Staphylococcus phage vB_SsapH-Golestan101-M

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Baoshanvirus; unclassified Baoshanvirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 205 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S6MFK8|A0A6S6MFK8_9CAUD DNA polymerase I OS=Staphylococcus phage vB_SsapH-Golestan101-M OX=2741341 PE=4 SV=1
MM1 pKa = 7.06ITEE4 pKa = 4.06KK5 pKa = 10.31QNRR8 pKa = 11.84IIDD11 pKa = 3.7VLEE14 pKa = 4.32NEE16 pKa = 4.3GLEE19 pKa = 4.09VDD21 pKa = 5.0GSQEE25 pKa = 3.95YY26 pKa = 10.98YY27 pKa = 10.03IVEE30 pKa = 4.41DD31 pKa = 4.05SEE33 pKa = 4.61GHH35 pKa = 6.68SITLDD40 pKa = 3.65FVEE43 pKa = 5.66DD44 pKa = 4.16YY45 pKa = 10.79YY46 pKa = 11.94AEE48 pKa = 4.27ILDD51 pKa = 3.68NDD53 pKa = 3.58LRR55 pKa = 11.84KK56 pKa = 8.61TIVSLDD62 pKa = 3.66QEE64 pKa = 4.55HH65 pKa = 6.84LLVTEE70 pKa = 4.37VLDD73 pKa = 4.11ALDD76 pKa = 3.79YY77 pKa = 11.05SLEE80 pKa = 4.19EE81 pKa = 4.0VSLL84 pKa = 3.95

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S6MFG0|A0A6S6MFG0_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SsapH-Golestan101-M OX=2741341 PE=4 SV=1
MM1 pKa = 7.18TKK3 pKa = 10.34KK4 pKa = 10.44NKK6 pKa = 9.68PFVNYY11 pKa = 10.47APLGNMLGVNNKK23 pKa = 9.26PLFYY27 pKa = 10.37KK28 pKa = 10.43RR29 pKa = 11.84NKK31 pKa = 10.0VEE33 pKa = 4.27INDD36 pKa = 3.62SAKK39 pKa = 9.56TIKK42 pKa = 10.76YY43 pKa = 9.34KK44 pKa = 10.17IAFVKK49 pKa = 10.28VYY51 pKa = 10.79KK52 pKa = 10.58NVSTKK57 pKa = 10.71DD58 pKa = 3.34LSLQRR63 pKa = 11.84KK64 pKa = 8.01YY65 pKa = 11.37ALNLITDD72 pKa = 4.37FLNIKK77 pKa = 8.61EE78 pKa = 4.07QFLTIRR84 pKa = 11.84QHH86 pKa = 7.03KK87 pKa = 7.83KK88 pKa = 8.76TEE90 pKa = 5.21DD91 pKa = 3.16ILHH94 pKa = 6.63TDD96 pKa = 2.73RR97 pKa = 11.84VYY99 pKa = 11.16YY100 pKa = 9.53IRR102 pKa = 11.84KK103 pKa = 8.99GKK105 pKa = 10.07KK106 pKa = 9.78LLGKK110 pKa = 10.41CSIRR114 pKa = 11.84EE115 pKa = 3.85QRR117 pKa = 11.84TFTQTNLIIIFRR129 pKa = 11.84YY130 pKa = 8.36RR131 pKa = 11.84VRR133 pKa = 11.84VSGRR137 pKa = 11.84KK138 pKa = 8.97ARR140 pKa = 11.84SLTNEE145 pKa = 4.18SKK147 pKa = 10.67DD148 pKa = 3.56QKK150 pKa = 11.03KK151 pKa = 10.15GG152 pKa = 3.38

Molecular weight: 17.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

205

0

205

41108

30

1450

200.5

23.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.729 ± 0.191

0.642 ± 0.074

6.643 ± 0.167

8.874 ± 0.29

4.036 ± 0.135

5.729 ± 0.387

1.54 ± 0.098

7.427 ± 0.216

9.378 ± 0.279

7.969 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.142

6.96 ± 0.156

2.654 ± 0.138

3.819 ± 0.201

3.909 ± 0.125

6.096 ± 0.174

6.118 ± 0.204

5.95 ± 0.198

0.817 ± 0.064

5.337 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski