa = 3.26 VVTGKK29 pKa = 10.73 KK30 pKa = 7.94 VTTKK34 pKa = 9.32 VTARR38 pKa = 11.84 TKK40 pKa = 10.91 KK41 pKa = 9.48 EE42 pKa = 4.24 LKK44 pKa = 9.67 TKK46 pKa = 10.1 AQQAQFDD53 pKa = 4.54 FKK55 pKa = 11.59 ANGSTRR61 pKa = 11.84 YY62 pKa = 9.81 KK63 pKa = 10.2 EE64 pKa = 4.23 VTIEE68 pKa = 4.09 TYY70 pKa = 10.05 EE71 pKa = 4.15 ALATSWWDD79 pKa = 3.28 NYY81 pKa = 10.78 KK82 pKa = 9.05 NTVKK86 pKa = 10.75 VNTRR90 pKa = 11.84 KK91 pKa = 9.31 LQKK94 pKa = 10.61 SILDD98 pKa = 3.51 KK99 pKa = 11.1 HH100 pKa = 6.49 LLPLFGSFKK109 pKa = 10.45 LDD111 pKa = 3.12 KK112 pKa = 10.15 LTTPLIQSIMNKK124 pKa = 9.56 LANRR128 pKa = 11.84 TNTGEE133 pKa = 3.92 VGAYY137 pKa = 8.95 LHH139 pKa = 6.49 YY140 pKa = 11.1 DD141 pKa = 3.92 KK142 pKa = 11.05 IHH144 pKa = 6.58 ALNKK148 pKa = 10.18 RR149 pKa = 11.84 ILQYY153 pKa = 11.17 GVVLQVIPTNPASNVVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.1 DD177 pKa = 3.22 KK178 pKa = 10.56 KK179 pKa = 10.89 AKK181 pKa = 9.81 VKK183 pKa = 10.6 HH184 pKa = 5.76 FNNDD188 pKa = 2.8 EE189 pKa = 4.08 LKK191 pKa = 10.82 QFLTYY196 pKa = 10.54 LDD198 pKa = 4.21 SLDD201 pKa = 3.38 NTKK204 pKa = 10.86 YY205 pKa = 8.96 NTYY208 pKa = 10.95 YY209 pKa = 10.97 DD210 pKa = 3.1 ITLYY214 pKa = 10.96 KK215 pKa = 10.3 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.86 IDD236 pKa = 4.49 LDD238 pKa = 3.79 NSVVHH243 pKa = 5.55 ITKK246 pKa = 9.15 TLNCEE251 pKa = 3.99 MKK253 pKa = 10.84 LNSPKK258 pKa = 10.56 SKK260 pKa = 10.47 SSYY263 pKa = 10.18 RR264 pKa = 11.84 DD265 pKa = 2.8 IDD267 pKa = 3.19 IDD269 pKa = 3.79 RR270 pKa = 11.84 QTVTMLKK277 pKa = 10.06 KK278 pKa = 9.84 YY279 pKa = 8.29 QRR281 pKa = 11.84 KK282 pKa = 6.06 QTQEE286 pKa = 3.04 AWKK289 pKa = 10.12 LGRR292 pKa = 11.84 TEE294 pKa = 4.01 TVVFSNFIKK303 pKa = 10.5 AYY305 pKa = 9.64 PRR307 pKa = 11.84 INQLTKK313 pKa = 10.49 RR314 pKa = 11.84 LRR316 pKa = 11.84 AHH318 pKa = 6.77 FDD320 pKa = 2.99 KK321 pKa = 11.39 AGVNNIGFHH330 pKa = 5.79 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.0 KK348 pKa = 10.34 EE349 pKa = 3.8 LQHH352 pKa = 6.88 RR353 pKa = 11.84 LGHH356 pKa = 6.17 SKK358 pKa = 11.11 LSMTMDD364 pKa = 4.19 TYY366 pKa = 11.8 SHH368 pKa = 7.15 LSKK371 pKa = 11.01 EE372 pKa = 4.3 NAKK375 pKa = 10.32 KK376 pKa = 9.29 ATSFFEE382 pKa = 4.04 QALKK386 pKa = 10.82 SII388 pKa = 4.53
Molecular weight: 44.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.048
IPC2_protein 9.326
IPC_protein 9.194
Toseland 10.087
ProMoST 9.677
Dawson 10.248
Bjellqvist 9.867
Wikipedia 10.379
Rodwell 10.877
Grimsley 10.306
Solomon 10.262
Lehninger 10.233
Nozaki 10.058
DTASelect 9.867
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.145
Patrickios 10.496
IPC_peptide 10.262
IPC2_peptide 8.317
IPC2.peptide.svr19 8.304
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2383
50
388
148.9
17.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.211 ± 0.468
0.504 ± 0.124
6.043 ± 0.485
7.679 ± 0.817
4.448 ± 0.489
4.574 ± 0.579
1.804 ± 0.302
7.176 ± 0.512
9.316 ± 0.711
9.232 ± 0.541
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.308 ± 0.337
5.959 ± 0.425
2.728 ± 0.575
4.029 ± 0.298
5.329 ± 0.641
5.875 ± 0.459
6.63 ± 0.729
5.12 ± 0.4
0.713 ± 0.166
4.322 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here