Tortoise microvirus 91

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W725|A0A4P8W725_9VIRU Major capsid protein OS=Tortoise microvirus 91 OX=2583200 PE=3 SV=1
MM1 pKa = 6.99KK2 pKa = 9.45TIPFKK7 pKa = 10.9TPFNSIPDD15 pKa = 3.72EE16 pKa = 4.71GEE18 pKa = 4.05VNLDD22 pKa = 3.54PSEE25 pKa = 4.17TLPDD29 pKa = 3.4QSLSIMEE36 pKa = 4.09ILQRR40 pKa = 11.84HH41 pKa = 5.72AKK43 pKa = 9.77GLPLDD48 pKa = 3.54VKK50 pKa = 10.32MRR52 pKa = 11.84EE53 pKa = 4.29DD54 pKa = 3.58VGGGPDD60 pKa = 4.88DD61 pKa = 5.44FYY63 pKa = 10.97PDD65 pKa = 4.32PSTLDD70 pKa = 3.53LADD73 pKa = 4.31RR74 pKa = 11.84EE75 pKa = 4.48AFADD79 pKa = 3.7EE80 pKa = 4.51AKK82 pKa = 10.57RR83 pKa = 11.84EE84 pKa = 3.93LRR86 pKa = 11.84QIHH89 pKa = 6.97RR90 pKa = 11.84DD91 pKa = 3.21RR92 pKa = 11.84EE93 pKa = 3.84QRR95 pKa = 11.84KK96 pKa = 8.14QKK98 pKa = 10.51KK99 pKa = 9.92GSQKK103 pKa = 9.9PANPPKK109 pKa = 10.4KK110 pKa = 9.98DD111 pKa = 3.98DD112 pKa = 4.26PPKK115 pKa = 10.67KK116 pKa = 10.25DD117 pKa = 3.74DD118 pKa = 4.34TIQDD122 pKa = 3.66AEE124 pKa = 4.17IVEE127 pKa = 4.37

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6X6|A0A4P8W6X6_9VIRU Uncharacterized protein OS=Tortoise microvirus 91 OX=2583200 PE=4 SV=1
MM1 pKa = 7.7ACFTPMYY8 pKa = 10.15LEE10 pKa = 3.77QHH12 pKa = 5.54NVYY15 pKa = 9.56VPCGKK20 pKa = 10.5CPGCHH25 pKa = 6.01SARR28 pKa = 11.84ASAWSFRR35 pKa = 11.84MQQEE39 pKa = 4.31AKK41 pKa = 9.8ISTSAYY47 pKa = 9.72FVTLTYY53 pKa = 8.76DD54 pKa = 3.53TKK56 pKa = 10.84HH57 pKa = 6.79VPITPNGFMSLSKK70 pKa = 10.26RR71 pKa = 11.84DD72 pKa = 3.48CQLFFKK78 pKa = 10.52RR79 pKa = 11.84LRR81 pKa = 11.84KK82 pKa = 9.33NWPQTIRR89 pKa = 11.84YY90 pKa = 8.12YY91 pKa = 11.09LAGEE95 pKa = 4.2YY96 pKa = 10.46GDD98 pKa = 3.97SFNRR102 pKa = 11.84PHH104 pKa = 5.88YY105 pKa = 10.37HH106 pKa = 6.45VILFNSDD113 pKa = 3.05PQRR116 pKa = 11.84IVDD119 pKa = 3.19AWGNGDD125 pKa = 3.48VHH127 pKa = 7.89IADD130 pKa = 4.54VNPKK134 pKa = 8.08TCAYY138 pKa = 7.13TLKK141 pKa = 10.69YY142 pKa = 7.94ITKK145 pKa = 9.47GRR147 pKa = 11.84WKK149 pKa = 10.06PMHH152 pKa = 6.55KK153 pKa = 9.81RR154 pKa = 11.84DD155 pKa = 4.0DD156 pKa = 3.8RR157 pKa = 11.84APLFSLMSKK166 pKa = 10.74GLGKK170 pKa = 10.34NYY172 pKa = 8.85LTEE175 pKa = 5.05QMQDD179 pKa = 2.46WHH181 pKa = 6.71KK182 pKa = 11.18ANPAQRR188 pKa = 11.84VYY190 pKa = 9.8CTTSEE195 pKa = 4.35GVKK198 pKa = 8.84ITMPRR203 pKa = 11.84YY204 pKa = 9.17YY205 pKa = 10.04KK206 pKa = 10.67DD207 pKa = 3.21KK208 pKa = 10.36IYY210 pKa = 10.95SEE212 pKa = 4.55EE213 pKa = 3.83EE214 pKa = 3.46RR215 pKa = 11.84ALIAEE220 pKa = 4.77HH221 pKa = 6.68GLDD224 pKa = 3.47RR225 pKa = 11.84QTEE228 pKa = 4.18EE229 pKa = 4.06QRR231 pKa = 11.84KK232 pKa = 8.16QRR234 pKa = 11.84AANPNHH240 pKa = 6.61DD241 pKa = 4.5RR242 pKa = 11.84DD243 pKa = 3.74RR244 pKa = 11.84EE245 pKa = 4.21QYY247 pKa = 10.71VLNQFVKK254 pKa = 11.09AKK256 pKa = 8.02MTIKK260 pKa = 10.57KK261 pKa = 10.11

Molecular weight: 30.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1203

127

503

300.8

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.063 ± 0.978

0.831 ± 0.52

5.736 ± 1.259

5.902 ± 0.558

3.99 ± 0.632

5.819 ± 0.586

2.078 ± 0.594

5.486 ± 0.413

5.736 ± 1.252

7.232 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.741 ± 0.278

5.902 ± 1.377

6.401 ± 1.365

5.736 ± 1.526

6.318 ± 0.705

5.569 ± 0.264

5.985 ± 0.534

4.239 ± 0.334

1.164 ± 0.47

4.073 ± 0.975
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski