Thermococcus barophilus (strain DSM 11836 / MP)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Thermococcus; Thermococcus barophilus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0LJ45|F0LJ45_THEBM Threonine synthase OS=Thermococcus barophilus (strain DSM 11836 / MP) OX=391623 GN=TERMP_00414 PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.0VRR4 pKa = 11.84KK5 pKa = 9.01IAALAVGAAMVGATMGFASAQANLPGKK32 pKa = 10.24DD33 pKa = 3.57FFVKK37 pKa = 10.47DD38 pKa = 3.44GQPNVKK44 pKa = 9.58IVVGSQAAAMDD55 pKa = 3.88VASAADD61 pKa = 3.28IAVALGSLLYY71 pKa = 9.87TEE73 pKa = 5.42KK74 pKa = 10.36EE75 pKa = 3.84AEE77 pKa = 3.97AAGVSVVVKK86 pKa = 10.71KK87 pKa = 10.77DD88 pKa = 3.22LTPDD92 pKa = 3.1YY93 pKa = 9.82TYY95 pKa = 10.69YY96 pKa = 10.34IPVFSNYY103 pKa = 9.92YY104 pKa = 9.95EE105 pKa = 4.17DD106 pKa = 3.79TGTNPSATAWDD117 pKa = 3.8QLTDD121 pKa = 3.38NWWNGSAYY129 pKa = 10.33NGSYY133 pKa = 10.25TDD135 pKa = 3.41WTSWTPKK142 pKa = 10.24FVDD145 pKa = 3.59EE146 pKa = 4.74VEE148 pKa = 4.39NMDD151 pKa = 5.77AINGDD156 pKa = 4.04YY157 pKa = 10.36QVDD160 pKa = 3.34WDD162 pKa = 4.01FTISNIEE169 pKa = 4.14LSDD172 pKa = 3.86PEE174 pKa = 4.33QNTIVYY180 pKa = 8.87VPRR183 pKa = 11.84SADD186 pKa = 3.37LTIPAGDD193 pKa = 3.5FTVLLNYY200 pKa = 9.34TIANWTYY207 pKa = 10.9SATEE211 pKa = 4.18PDD213 pKa = 4.94SIWGTLNPSTVYY225 pKa = 11.07DD226 pKa = 4.18EE227 pKa = 5.06VHH229 pKa = 7.46DD230 pKa = 4.81DD231 pKa = 4.43DD232 pKa = 5.69NPGGYY237 pKa = 7.79TFSGYY242 pKa = 10.14IYY244 pKa = 10.06DD245 pKa = 4.04GVGAGDD251 pKa = 3.82TFTVLGNTYY260 pKa = 10.58YY261 pKa = 10.33ILDD264 pKa = 3.81VLADD268 pKa = 4.76GIKK271 pKa = 10.1YY272 pKa = 10.14GHH274 pKa = 7.05DD275 pKa = 3.21HH276 pKa = 5.57GQVWFHH282 pKa = 6.83VGDD285 pKa = 3.6VKK287 pKa = 11.17EE288 pKa = 3.93FDD290 pKa = 3.67GYY292 pKa = 10.7KK293 pKa = 10.0IKK295 pKa = 10.88AVDD298 pKa = 3.47ISVSPSNKK306 pKa = 9.8ALFEE310 pKa = 3.76ITAPDD315 pKa = 3.69GRR317 pKa = 11.84SDD319 pKa = 4.05LVIISTDD326 pKa = 3.26EE327 pKa = 4.21GDD329 pKa = 3.33VDD331 pKa = 4.87ISTKK335 pKa = 9.65SDD337 pKa = 2.83KK338 pKa = 10.74FNPGEE343 pKa = 4.21VVLKK347 pKa = 10.9LDD349 pKa = 3.76DD350 pKa = 3.92TFVGIDD356 pKa = 3.62GNLIAQLEE364 pKa = 4.37VRR366 pKa = 11.84TNVVEE371 pKa = 3.99VHH373 pKa = 6.15TGDD376 pKa = 3.73EE377 pKa = 4.49LVSGWVVNFTIEE389 pKa = 4.03NNKK392 pKa = 9.3VKK394 pKa = 10.2WLTLTNANDD403 pKa = 3.96LSGSTLDD410 pKa = 3.71ILGKK414 pKa = 10.01YY415 pKa = 8.66KK416 pKa = 9.95MYY418 pKa = 11.15YY419 pKa = 9.96EE420 pKa = 4.4VDD422 pKa = 3.21SHH424 pKa = 6.14TLEE427 pKa = 4.57TDD429 pKa = 2.96DD430 pKa = 3.56TTYY433 pKa = 11.16YY434 pKa = 10.24AAKK437 pKa = 9.97AYY439 pKa = 9.66IVVKK443 pKa = 9.69PSEE446 pKa = 4.62PIIQTEE452 pKa = 3.99EE453 pKa = 4.13LKK455 pKa = 11.37VGDD458 pKa = 4.32EE459 pKa = 4.26VSDD462 pKa = 3.72TGWVVDD468 pKa = 4.06QIKK471 pKa = 10.54GGTYY475 pKa = 8.91TEE477 pKa = 4.22VTVMHH482 pKa = 6.26PTEE485 pKa = 5.31PITYY489 pKa = 9.33LDD491 pKa = 3.81TEE493 pKa = 4.56IDD495 pKa = 3.75PEE497 pKa = 4.99NIDD500 pKa = 3.59SNLILVGGPVANAVTKK516 pKa = 10.22YY517 pKa = 10.65LVDD520 pKa = 3.41NGYY523 pKa = 8.26STVDD527 pKa = 3.22WYY529 pKa = 11.79NSAGDD534 pKa = 3.36IEE536 pKa = 4.85YY537 pKa = 10.29IEE539 pKa = 5.35DD540 pKa = 3.69YY541 pKa = 11.02NGFGILIVAGKK552 pKa = 10.0DD553 pKa = 3.02RR554 pKa = 11.84YY555 pKa = 8.98ATRR558 pKa = 11.84EE559 pKa = 3.69AAKK562 pKa = 10.6QLMEE566 pKa = 4.0YY567 pKa = 10.18LANLSS572 pKa = 3.58

Molecular weight: 62.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0LJS1|F0LJS1_THEBM Peptidase_M28 domain-containing protein OS=Thermococcus barophilus (strain DSM 11836 / MP) OX=391623 GN=TERMP_01738 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.23RR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84KK7 pKa = 7.59WKK9 pKa = 10.02KK10 pKa = 8.58KK11 pKa = 10.27GRR13 pKa = 11.84MRR15 pKa = 11.84WKK17 pKa = 9.51WIKK20 pKa = 10.46KK21 pKa = 8.96RR22 pKa = 11.84IRR24 pKa = 11.84RR25 pKa = 11.84LKK27 pKa = 9.33RR28 pKa = 11.84QRR30 pKa = 11.84KK31 pKa = 8.4KK32 pKa = 10.86EE33 pKa = 3.77RR34 pKa = 11.84GLII37 pKa = 3.84

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2265

0

2265

631540

37

2030

278.8

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.051

0.585 ± 0.018

4.523 ± 0.035

8.691 ± 0.075

4.617 ± 0.042

6.967 ± 0.048

1.613 ± 0.02

8.589 ± 0.045

8.368 ± 0.072

10.301 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.025

3.512 ± 0.043

4.064 ± 0.035

1.969 ± 0.027

5.049 ± 0.044

4.823 ± 0.046

4.356 ± 0.046

7.497 ± 0.044

1.187 ± 0.019

4.069 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski