Oscillatoria nigro-viridis PCC 7112
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6276 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9VTE3|K9VTE3_9CYAN Uncharacterized protein OS=Oscillatoria nigro-viridis PCC 7112 OX=179408 GN=Osc7112_6734 PE=4 SV=1
MM1 pKa = 7.86 SLQQCEE7 pKa = 4.6 ADD9 pKa = 3.68 DD10 pKa = 3.96 TTNTLISYY18 pKa = 7.67 TNTSLLGRR26 pKa = 11.84 VDD28 pKa = 4.78 FKK30 pKa = 11.5 SDD32 pKa = 3.34 SLEE35 pKa = 4.74 LIPEE39 pKa = 4.22 TEE41 pKa = 3.76 ILEE44 pKa = 4.44 VGDD47 pKa = 4.27 EE48 pKa = 4.65 SIDD51 pKa = 3.53 SHH53 pKa = 7.12 SLEE56 pKa = 5.67 DD57 pKa = 3.88 EE58 pKa = 4.3 LTPGTLAILASIEE71 pKa = 4.09 TFDD74 pKa = 5.38 EE75 pKa = 4.41 YY76 pKa = 10.81 FHH78 pKa = 7.57 CCGRR82 pKa = 11.84 WW83 pKa = 2.95
Molecular weight: 9.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.808
IPC_protein 3.732
Toseland 3.554
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.63
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.643
Sillero 3.859
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|K9VC83|K9VC83_9CYAN Amino acid/amide ABC transporter substrate-binding protein HAAT family OS=Oscillatoria nigro-viridis PCC 7112 OX=179408 GN=Osc7112_0495 PE=4 SV=1
MM1 pKa = 6.86 TQQTLHH7 pKa = 5.03 GTSRR11 pKa = 11.84 KK12 pKa = 9.46 RR13 pKa = 11.84 KK14 pKa = 7.05 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TVTGRR29 pKa = 11.84 LVIKK33 pKa = 10.38 ARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.48 GRR40 pKa = 11.84 HH41 pKa = 4.61 RR42 pKa = 11.84 LAVV45 pKa = 3.37
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6276
0
6276
2109055
30
7380
336.1
37.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.693 ± 0.033
1.048 ± 0.013
5.08 ± 0.03
6.488 ± 0.037
3.879 ± 0.018
6.87 ± 0.043
1.637 ± 0.018
6.394 ± 0.027
4.914 ± 0.031
10.624 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.825 ± 0.018
4.342 ± 0.032
4.895 ± 0.034
4.959 ± 0.029
5.289 ± 0.033
6.612 ± 0.032
5.581 ± 0.053
6.589 ± 0.025
1.372 ± 0.015
2.911 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here