Xiburema virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Arurhavirus; Xiburema arurhavirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059U1X5|A0A059U1X5_9RHAB RNA-directed RNA polymerase L OS=Xiburema virus OX=1272959 PE=3 SV=1
MM1 pKa = 8.0DD2 pKa = 5.37PANLVRR8 pKa = 11.84FGSKK12 pKa = 10.09GRR14 pKa = 11.84FPNMKK19 pKa = 9.99LAIEE23 pKa = 4.47DD24 pKa = 3.57MLMTEE29 pKa = 4.51SALEE33 pKa = 4.19AEE35 pKa = 4.89SNPINNQPAPLLTSFLAEE53 pKa = 3.55VDD55 pKa = 3.96GQNLDD60 pKa = 3.44AAAEE64 pKa = 4.11EE65 pKa = 4.35DD66 pKa = 3.45WGEE69 pKa = 4.08KK70 pKa = 9.01VAQEE74 pKa = 4.25VFQGDD79 pKa = 3.6WDD81 pKa = 4.06MEE83 pKa = 4.66DD84 pKa = 3.6GPKK87 pKa = 9.14FTFLLNYY94 pKa = 9.53LPPDD98 pKa = 3.33LRR100 pKa = 11.84DD101 pKa = 3.44QVEE104 pKa = 4.32SSVLNLLDD112 pKa = 4.26WINTSSRR119 pKa = 11.84VGLYY123 pKa = 8.04MRR125 pKa = 11.84RR126 pKa = 11.84TEE128 pKa = 3.91SSVEE132 pKa = 3.7IKK134 pKa = 10.54YY135 pKa = 8.68EE136 pKa = 4.1KK137 pKa = 9.91PDD139 pKa = 3.65EE140 pKa = 4.65KK141 pKa = 10.83DD142 pKa = 3.14PPLAPVQIPEE152 pKa = 4.31PVPSSSAQKK161 pKa = 10.84LNLNKK166 pKa = 7.78EE167 pKa = 4.62TPVKK171 pKa = 10.56KK172 pKa = 9.88PIKK175 pKa = 9.84LAPTFNEE182 pKa = 4.02EE183 pKa = 3.85RR184 pKa = 11.84CISLGEE190 pKa = 4.27GKK192 pKa = 10.28SGEE195 pKa = 4.16ALYY198 pKa = 11.24LNLKK202 pKa = 9.99HH203 pKa = 6.22GVKK206 pKa = 10.22FPKK209 pKa = 9.55RR210 pKa = 11.84TGSGHH215 pKa = 6.75LKK217 pKa = 10.59VSLEE221 pKa = 4.1TPGIKK226 pKa = 10.38DD227 pKa = 3.63ADD229 pKa = 3.36IEE231 pKa = 4.39KK232 pKa = 10.44YY233 pKa = 10.41KK234 pKa = 10.84NYY236 pKa = 9.3PLKK239 pKa = 10.86EE240 pKa = 4.15GLHH243 pKa = 6.19KK244 pKa = 10.49LLKK247 pKa = 10.36RR248 pKa = 11.84AGIWKK253 pKa = 9.76ALVGVADD260 pKa = 3.82VEE262 pKa = 4.48KK263 pKa = 10.37PIWPDD268 pKa = 2.85LRR270 pKa = 11.84YY271 pKa = 10.2

Molecular weight: 30.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059TZZ5|A0A059TZZ5_9RHAB Nucleocapsid protein OS=Xiburema virus OX=1272959 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 5.83RR3 pKa = 11.84GIGFNPSQAIDD14 pKa = 4.42GIKK17 pKa = 10.46GALNNLGNSISSFFNDD33 pKa = 3.25IGIKK37 pKa = 9.97LNYY40 pKa = 7.81WGKK43 pKa = 10.11IFLIIIGVILGLIIIPRR60 pKa = 11.84LISNVCTIISGLSKK74 pKa = 10.4CLRR77 pKa = 11.84WLWRR81 pKa = 11.84MVSACRR87 pKa = 11.84LRR89 pKa = 11.84CPRR92 pKa = 11.84LCCKK96 pKa = 10.07FRR98 pKa = 11.84RR99 pKa = 11.84GDD101 pKa = 3.27NN102 pKa = 3.38

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3865

65

2100

552.1

63.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.088 ± 0.515

1.785 ± 0.309

5.201 ± 0.398

6.442 ± 0.639

3.752 ± 0.359

6.261 ± 0.236

2.251 ± 0.396

7.995 ± 0.677

6.779 ± 0.307

9.832 ± 0.698

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.389 ± 0.347

5.408 ± 0.351

5.123 ± 0.752

3.596 ± 0.295

5.045 ± 0.308

7.4 ± 0.601

5.252 ± 0.397

5.019 ± 0.63

1.785 ± 0.183

3.596 ± 0.532
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski