Sea otter polyomavirus 1
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3KGJ2|A0A0S3KGJ2_9POLY Capsid protein VP1 OS=Sea otter polyomavirus 1 OX=1552409 PE=3 SV=1
MM1 pKa = 7.57 DD2 pKa = 3.91 TVLSRR7 pKa = 11.84 EE8 pKa = 4.3 DD9 pKa = 3.25 ARR11 pKa = 11.84 EE12 pKa = 3.79 LMGLLRR18 pKa = 11.84 LDD20 pKa = 3.24 MAVYY24 pKa = 10.77 GNLPLMRR31 pKa = 11.84 KK32 pKa = 9.6 AYY34 pKa = 10.35 LKK36 pKa = 10.23 RR37 pKa = 11.84 CKK39 pKa = 9.66 EE40 pKa = 3.83 LHH42 pKa = 6.38 PDD44 pKa = 3.2 KK45 pKa = 11.49 GGDD48 pKa = 3.17 EE49 pKa = 4.3 TMMKK53 pKa = 10.2 KK54 pKa = 10.58 LNEE57 pKa = 4.17 LYY59 pKa = 10.96 KK60 pKa = 11.07 LMEE63 pKa = 4.78 DD64 pKa = 4.42 NIRR67 pKa = 11.84 EE68 pKa = 3.99 VHH70 pKa = 6.5 SNVEE74 pKa = 3.76 AAGWKK79 pKa = 9.99 AEE81 pKa = 3.94 EE82 pKa = 4.38 VPTYY86 pKa = 9.61 GTTEE90 pKa = 3.86 WEE92 pKa = 4.4 HH93 pKa = 4.47 WWEE96 pKa = 3.99 AFNANWNDD104 pKa = 3.33 LRR106 pKa = 11.84 CDD108 pKa = 3.58 EE109 pKa = 4.46 EE110 pKa = 5.81 LEE112 pKa = 4.69 EE113 pKa = 4.8 EE114 pKa = 4.84 DD115 pKa = 5.88 LDD117 pKa = 4.98 DD118 pKa = 4.68 SQHH121 pKa = 5.37 STPPKK126 pKa = 8.79 KK127 pKa = 9.9 KK128 pKa = 10.08 RR129 pKa = 11.84 RR130 pKa = 11.84 GDD132 pKa = 3.49 EE133 pKa = 3.96 PKK135 pKa = 10.66 DD136 pKa = 3.66 FPEE139 pKa = 4.27 SLRR142 pKa = 11.84 TMLSQAIFSNRR153 pKa = 11.84 TLSCFAIYY161 pKa = 7.53 TTSDD165 pKa = 2.95 KK166 pKa = 11.34 ARR168 pKa = 11.84 LLFKK172 pKa = 10.96 KK173 pKa = 10.73 LMDD176 pKa = 4.02 KK177 pKa = 11.06 YY178 pKa = 10.36 SANFISLHH186 pKa = 5.37 SCGIHH191 pKa = 6.97 SILFLLTPQKK201 pKa = 10.8 HH202 pKa = 4.9 RR203 pKa = 11.84 VSAINNFCLKK213 pKa = 10.2 FCTISFLICKK223 pKa = 9.0 GVNKK227 pKa = 9.74 EE228 pKa = 3.81 YY229 pKa = 10.87 CLYY232 pKa = 10.72 KK233 pKa = 10.61 ALCTDD238 pKa = 4.1 PFKK241 pKa = 11.12 LLQEE245 pKa = 4.31 SVEE248 pKa = 4.44 GGLSEE253 pKa = 4.42 NYY255 pKa = 9.87 FMPEE259 pKa = 3.32 EE260 pKa = 4.38 GDD262 pKa = 3.65 EE263 pKa = 4.28 VKK265 pKa = 10.54 QVSWKK270 pKa = 9.99 MITEE274 pKa = 4.03 YY275 pKa = 10.8 AVEE278 pKa = 4.17 IDD280 pKa = 5.24 CDD282 pKa = 3.94 DD283 pKa = 4.92 LYY285 pKa = 11.99 LLMGLYY291 pKa = 10.33 LEE293 pKa = 5.04 YY294 pKa = 9.9 ATEE297 pKa = 4.01 PDD299 pKa = 3.31 YY300 pKa = 11.76 CKK302 pKa = 10.18 ICSSKK307 pKa = 10.62 SIPVHH312 pKa = 5.02 YY313 pKa = 9.87 RR314 pKa = 11.84 NHH316 pKa = 6.39 GKK318 pKa = 8.39 HH319 pKa = 6.35 HH320 pKa = 6.36 GNALLFLEE328 pKa = 4.99 SKK330 pKa = 9.11 NQKK333 pKa = 9.97 AICQQAVDD341 pKa = 3.66 TVIAKK346 pKa = 10.02 KK347 pKa = 10.28 RR348 pKa = 11.84 VEE350 pKa = 4.11 TKK352 pKa = 9.65 YY353 pKa = 10.85 LSRR356 pKa = 11.84 ADD358 pKa = 3.55 MLTKK362 pKa = 10.35 RR363 pKa = 11.84 FHH365 pKa = 6.89 KK366 pKa = 10.3 LLDD369 pKa = 3.34 MMEE372 pKa = 4.63 DD373 pKa = 3.3 MFGASGSADD382 pKa = 3.41 LKK384 pKa = 11.33 LYY386 pKa = 9.83 MCGCAWLEE394 pKa = 3.79 CLQYY398 pKa = 11.17 CMEE401 pKa = 4.22 SVVYY405 pKa = 10.04 EE406 pKa = 4.55 FLRR409 pKa = 11.84 TMVTNIPKK417 pKa = 9.67 KK418 pKa = 10.39 RR419 pKa = 11.84 YY420 pKa = 8.08 WLFKK424 pKa = 10.95 GPIDD428 pKa = 3.83 TGKK431 pKa = 7.19 TTLAAGLLNLCGGRR445 pKa = 11.84 ALNINLPQDD454 pKa = 3.49 RR455 pKa = 11.84 LPFEE459 pKa = 4.86 LGVAIDD465 pKa = 3.29 QFMVVFEE472 pKa = 4.44 DD473 pKa = 3.83 VKK475 pKa = 11.04 GHH477 pKa = 6.51 GGRR480 pKa = 11.84 DD481 pKa = 3.59 LPPGQGVHH489 pKa = 6.25 NLDD492 pKa = 3.49 NLRR495 pKa = 11.84 DD496 pKa = 3.88 YY497 pKa = 11.6 LDD499 pKa = 4.13 GSVKK503 pKa = 10.37 VNLEE507 pKa = 3.85 KK508 pKa = 10.85 KK509 pKa = 9.59 HH510 pKa = 5.0 VNKK513 pKa = 9.3 RR514 pKa = 11.84 TQIFPPGLVTMNEE527 pKa = 3.5 YY528 pKa = 10.7 DD529 pKa = 3.42 IPRR532 pKa = 11.84 TLLARR537 pKa = 11.84 FARR540 pKa = 11.84 IVEE543 pKa = 4.37 FRR545 pKa = 11.84 PKK547 pKa = 9.9 PHH549 pKa = 7.36 LKK551 pKa = 10.19 RR552 pKa = 11.84 SLNNTPEE559 pKa = 4.33 LLEE562 pKa = 3.85 QRR564 pKa = 11.84 IVQSGVTLLLLLIWHH579 pKa = 6.65 RR580 pKa = 11.84 PVYY583 pKa = 10.83 DD584 pKa = 3.69 FDD586 pKa = 4.85 EE587 pKa = 5.68 DD588 pKa = 3.22 IQARR592 pKa = 11.84 VVEE595 pKa = 4.53 WKK597 pKa = 10.15 EE598 pKa = 3.82 RR599 pKa = 11.84 LDD601 pKa = 4.13 SEE603 pKa = 5.15 IPCTMYY609 pKa = 11.07 SRR611 pKa = 11.84 MKK613 pKa = 10.59 RR614 pKa = 11.84 NVACGVHH621 pKa = 6.06 ILSDD625 pKa = 3.46 TADD628 pKa = 3.38 EE629 pKa = 4.62 GRR631 pKa = 11.84 AFRR634 pKa = 11.84 PQPPPPPQPADD645 pKa = 3.63 DD646 pKa = 4.55 SEE648 pKa = 6.05 ADD650 pKa = 3.42 SGMDD654 pKa = 3.33 SARR657 pKa = 11.84 SQSSSEE663 pKa = 4.09 DD664 pKa = 3.5 TDD666 pKa = 4.97 DD667 pKa = 4.3 GFPPVPPPSPVPFF680 pKa = 4.65
Molecular weight: 77.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.571
IPC2_protein 5.575
IPC_protein 5.601
Toseland 5.881
ProMoST 5.906
Dawson 5.804
Bjellqvist 5.842
Wikipedia 5.792
Rodwell 5.779
Grimsley 5.97
Solomon 5.804
Lehninger 5.792
Nozaki 6.046
DTASelect 6.237
Thurlkill 6.198
EMBOSS 6.16
Sillero 6.148
Patrickios 4.075
IPC_peptide 5.83
IPC2_peptide 6.135
IPC2.peptide.svr19 6.164
Protein with the highest isoelectric point:
>tr|A0A097A5I9|A0A097A5I9_9POLY Large T antigen OS=Sea otter polyomavirus 1 OX=1552409 PE=4 SV=1
MM1 pKa = 7.69 ALQVWYY7 pKa = 9.71 PDD9 pKa = 3.57 FQEE12 pKa = 4.03 MLFPGVNSLVRR23 pKa = 11.84 ALHH26 pKa = 5.96 YY27 pKa = 10.16 IDD29 pKa = 3.85 PRR31 pKa = 11.84 EE32 pKa = 4.07 WAASLYY38 pKa = 10.7 AQLRR42 pKa = 11.84 DD43 pKa = 3.55 LFGYY47 pKa = 10.3 GPARR51 pKa = 11.84 LGYY54 pKa = 8.33 STTEE58 pKa = 3.7 VGQATGISFRR68 pKa = 11.84 DD69 pKa = 3.48 SVARR73 pKa = 11.84 LLEE76 pKa = 4.19 EE77 pKa = 4.14 VTWAVSSAPLRR88 pKa = 11.84 TYY90 pKa = 10.9 QFLADD95 pKa = 3.95 YY96 pKa = 9.94 YY97 pKa = 11.24 SSLSHH102 pKa = 6.45 IRR104 pKa = 11.84 PSMVRR109 pKa = 11.84 QIAQSHH115 pKa = 6.3 GPLPSKK121 pKa = 10.73 FIDD124 pKa = 3.94 YY125 pKa = 10.8 KK126 pKa = 11.39 NLDD129 pKa = 3.67 NGPFSGEE136 pKa = 4.28 LVDD139 pKa = 5.18 KK140 pKa = 10.93 APPPGGANQRR150 pKa = 11.84 VTPDD154 pKa = 2.78 WLLPLILDD162 pKa = 4.76 LYY164 pKa = 11.14 GPLNPSWKK172 pKa = 10.11 EE173 pKa = 3.86 EE174 pKa = 4.09 EE175 pKa = 4.87 DD176 pKa = 4.49 DD177 pKa = 4.58 GPQKK181 pKa = 10.63 KK182 pKa = 8.96 IQRR185 pKa = 11.84 TNTRR189 pKa = 11.84 AKK191 pKa = 10.61 APNKK195 pKa = 9.29 RR196 pKa = 11.84 RR197 pKa = 11.84 NRR199 pKa = 11.84 GSGGQKK205 pKa = 9.46 RR206 pKa = 11.84 ARR208 pKa = 11.84 KK209 pKa = 9.41 HH210 pKa = 5.19 SRR212 pKa = 11.84 NN213 pKa = 3.5
Molecular weight: 24.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.866
IPC2_protein 9.165
IPC_protein 9.253
Toseland 9.589
ProMoST 9.458
Dawson 9.897
Bjellqvist 9.619
Wikipedia 10.131
Rodwell 10.072
Grimsley 10.014
Solomon 9.94
Lehninger 9.882
Nozaki 9.545
DTASelect 9.633
Thurlkill 9.706
EMBOSS 10.028
Sillero 9.809
Patrickios 5.003
IPC_peptide 9.926
IPC2_peptide 8.229
IPC2.peptide.svr19 8.059
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1823
69
680
303.8
34.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.308 ± 1.362
2.139 ± 0.742
5.979 ± 0.543
6.363 ± 0.564
3.511 ± 0.235
7.076 ± 0.966
2.194 ± 0.222
4.059 ± 0.332
5.979 ± 0.795
10.697 ± 0.568
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.852 ± 0.581
4.114 ± 0.197
6.418 ± 0.701
3.62 ± 0.778
6.089 ± 0.436
6.473 ± 0.614
4.882 ± 0.367
5.595 ± 0.412
1.81 ± 0.308
3.84 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here